##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630125.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708165
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.688044452917048	31.0	31.0	34.0	30.0	34.0
2	31.87884461954488	33.0	31.0	34.0	30.0	34.0
3	31.999956224891093	33.0	31.0	34.0	30.0	34.0
4	35.67614044749458	37.0	35.0	37.0	33.0	37.0
5	35.60924784478193	37.0	35.0	37.0	33.0	37.0
6	35.6651994944681	37.0	35.0	37.0	33.0	37.0
7	35.63571060416711	37.0	35.0	37.0	33.0	37.0
8	35.62224199162625	37.0	35.0	37.0	33.0	37.0
9	37.28234521615725	39.0	37.0	39.0	34.0	39.0
10	37.1872953337146	39.0	37.0	39.0	34.0	39.0
11	37.25854850211462	39.0	37.0	39.0	34.0	39.0
12	37.124079840150245	39.0	37.0	39.0	33.0	39.0
13	37.165625242704735	39.0	37.0	39.0	33.0	39.0
14	38.29920428148807	40.0	38.0	41.0	34.0	41.0
15	38.27388673543595	40.0	38.0	41.0	33.0	41.0
16	38.23983958540735	40.0	38.0	41.0	33.0	41.0
17	38.2178334145291	40.0	38.0	41.0	33.0	41.0
18	38.1864466614419	40.0	38.0	41.0	33.0	41.0
19	38.17228329555965	40.0	38.0	41.0	33.0	41.0
20	38.11768726214936	40.0	38.0	41.0	33.0	41.0
21	38.039096820656205	40.0	37.0	41.0	33.0	41.0
22	37.98744925264592	40.0	37.0	41.0	33.0	41.0
23	37.913123354020605	40.0	37.0	41.0	33.0	41.0
24	37.87569846010464	40.0	37.0	41.0	33.0	41.0
25	37.81168089357706	40.0	37.0	41.0	32.0	41.0
26	37.6070506167348	40.0	37.0	41.0	32.0	41.0
27	37.4816575233173	39.0	37.0	41.0	32.0	41.0
28	37.30749331017489	39.0	36.0	41.0	31.0	41.0
29	37.21210593576356	39.0	36.0	41.0	31.0	41.0
30	37.092653548254994	39.0	36.0	41.0	31.0	41.0
31	36.97670175735881	39.0	36.0	41.0	31.0	41.0
32	36.8474663390594	39.0	35.0	40.0	30.0	41.0
33	36.73902692169198	39.0	35.0	40.0	30.0	41.0
34	36.63752232883579	39.0	35.0	40.0	30.0	41.0
35	36.4698368318118	39.0	35.0	40.0	30.0	41.0
36	36.321163853056845	39.0	35.0	40.0	30.0	41.0
37	36.23596054591797	39.0	35.0	40.0	29.0	41.0
38	36.061738436663774	38.0	35.0	40.0	29.0	41.0
39	35.956226303192054	38.0	35.0	40.0	28.0	41.0
40	35.76472855902226	38.0	35.0	40.0	28.0	41.0
41	35.60306990602473	38.0	34.0	40.0	27.0	41.0
42	35.45436162476259	38.0	34.0	40.0	26.0	41.0
43	34.12859150056837	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	3.0
11	1.0
12	1.0
13	1.0
14	4.0
15	6.0
16	12.0
17	28.0
18	73.0
19	139.0
20	306.0
21	495.0
22	830.0
23	1289.0
24	2116.0
25	3120.0
26	4381.0
27	6194.0
28	8569.0
29	11027.0
30	14464.0
31	18391.0
32	23087.0
33	29466.0
34	38156.0
35	50187.0
36	68970.0
37	102688.0
38	167385.0
39	156774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.07790557285379	19.972181624338962	12.607796205686528	25.34211659712073
2	20.44735337103641	20.85756850451519	32.624882619163614	26.070195505284786
3	20.351330551495767	22.75232467009807	29.506117924494994	27.390226853911166
4	14.243573178567143	16.058122047827837	34.471062534861225	35.22724223874379
5	15.221170207508136	36.10585103754069	33.12674306129221	15.546235693658964
6	34.483347807361284	35.34642350299718	15.267487096933625	14.902741592707914
7	29.788679191996216	30.374135971136667	20.57543086710018	19.261753969766932
8	28.17733155408697	31.4570756815149	19.51423750114733	20.851355263250795
9	27.166550168392963	13.513234909943304	20.115933433592453	39.20428148807129
10	19.853282780143044	24.49316190435845	29.640126241765692	26.01342907373282
11	38.37693192970565	20.97279588796396	19.785925596435856	20.86434658589453
12	23.433239428664223	25.90003742065762	25.70488516094413	24.961837989734033
13	29.88272507113455	17.655066262805985	25.013803280308966	27.448405385750497
14	23.211539683548327	20.86702957644052	23.590264980618926	32.331165759392235
15	27.369892609773146	26.69617956267254	21.166959677476292	24.76696815007802
16	26.540707321033942	24.665861769502868	23.200666511335637	25.592764398127553
17	24.781653993066588	25.687375117380835	23.841901251826904	25.68906963772567
18	24.81427350970466	22.938863118058645	26.373373436981495	25.8734899352552
19	25.131148814188787	24.3694619191855	25.128889453729002	25.370499812896714
