FastQCFastQC Report
Fri 10 Feb 2017
ERR1630123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310230
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47031.515972020758792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35131.1323856493569289No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27020.8709667021242304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.5141346742739258No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.17084098894368693No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5100.16439415917222705No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.14440898688070142No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4480.14440898688070142No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3480.11217483802340199No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.111530155046256No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.10024820294620121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACC200.001839865237.015
CATCTAG200.001839865237.01
TAACGGA200.001839865237.028
CTAACGG200.001839865237.027
GCTAACG200.001839865237.026
TCAAGAC200.001839865237.03
CCTTAAA303.593476E-430.83333219
ATCTAGG250.005490234629.62
CTTATAT250.005490234629.63
AGCTAAC250.005490234629.625
AAGCTTT250.005490234629.637
TCTGATT453.995865E-628.77777720
CATGGAA358.854744E-426.428579
TAGCACA358.854744E-426.428574
TCTAGAC451.320172E-424.66666616
CCGTATA601.3321533E-624.6666662
AATCTAG451.320172E-424.66666614
AGACAAA451.320172E-424.66666619
CGGTCAC451.320172E-424.66666630
TATACAT551.8968589E-523.5454545
Produced by FastQC (version 0.11.5)