FastQCFastQC Report
Fri 10 Feb 2017
ERR1630121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738575
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT302924.1014115018786175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197882.6792133500321564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT180112.4386149003147954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100061.3547710117455913No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA45510.6161865755001185No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34840.4717191889787767No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA33400.45222218461226005No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30390.4114680296516941No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.334698574958535No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22750.3080255898182311No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.3066716311816674No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA17860.24181701249026843No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA14740.19957350302948246No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13670.18508614561825137No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.1833259993907186No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA12330.16694309988829842No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA11890.16098568188741832No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.15827776461429102No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA11690.15827776461429102No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9820.13295873811055073No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9200.12456419456385608No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA9080.1229394441999797No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8870.12009613106319603No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA8150.11034762887993771No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8070.10926446197068679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC250.005495087729.59999822
GCTTAGG250.005495087729.59999813
AGACCGT250.005495087729.59999821
CATCCAG250.005495087729.59999816
AAGTTCC454.0057075E-628.7777774
AAGTGTG358.86638E-426.4285727
TAAGTTC509.085346E-625.8999983
CGCCGAA509.085346E-625.89999833
AACAGAG751.3720637E-824.6666662
GGTCAGA851.9736035E-923.9411771
CGTTTTG551.9014657E-523.54545414
TCTTCGC400.00193053723.12534
CTAGTAC400.00193053723.1253
CTGTGCG400.00193053723.1259
ACAGAGG753.7370228E-722.23
GCGCTAT502.7008355E-422.19999919
GGCAGGG603.723966E-521.58333213
GTCGAGA603.723966E-521.58333211
AGAGCGG957.1340764E-921.4210535
ATTAGGT450.00382453320.55555519
Produced by FastQC (version 0.11.5)