##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630123.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	310230
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.706775618089804	31.0	31.0	34.0	30.0	34.0
2	31.91004738419882	33.0	31.0	34.0	30.0	34.0
3	31.9796473584115	33.0	31.0	34.0	30.0	34.0
4	35.66878445024659	37.0	35.0	37.0	33.0	37.0
5	35.62408535602617	37.0	35.0	37.0	33.0	37.0
6	35.70694001224898	37.0	35.0	37.0	33.0	37.0
7	35.69063275634207	37.0	35.0	37.0	33.0	37.0
8	35.71573671147213	37.0	35.0	37.0	33.0	37.0
9	37.41429584501821	39.0	37.0	39.0	34.0	39.0
10	37.30333301099184	39.0	37.0	39.0	34.0	39.0
11	37.37716532894949	39.0	37.0	39.0	34.0	39.0
12	37.29696354317764	39.0	37.0	39.0	34.0	39.0
13	37.34032814363537	39.0	37.0	39.0	34.0	39.0
14	38.451403797182735	40.0	38.0	41.0	34.0	41.0
15	38.449237662379524	40.0	38.0	41.0	34.0	41.0
16	38.37272346323695	40.0	38.0	41.0	34.0	41.0
17	38.42550688199078	40.0	38.0	41.0	34.0	41.0
18	38.43077716532895	40.0	38.0	41.0	34.0	41.0
19	38.49449763079006	40.0	38.0	41.0	34.0	41.0
20	38.4790381329981	40.0	38.0	41.0	34.0	41.0
21	38.44530509621893	40.0	38.0	41.0	34.0	41.0
22	38.39644457338104	40.0	38.0	41.0	34.0	41.0
23	38.338078200045125	40.0	38.0	41.0	34.0	41.0
24	38.30695290590852	40.0	38.0	41.0	34.0	41.0
25	38.28247751668117	40.0	38.0	41.0	34.0	41.0
26	38.05164877671405	40.0	37.0	41.0	33.0	41.0
27	37.91684234277794	40.0	37.0	41.0	33.0	41.0
28	37.82953292718306	40.0	37.0	41.0	33.0	41.0
29	37.74027334558231	40.0	37.0	41.0	33.0	41.0
30	37.60085420494472	40.0	37.0	41.0	33.0	41.0
31	37.440843890017085	39.0	36.0	41.0	32.0	41.0
32	37.29569674112755	39.0	36.0	41.0	32.0	41.0
33	37.163527060567965	39.0	35.0	41.0	32.0	41.0
34	37.092022048157816	39.0	35.0	41.0	31.0	41.0
35	36.90023530928666	39.0	35.0	41.0	31.0	41.0
36	36.75120394545982	39.0	35.0	41.0	31.0	41.0
37	36.631128517551495	39.0	35.0	41.0	30.0	41.0
38	36.477481223608294	39.0	35.0	41.0	30.0	41.0
39	36.32946201205557	39.0	35.0	41.0	30.0	41.0
40	36.12498468877929	39.0	35.0	41.0	30.0	41.0
41	35.91064693936757	39.0	35.0	40.0	28.0	41.0
42	35.76817844824807	39.0	35.0	40.0	27.0	41.0
43	34.6023273055475	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	2.0
13	0.0
14	1.0
15	1.0
16	3.0
17	3.0
18	13.0
19	17.0
20	47.0
21	109.0
22	213.0
23	346.0
24	620.0
25	1022.0
26	1550.0
27	2283.0
28	3231.0
29	4322.0
30	5720.0
31	7356.0
32	8985.0
33	11967.0
34	15723.0
35	20190.0
36	28067.0
37	44567.0
38	70221.0
39	83644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9852045256745	20.309770170518647	13.715952680269478	22.989072623537375
2	18.772201270025466	22.909131934371274	34.22621925668053	24.092447538922734
3	19.213809109370466	26.532894948908876	30.690777810011927	23.56251813170873
4	13.58379266995455	17.450923508364763	36.16993843277568	32.795345388905005
5	12.691551429584502	39.44202688328014	34.36095799890404	13.505463688231313
6	30.15407923153789	37.130193727234634	17.108596847500245	15.607130193727233
7	26.993520936079683	31.878606195403407	23.26306288882442	17.864809979692488
8	28.419559681526607	32.74119201882474	19.793056764336136	19.04619153531251
9	27.10634045708023	13.551880862585824	21.391225864681044	37.950552815652905
10	18.853108983657286	27.93636979015569	31.45666118686136	21.753860039325662