20	25.50154271956394	22.779578205644167	25.617193733098926	26.101685341692964
21	27.889827935579987	23.793325001941636	24.38555986246143	23.931287200016946
22	26.344284171061826	24.077157159701482	24.32780496070831	25.25075370852838
23	24.6624727288132	23.422366256451532	25.83465717735274	26.08050383738253
24	25.153177578671638	25.357226070195505	24.292361243495513	25.197235107637344
25	25.189186135999382	24.16809641820762	24.844068825768005	25.798648620024995
26	25.545035408414705	25.954544491749804	24.591585294387606	23.90883480544788
27	26.280598448101784	23.968425437574574	24.050468464270335	25.700507650053307
28	24.169790938552456	24.519144549645915	26.437624000056488	24.873440511745144
29	24.600763946255462	25.10135349812544	25.691328998185455	24.60655355743365
30	23.70619841421138	25.488268976862738	26.854758424943338	23.950774183982546
31	25.551954699822783	25.228724944045528	24.083370400965876	25.135949955165813
32	23.468400725819546	24.15877655631103	24.454611566513453	27.91821115135597
33	23.261104403634747	23.815918606539434	26.36701898568836	26.55595800413745
34	25.157837509619934	24.069531818149727	25.86035740258273	24.91227326964761
35	25.895801119795525	24.459130287433016	25.929409106634754	23.715659486136705
36	24.22486284975959	25.400436338988797	25.163909540855588	25.210791270396022
37	24.639596704157928	24.424110200306426	25.675937105053197	25.26035599048244
38	23.99003057197122	23.145877020185974	27.273022530060086	25.59106987778272
39	24.237430542317114	22.942675788834524	26.35473371318831	26.46515995566005
40	23.657339744268636	24.517026399214874	27.40914899776182	24.416484858754668
41	22.830696236046684	23.553550373147498	28.69048879851447	24.925264592291345
42	24.000338904068965	24.35830632691534	27.839839585407354	23.80151518360834
43	22.85385468075943	22.739756977540544	28.12466021336835	26.28172812833167
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	37.0
2	60.0
3	212.5
4	365.0
5	365.0
6	500.5
7	636.0
8	627.5
9	619.0
10	815.5
11	1012.0
12	1012.0
13	1982.5
14	2953.0
15	4744.0
16	6535.0
17	5488.5
18	4442.0
19	4442.0
20	4958.5
21	5475.0
22	3855.5
23	2236.0
24	2558.0
25	2880.0
26	2880.0
27	3505.0
28	4130.0
29	5034.0
30	5938.0
31	7867.5
32	9797.0
33	9797.0
34	13563.5
35	17330.0
36	22490.0
37	27650.0
38	32340.5
39	37031.0
40	37031.0
41	39536.0
42	42041.0
43	46351.0
44	50661.0
45	51722.5
46	52784.0
47	52784.0
48	55602.0
49	58420.0
50	56430.5
51	54441.0
52	54381.5
53	54322.0
54	54322.0
55	53773.0
56	53224.0
57	50877.0
58	48530.0
59	45960.5
60	43391.0
61	43391.0
62	38765.0
63	34139.0
64	30137.0
65	26135.0
66	22577.5
67	19020.0
68	19020.0
69	16622.0
70	14224.0
71	12696.5
72	11169.0
73	8763.5
74	6358.0
75	6358.0
76	4925.5
77	3493.0
78	2828.0
79	2163.0
80	1862.5
81	1562.0
82	1562.0
83	1178.5
84	795.0
85	787.5
86	780.0
87	740.0
88	700.0
89	700.0
90	656.0
91	612.0
92	338.5
93	65.0
94	45.0
95	25.0
96	25.0
97	16.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	708165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.520151965557005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35929405752805	21.49258518537797
2	11.336806623679635	6.466548954229529
3	4.083469893323429	3.493835457130831
4	2.0681387798176707	2.3593452914504645
5	1.2433087927755972	1.7729677855036632
6	0.8161941600504569	1.396678888682351
7	0.614208736244345	1.2262128547383009
8	0.47327382008892127	1.0798273016204576
9	0.3609845419190229	0.9265800593472765
>10	2.7087943795260974	15.9769607038619
>50	0.4432222767630901	8.990215073197406
>100	0.4585912818855888	26.63949672313316
>500	0.027267589733737708	5.360590007470247
