FastQCFastQC Report
Fri 10 Feb 2017
ERR1630122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529777
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81040.3203444414270507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64990.2569001141207308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60220.23804469722034788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.13416202297672877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG5500.020.1818181
TCTAGAC5650.018.008853
GGTATCA28700.016.5017411
ACCGTCG1951.8189894E-1216.1282068
CTAGACA7000.016.1214284
CGAATTA2300.016.08695615
TATACTG4250.015.6705875
CTCTAGT5750.015.44347927
TAGACAT7500.015.2933345
TACCGTC1954.1836756E-1115.1794877
CTTATAC15400.015.01623337
TCTAGTT6400.014.45312528
ATACCGT2450.014.3469386
CCGTCGT1854.9058144E-914.09
CGAATCG1356.573966E-613.70370316
GACGGAC2051.4260877E-913.5365857
ACATGTA7400.013.58
GTATTAG5100.013.4215691
TTCTAGA9200.013.2717392
TACACCG1409.5688665E-613.2142855
Produced by FastQC (version 0.11.5)