11	32.69832060084453	24.869612867872224	20.99281178480482	21.43925474647842
12	20.566676336911325	29.93972214163685	28.15427263643103	21.33932888502079
13	30.69303420043194	20.454823840376495	25.88885665474003	22.963285304451535
14	23.070302678657768	21.766109015891434	27.499919414627854	27.66366889082294
15	25.994584662991976	27.881249395609707	23.822325371498565	22.301840569899753
16	22.633529961641365	25.998775102343423	27.253650517358093	24.114044418657123
17	21.0205331528221	28.20552493311414	27.67495084292299	23.098991071140766
18	20.566676336911325	25.71575927537633	29.368855365374074	24.348709022338266
19	21.708732230925442	26.179608677432874	31.393482255101052	20.718176836540632
20	21.993037423846822	25.95493665989749	31.21006994810302	20.84195596815266
21	22.773426167682043	26.105470135061083	29.64349031363827	21.477613383618603
22	23.571221351900203	25.640976050027398	29.304387067659476	21.48341553041292
23	22.412081358991713	26.453276601231345	29.609966798826676	21.524675240950263
24	20.78812493956097	27.480256583824904	29.06907778100119	22.662540695612932
25	21.94952132288947	26.626696322083614	30.35522032040744	21.068562034619475
26	21.690036424588207	26.866840731070496	29.933919994842533	21.50920284949876
27	20.759436547077975	27.670760403571542	29.359185120716884	22.210617928633596
28	21.286787222383392	27.174999194146277	31.095316378171034	20.442897205299293
29	21.813493214711666	26.990619862682525	30.490603745608098	20.70528317699771
30	21.17590175031428	28.24742932662863	29.517777133094803	21.058891789962285
31	21.62266705347645	27.495406633787834	30.096057763594754	20.78586854914096
32	20.32846597685588	27.874157882861102	30.057376784965996	21.73999935531702
33	20.382619346936146	28.131708732230926	30.2088772845953	21.276794636237632
34	21.49534216549012	27.206911001515007	29.754053444218805	21.54369338877607
35	21.293878735131997	27.933468716758536	29.960351996905523	20.812300551203947
36	21.064049253779455	28.045643554781936	28.499822712181285	22.390484479257324
37	20.74267478967218	27.042194500854205	30.118299326306293	22.096831383167327
38	20.692711858943362	27.74941172678335	29.939399800148276	21.618476614125004
39	19.865905940753635	27.47864487638204	30.896109338232925	21.759339844631402
40	20.532830480611157	26.88489185443058	31.560455146181866	21.021822518776393
41	19.88170067369371	26.951616542565194	31.070496083550914	22.09618670019018
42	19.023949972600974	27.379686039390126	31.170744286497115	22.42561970151178
43	19.67153402314412	26.435870160848403	31.4943751410244	22.398220674983076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	56.0
2	81.0
3	235.0
4	389.0
5	389.0
6	584.0
7	779.0
8	782.5
9	786.0
10	1032.5
11	1279.0
12	1279.0
13	2528.5
14	3778.0
15	6406.0
16	9034.0
17	7381.5
18	5729.0
19	5729.0
20	6069.0
21	6409.0
22	4413.0
23	2417.0
24	2599.5
25	2782.0
26	2782.0
27	3224.5
28	3667.0
29	4633.5
30	5600.0
31	6714.0
32	7828.0
33	7828.0
34	9962.0
35	12096.0
36	13670.5
37	15245.0
38	16316.0
39	17387.0
40	17387.0
41	19921.0
42	22455.0
43	24318.0
44	26181.0
45	27662.0
46	29143.0
47	29143.0
48	30000.5
49	30858.0
50	28470.5
51	26083.0
52	23566.0
53	21049.0
54	21049.0
55	19219.0
56	17389.0
57	14576.0
58	11763.0
59	10308.0
60	8853.0
61	8853.0
62	7854.0
63	6855.0
64	5675.5
