##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630122.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2529777
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875589034132258	33.0	31.0	34.0	30.0	34.0
2	32.08467979588715	33.0	31.0	34.0	30.0	34.0
3	32.22670575311579	34.0	31.0	34.0	30.0	34.0
4	35.85526392247222	37.0	35.0	37.0	35.0	37.0
5	35.77900067871595	37.0	35.0	37.0	35.0	37.0
6	35.83021467900135	37.0	35.0	37.0	35.0	37.0
7	35.80493735218559	37.0	35.0	37.0	35.0	37.0
8	35.8058176669327	37.0	35.0	37.0	35.0	37.0
9	37.55607786773301	39.0	37.0	39.0	35.0	39.0
10	37.45013888575949	39.0	37.0	39.0	34.0	39.0
11	37.51645817002843	39.0	37.0	39.0	35.0	39.0
12	37.44154563821238	39.0	37.0	39.0	34.0	39.0
13	37.45011951646331	39.0	37.0	39.0	35.0	39.0
14	38.701761459606914	40.0	38.0	41.0	35.0	41.0
15	38.708633606835704	40.0	38.0	41.0	35.0	41.0
16	38.67831196188439	40.0	38.0	41.0	34.0	41.0
17	38.65047235388732	40.0	38.0	41.0	34.0	41.0
18	38.61539732553502	40.0	38.0	41.0	34.0	41.0
19	38.65219068716333	40.0	38.0	41.0	34.0	41.0
20	38.62870956610009	40.0	38.0	41.0	34.0	41.0
21	38.57246864051653	40.0	38.0	41.0	34.0	41.0
22	38.53664097665526	40.0	38.0	41.0	34.0	41.0
23	38.483315723085475	40.0	38.0	41.0	34.0	41.0
24	38.460061894783614	40.0	38.0	41.0	34.0	41.0
25	38.44414468152726	40.0	38.0	41.0	34.0	41.0
26	38.28909781375987	40.0	38.0	41.0	34.0	41.0
27	38.17424263087221	40.0	38.0	41.0	33.0	41.0
28	38.1082166530884	40.0	38.0	41.0	33.0	41.0
29	38.05289478084432	40.0	38.0	41.0	33.0	41.0
30	37.99285786849987	40.0	37.0	41.0	33.0	41.0
31	37.93645961679626	40.0	37.0	41.0	33.0	41.0
32	37.84971876967812	40.0	37.0	41.0	33.0	41.0
33	37.766303907419505	40.0	37.0	41.0	33.0	41.0
34	37.700671640227576	40.0	37.0	41.0	33.0	41.0
35	37.59470143020511	40.0	37.0	41.0	32.0	41.0
36	37.51153125354527	40.0	37.0	41.0	32.0	41.0
37	37.452700376357285	40.0	37.0	41.0	32.0	41.0
38	37.35504631435894	40.0	37.0	41.0	31.0	41.0
39	37.2778426715082	40.0	36.0	41.0	31.0	41.0
40	37.184660940470245	40.0	36.0	41.0	31.0	41.0
41	37.06638846032674	39.0	36.0	41.0	31.0	41.0
42	37.03007537818551	39.0	36.0	41.0	31.0	41.0
43	35.857042735387346	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	3.0
11	13.0
12	2.0
13	1.0
14	3.0
15	3.0
16	10.0
17	32.0
18	61.0
19	126.0
20	293.0
21	598.0
22	1173.0
23	2153.0
24	3706.0
25	5865.0
26	9182.0
27	13689.0
28	20010.0
29	27907.0
30	37575.0
31	49671.0
32	63891.0
33	82603.0
34	108551.0
35	145094.0
36	204090.0
37	311577.0
38	580442.0
39	861447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17786785159324	19.514803083433836	12.477502957770586	25.829826107202337
2	17.611196560013	21.111465556054938	35.809282794491374	25.46805508944069
3	19.113542418956296	23.362454477212815	30.167599752863595	27.3564033509673
4	13.963681383774142	15.767634854771783	35.66705681963272	34.60162694182136
5	14.31051037304869	36.663547814688805	34.48039095936124	14.545550852901263
6	33.83242870814305	36.265054192523685	15.930336942742384	13.972180156590877
7	28.874956172026227	30.958934325041298	21.75211490973315	18.41399459319932
8	26.741092199035727	34.83322838337134	20.05081870852648	18.37486070906645
9	26.454031323709557	14.528830011499036	20.02239723106029	38.994741433731114
10	16.31641840367748	27.161089692885977	33.7859819264702	22.73650997696635
11	35.37805901468786	22.240379290348518	22.130883473128264	20.250678221835365
12	21.06924839620251	25.727643187522066	30.52917312474578	22.67393529152965
13	29.821679934634552	20.2923815024012	25.367571924323762	24.518366638640483