>1k	0.006445066664338003	2.818155714256423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3188	0.4501775716111358	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2722	0.3843736982200476	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2014	0.28439699787478906	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1803	0.25460168181144227	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1330	0.18780933821920032	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1301	0.18371424738584935	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1194	0.16860477431107157	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1116	0.15759039206964479	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1097	0.1549074015236562	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1073	0.15151836083398645	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1057	0.14925900037420656	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1022	0.14431664936843813	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1014	0.14318696913854823	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	981	0.13852703819025228	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	962	0.1358440476442637	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	947	0.1337258972132201	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	942	0.13301984706953887	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	940	0.1327374270120664	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	861	0.12158183474190337	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	843	0.11904005422465104	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	835	0.11791037399476112	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	806	0.11381528316141012	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	802	0.11325044304646517	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	786	0.1109910825866853	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	777	0.10972019232805914	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG	774	0.10929656224185041	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	764	0.107884461954488	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT	740	0.10449542126481823	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	737	0.1040717911786095	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	724	0.10223606080503836	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	724	0.10223606080503836	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	723	0.10209485077630213	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	721	0.10181243071882966	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGA	718	0.10138880063262092	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	718	0.10138880063262092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4121002873624086E-4	0.0
11	0.0	0.0	0.0	1.4121002873624086E-4	0.0
12	0.0	0.0	0.0	1.4121002873624086E-4	0.0
13	0.0	0.0	0.0	2.824200574724817E-4	0.0
14	0.0	0.0	0.0	2.824200574724817E-4	0.0
15	0.0	0.0	0.0	5.648401149449634E-4	0.0
16	0.0	0.0	0.0	0.001976940402307372	0.0
17	0.0	0.0	0.0	0.0035302507184060213	0.0
18	0.0	0.0	0.0	0.003671460747142262	0.0
19	0.0	0.0	0.0	0.004801140977032188	0.0
20	0.0	0.0	0.0	0.005789611178185875	0.0
21	0.0	0.0	0.0	0.008048971637965729	0.0
22	0.0	0.0	0.0	0.012567692557525436	0.0
23	0.0	0.0	0.0	0.018216093706975068	0.0
24	0.0	0.0	0.0	0.027112325517358243	0.0
25	0.0	0.0	0.0	0.02993652609208306	0.0
26	0.0	0.0	0.0	0.034455247011642764	0.0
27	0.0	0.0	0.0	0.04829382982779437	0.0
28	0.0	0.0	0.0	0.09051562841993038	0.0
29	0.0	0.0	0.0	0.15208320094893138	0.0
30	0.0	0.0	0.0	0.24401092965622417	0.0
31	0.0	0.0	0.0	0.5546729928759541	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAT	80	1.8189894E-12	30.0625	1
TGCGCTA	25	0.0054949378	29.599998	10
ATATAGA	25	0.0054949378	29.599998	1
GTACGAT	25	0.0054949378	29.599998	20
TATATCT	95	2.8012437E-10	23.368422	5
ATTAGGT	40	0.001930459	23.125	19
CTCTGTA	40	0.001930459	23.125	2
ATCGATA	40	0.001930459	23.125	23
CTATATC	100	5.329639E-10	22.199999	4
CATTGCG	45	0.0038243788	20.555557	29
TATCTAT	110	1.7516868E-9	20.181818	7
ACTATAT	120	2.382876E-10	20.041668	3
CGGGAGT	260	0.0	19.923077	4
GACTCCT	65	6.897347E-5	19.923077	7
GGGTTAG	85	1.2439268E-6	19.588236	7
GGGAGTG	270	0.0	19.185186	5
GTACTGG	135	5.638867E-11	19.185186	1
ATATAGT	165	0.0	19.060604	1
ATCTATT	120	5.164111E-9	18.5	8
AATTATA	110	3.8453436E-8	18.5	28
>>END_MODULE