65	4496.0
66	3638.0
67	2780.0
68	2780.0
69	2249.0
70	1718.0
71	1552.0
72	1386.0
73	1405.5
74	1425.0
75	1425.0
76	1331.0
77	1237.0
78	963.5
79	690.0
80	472.0
81	254.0
82	254.0
83	190.5
84	127.0
85	103.0
86	79.0
87	72.5
88	66.0
89	66.0
90	42.0
91	18.0
92	12.5
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	310230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.6950646472544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.82012216970489	22.827734080177013
2	11.975364108422548	7.830706073985703
3	4.8620551084789945	4.768956182707026
4	2.6721070125210398	3.4945884607502884
5	1.7823911736534617	2.913769732464778
6	1.17359866577035	2.302253054837586
7	0.9906612042519941	2.2672812481582088
8	0.7697367572581776	2.0133274431939263
9	0.6147897476448424	1.8090531489345603
>10	4.785363450351976	30.83528641057557
>50	0.4548444474430978	9.909754557456145
>100	0.0929682057411167	4.597642393131121
>500	0.0019993162524971336	0.33991242545364087
>1k	0.003998632504994267	4.089734788174431
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4703	1.515972020758792	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3513	1.1323856493569289	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2702	0.8709667021242304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1595	0.5141346742739258	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	530	0.17084098894368693	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	510	0.16439415917222705	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	448	0.14440898688070142	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	448	0.14440898688070142	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	348	0.11217483802340199	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	346	0.111530155046256	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.10024820294620121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	6.446829771459884E-4	0.0
17	0.0	0.0	0.0	0.0019340489314379654	0.0
18	0.0	0.0	0.0	0.0032234148857299425	0.0
19	0.0	0.0	0.0	0.005157463817167907	0.0
20	0.0	0.0	0.0	0.005802146794313896	0.0
21	0.0	0.0	0.0	0.00838087870289785	0.0
22	0.0	0.0	0.0	0.011926635077200787	0.0
23	0.0	0.0	0.0	0.019985172291525643	0.0
24	0.0	0.0	0.0	0.03126712439158044	0.0
25	0.0	0.0	0.0	0.035457563743029365	0.0
26	0.0	0.0	0.0	0.039325661605905296	0.0
27	0.0	0.0	0.0	0.05092995519453309	0.0
28	0.0	0.0	0.0	0.0731715179060697	0.0
29	0.0	0.0	0.0	0.11378654546626696	0.0
30	0.0	0.0	0.0	0.20823260161815427	0.0
31	0.0	0.0	0.0	0.48931437965380525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACC	20	0.0018398652	37.0	15
CATCTAG	20	0.0018398652	37.0	1
TAACGGA	20	0.0018398652	37.0	28
CTAACGG	20	0.0018398652	37.0	27
GCTAACG	20	0.0018398652	37.0	26
TCAAGAC	20	0.0018398652	37.0	3
CCTTAAA	30	3.593476E-4	30.833332	19
ATCTAGG	25	0.0054902346	29.6	2
CTTATAT	25	0.0054902346	29.6	3
AGCTAAC	25	0.0054902346	29.6	25
AAGCTTT	25	0.0054902346	29.6	37
TCTGATT	45	3.995865E-6	28.777777	20
CATGGAA	35	8.854744E-4	26.42857	9
TAGCACA	35	8.854744E-4	26.42857	4
TCTAGAC	45	1.320172E-4	24.666666	16
CCGTATA	60	1.3321533E-6	24.666666	2
AATCTAG	45	1.320172E-4	24.666666	14
AGACAAA	45	1.320172E-4	24.666666	19
CGGTCAC	45	1.320172E-4	24.666666	30
TATACAT	55	1.8968589E-5	23.545454	5
>>END_MODULE