14	22.193497687740855	20.514891233496073	26.21709344341418	31.074517635348887
15	24.421362041002034	28.06800757537127	22.968902002034174	24.541728381592527
16	24.137621616450776	27.070449292566103	24.51429513352363	24.27763395745949
17	22.805132626314492	26.979848421422126	26.44252833352505	23.77249061873833
18	23.24173237403929	26.069886792393167	27.11677748671128	23.571603346856264
19	23.729917696302877	25.895563126710382	27.15595090002004	23.218568276966707
20	23.912028609636344	25.916948410867835	26.929883543094906	23.241139436400914
21	24.238816306733753	25.7289081211506	26.592818260265627	23.439457311850017
22	24.078090677557746	25.840617572220793	26.722513486366584	23.358778263854877
23	23.7302734588859	25.844372843930515	26.757694452910275	23.66765924427331
24	23.532508991899288	26.082931420437454	26.59605965268875	23.788499934974507
25	24.07002672567582	25.659415829932836	26.717888572787245	23.552668871604098
26	23.80435113450711	25.9816181426268	26.596494473623565	23.61753624924252
27	23.53365533800015	26.008972332343916	26.62297902147106	23.834393308184872
28	23.589510063535243	25.905208245627975	26.95431257379603	23.55096911704075
29	23.241060378049134	26.096964277879035	27.04953835851935	23.61243698555248
30	23.31952579219433	26.50751429869115	26.718481510425622	23.454478398688895
31	23.681533985011328	26.15270041588646	26.557162943611235	23.60860265549098
32	23.216394172292656	26.213021938297327	26.80580145997058	23.764782429439432
33	23.128323168405753	25.96782246024057	27.29442160316897	23.609432768184703
34	23.705370078074075	25.544069694680598	26.92336122907276	23.827198998172566
35	23.34071343047233	25.81915322971155	27.338773338519562	23.501360001296558
36	23.534050629759065	25.592492935148037	26.984987214288058	23.888469220804836
37	23.37099277920544	25.12553478033834	27.449850322775486	24.053622117680728
38	22.994556437187942	25.285193121765275	27.857079892812685	23.863170548234095
39	22.880119472981214	25.173878962454005	28.142322426047826	23.803679138516955
40	22.759832190742504	25.029716057976653	28.41218810986107	23.79826364141978
41	22.476368470422493	24.8725480546309	28.614498432075237	24.036585042871366
42	22.353116499991895	25.08849594252774	28.581096278446676	23.977291279033686
43	22.032930175268415	24.727713154163393	28.809614444277106	24.429742226291093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	536.0
1	531.0
2	526.0
3	1164.5
4	1803.0
5	1803.0
6	2269.0
7	2735.0
8	2559.5
9	2384.0
10	3389.0
11	4394.0
12	4394.0
13	7271.0
14	10148.0
15	14753.0
16	19358.0
17	19332.0
18	19306.0
19	19306.0
20	23378.5
21	27451.0
22	28004.5
23	28558.0
24	33900.5
25	39243.0
26	39243.0
27	45032.0
28	50821.0
29	58361.0
30	65901.0
31	73507.5
32	81114.0
33	81114.0
34	91639.5
35	102165.0
36	112058.0
37	121951.0
38	129894.0
39	137837.0
40	137837.0
41	146735.0
42	155633.0
43	159722.5
44	163812.0
45	167704.0
46	171596.0
47	171596.0
48	173760.0
49	175924.0
50	177427.5
51	178931.0
52	176047.5
53	173164.0
54	173164.0
55	166129.5
56	159095.0
57	151413.5
58	143732.0
59	137321.5
60	130911.0
61	130911.0
62	120173.0
63	109435.0
64	96977.5
65	84520.0
66	72762.0
67	61004.0
68	61004.0
69	51615.5
70	42227.0
71	35106.5
72	27986.0
73	22193.0
74	16400.0
75	16400.0
76	12582.5
77	8765.0
78	6838.5
79	4912.0
80	3841.0
81	2770.0
82	2770.0
83	2013.5
84	1257.0
85	981.0
86	705.0
87	573.0
88	441.0
89	441.0
90	343.0
91	245.0
92	151.0
93	57.0
94	37.5
95	18.0
96	18.0
