FastQCFastQC Report
Fri 10 Feb 2017
ERR1630125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708165
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.4501775716111358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27220.3843736982200476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20140.28439699787478906No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18030.25460168181144227No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT13300.18780933821920032No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13010.18371424738584935No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC11940.16860477431107157No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.15759039206964479No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC10970.1549074015236562No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG10730.15151836083398645No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10570.14925900037420656No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG10220.14431664936843813No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC10140.14318696913854823No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT9810.13852703819025228No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA9620.1358440476442637No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG9470.1337258972132201No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC9420.13301984706953887No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG9400.1327374270120664No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC8610.12158183474190337No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8430.11904005422465104No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA8350.11791037399476112No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8060.11381528316141012No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8020.11325044304646517No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG7860.1109910825866853No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC7770.10972019232805914No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG7740.10929656224185041No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC7640.107884461954488No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT7400.10449542126481823No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG7370.1040717911786095No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC7240.10223606080503836No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC7240.10223606080503836No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA7230.10209485077630213No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC7210.10181243071882966No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGA7180.10138880063262092No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG7180.10138880063262092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT801.8189894E-1230.06251
TGCGCTA250.005494937829.59999810
ATATAGA250.005494937829.5999981
GTACGAT250.005494937829.59999820
TATATCT952.8012437E-1023.3684225
ATTAGGT400.00193045923.12519
CTCTGTA400.00193045923.1252
ATCGATA400.00193045923.12523
CTATATC1005.329639E-1022.1999994
CATTGCG450.003824378820.55555729
TATCTAT1101.7516868E-920.1818187
ACTATAT1202.382876E-1020.0416683
CGGGAGT2600.019.9230774
GACTCCT656.897347E-519.9230777
GGGTTAG851.2439268E-619.5882367
GGGAGTG2700.019.1851865
GTACTGG1355.638867E-1119.1851861
ATATAGT1650.019.0606041
ATCTATT1205.164111E-918.58
AATTATA1103.8453436E-818.528
Produced by FastQC (version 0.11.5)