FastQCFastQC Report
Fri 10 Feb 2017
ERR1630124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225212
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG70490.3167788057946838No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG61330.27561418867056264No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT59510.26743519269175253No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC58700.2637950900857986No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG56260.25282984272959164No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA44910.2018234667078912No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC42790.19229628457872777No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC40900.1838027118315019No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG40460.18182537214431704No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT39390.17701684154139022No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT39290.17656744615793912No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG38770.17423059016399337No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC38690.17387107385723247No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA38530.1731520412437107No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA37340.16780423618064255No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC36600.16447871034310438No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC35990.16173739850405264No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA35820.16097342635218578No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG35100.15773777959133783No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG34900.1568389888244356No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA34630.15562562128911764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.14987336038094348No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA31480.1414696667104078No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG29070.13063923796923618No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC28350.12740359120838823No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC28200.12672949813321158No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA27630.12416794444754027No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG27610.12407806537085006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27110.12183108845359454No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT27070.12165133030021409No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC26760.12025820461151566No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG26740.12016832553482545No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG26710.12003350691979012No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC26420.11873026030778192No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG25890.11634846477549105No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT25500.11459582278003175No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA25460.1144160646266513No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC25440.11432618554996109No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24890.11185451094098No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG24840.11162981324925444No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC24090.10825934787337117No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA23710.10655164541625696No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC23370.10502370111252321No Hit
GATCTTCAGGTTCCAGGGCATCATTCTCCGTCTGGTTGGGTTC23050.10358563588547967No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA22970.10322611957871879No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC22790.1024172078885068No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC22520.10120384035318883No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC22280.10012529143290617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.020.1281
TATATCG609.240519E-418.55
TACGTTA803.384346E-416.187519
TATCCGC803.384346E-416.187519
ATTAGAC1151.2433975E-616.0869563
TCGACCG1151.2433975E-616.08695633
TCTAGCG1302.5919508E-715.65384628
CTTATAC9500.015.38421137
TCTTATA15600.014.58653937
CTAGCGG1406.001628E-714.53571429
GCTATAC1304.448175E-614.2307693
TAATACT2252.7284841E-1113.97777754
TTACACT2405.456968E-1213.8750014
CTAATAC3350.013.805973
AATGTCG950.001245714413.6315795
TTAAGAC1652.5980626E-713.4545463
TAGGACC3450.013.4057974
CCGGTCC2102.044544E-913.2142859
CGCAATA1551.8895807E-613.12903236
TAGCGTA850.00940834613.0588247
Produced by FastQC (version 0.11.5)