97	10.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2529777.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.209094852806174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79844533906211	47.03204368700742
2	11.073974311822381	12.892120422288162
3	3.344365170065762	5.840174082203376
4	1.4685118511581294	3.4192298254613416
5	0.8002306510651589	2.3290350935987343
6	0.5043980215289292	1.7616331367247124
7	0.33914788564373216	1.3819044011195265
8	0.24340815346320946	1.133485463430909
9	0.1873451039624709	0.9814670034084271
>10	1.0850043006932135	11.787590679990744
>50	0.09696588340467303	3.973739322600351
>100	0.05589607948020643	5.657490396385747
>500	0.0017643666148393624	0.6762148775779969
>1k	3.393012720832165E-4	0.3191667860747751
>5k	2.035807632499299E-4	0.814704822127752
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8104	0.3203444414270507	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6499	0.2569001141207308	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6022	0.23804469722034788	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3394	0.13416202297672877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1858752767536427E-4	0.0
2	0.0	0.0	0.0	1.1858752767536427E-4	0.0
3	0.0	0.0	0.0	1.1858752767536427E-4	0.0
4	0.0	0.0	0.0	1.1858752767536427E-4	0.0
5	0.0	0.0	0.0	1.1858752767536427E-4	0.0
6	0.0	0.0	0.0	1.1858752767536427E-4	0.0
7	0.0	0.0	0.0	1.1858752767536427E-4	0.0
8	0.0	0.0	0.0	1.1858752767536427E-4	0.0
9	0.0	0.0	0.0	1.1858752767536427E-4	0.0
10	0.0	0.0	0.0	1.9764587945894044E-4	0.0
11	0.0	0.0	0.0	1.9764587945894044E-4	0.0
12	0.0	0.0	0.0	1.9764587945894044E-4	0.0
13	0.0	0.0	0.0	2.3717505535072854E-4	0.0
14	0.0	0.0	0.0	2.7670423124251663E-4	0.0
15	0.0	0.0	0.0	2.7670423124251663E-4	3.952917589178809E-5
16	0.0	0.0	0.0	5.138792865932452E-4	3.952917589178809E-5
17	0.0	0.0	0.0	7.510543419439737E-4	3.952917589178809E-5
18	0.0	0.0	0.0	9.091710455111261E-4	3.952917589178809E-5
19	0.0	0.0	0.0	9.882293972947023E-4	3.952917589178809E-5
20	0.0	0.0	0.0	0.0011068169249700665	3.952917589178809E-5
21	0.0	0.0	0.0	0.0014230503321043713	3.952917589178809E-5
22	0.0	0.0	0.0	0.002292692201723709	3.952917589178809E-5
23	0.0	3.952917589178809E-5	0.0	0.003359979950801988	3.952917589178809E-5
24	0.0	3.952917589178809E-5	0.0	0.005771259680201061	3.952917589178809E-5
25	0.0	3.952917589178809E-5	0.0	0.0071943100123054325	3.952917589178809E-5
26	0.0	3.952917589178809E-5	0.0	0.009684648093488082	3.952917589178809E-5
27	0.0	3.952917589178809E-5	0.0	0.025773022681445833	3.952917589178809E-5
28	0.0	7.905835178357617E-5	0.0	0.0700456996802485	3.952917589178809E-5
29	0.0	1.5811670356715235E-4	0.0	0.13123686396073647	3.952917589178809E-5
30	0.0	1.5811670356715235E-4	0.0	0.22029609724493504	3.952917589178809E-5
31	0.0	1.5811670356715235E-4	0.0	0.4698042554739015	3.952917589178809E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAG	550	0.0	20.181818	1
TCTAGAC	565	0.0	18.00885	3
GGTATCA	2870	0.0	16.501741	1
ACCGTCG	195	1.8189894E-12	16.128206	8
CTAGACA	700	0.0	16.121428	4
CGAATTA	230	0.0	16.086956	15
TATACTG	425	0.0	15.670587	5
CTCTAGT	575	0.0	15.443479	27
TAGACAT	750	0.0	15.293334	5
TACCGTC	195	4.1836756E-11	15.179487	7
CTTATAC	1540	0.0	15.016233	37
TCTAGTT	640	0.0	14.453125	28
ATACCGT	245	0.0	14.346938	6
CCGTCGT	185	4.9058144E-9	14.0	9
CGAATCG	135	6.573966E-6	13.703703	16
GACGGAC	205	1.4260877E-9	13.536585	7
ACATGTA	740	0.0	13.5	8
GTATTAG	510	0.0	13.421569	1
TTCTAGA	920	0.0	13.271739	2
TACACCG	140	9.5688665E-6	13.214285	5
>>END_MODULE