##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630121.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	738575
Sequences flagged as poor quality	0
Sequence length	43
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718153200419728	31.0	31.0	34.0	30.0	34.0
2	31.82407744643401	33.0	31.0	34.0	30.0	34.0
3	31.864229089801306	33.0	31.0	34.0	30.0	34.0
4	35.598206004806556	37.0	35.0	37.0	33.0	37.0
5	35.57734691805165	37.0	35.0	37.0	33.0	37.0
6	35.70512676437735	37.0	35.0	37.0	33.0	37.0
7	35.654119080662085	37.0	35.0	37.0	33.0	37.0
8	35.74082388383035	37.0	35.0	37.0	33.0	37.0
9	37.36969705175507	39.0	37.0	39.0	34.0	39.0
10	37.26143181125816	39.0	37.0	39.0	34.0	39.0
11	37.36293538232407	39.0	37.0	39.0	34.0	39.0
12	37.32199438107166	39.0	37.0	39.0	34.0	39.0
13	37.3669728869783	39.0	37.0	39.0	34.0	39.0
14	38.33950783603561	40.0	38.0	41.0	34.0	41.0
15	38.31642148732356	40.0	38.0	41.0	34.0	41.0
16	38.2465653454287	40.0	38.0	41.0	33.0	41.0
17	38.4219625630437	40.0	38.0	41.0	34.0	41.0
18	38.52044139051552	40.0	38.0	41.0	34.0	41.0
19	38.615292962799984	40.0	38.0	41.0	34.0	41.0
20	38.651355651084856	40.0	38.0	41.0	34.0	41.0
21	38.619895068205665	40.0	38.0	41.0	34.0	41.0
22	38.55968858951359	40.0	38.0	41.0	34.0	41.0
23	38.485502487899	40.0	38.0	41.0	34.0	41.0
24	38.42831939884236	40.0	38.0	41.0	34.0	41.0
25	38.384012456419455	40.0	38.0	41.0	34.0	41.0
26	38.105277053786004	40.0	37.0	41.0	34.0	41.0
27	37.9013776529127	40.0	37.0	41.0	33.0	41.0
28	37.73686220085976	40.0	36.0	41.0	33.0	41.0
29	37.53516839860542	39.0	36.0	41.0	33.0	41.0
30	37.23976034932133	39.0	35.0	41.0	33.0	41.0
31	36.912106421148835	39.0	35.0	40.0	32.0	41.0
32	36.623552110483026	38.0	35.0	40.0	32.0	41.0
33	36.3918275056697	38.0	35.0	40.0	31.0	41.0
34	36.22993602545442	38.0	35.0	40.0	31.0	41.0
35	35.94195037741597	38.0	35.0	40.0	30.0	41.0
36	35.67115594218597	38.0	35.0	40.0	30.0	41.0
37	35.40020309379548	38.0	35.0	40.0	28.0	41.0
38	35.104422705886336	37.0	35.0	40.0	26.0	41.0
39	34.777349625968924	37.0	34.0	40.0	24.0	41.0
40	34.382172426632366	37.0	34.0	40.0	22.0	41.0
41	33.94581999119927	37.0	33.0	40.0	18.0	41.0
42	33.53609992214738	37.0	33.0	40.0	15.0	41.0
43	32.42713603899401	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	19.0
11	21.0
12	15.0
13	10.0
14	9.0
15	10.0
16	11.0
17	15.0
18	35.0
19	73.0
20	151.0
21	313.0
22	591.0
23	1057.0
24	1877.0
25	2955.0
26	4775.0
27	6874.0
28	9668.0
29	13144.0
30	16739.0
31	20439.0
32	25455.0
33	32610.0
34	42241.0
35	52773.0
36	75047.0
37	129271.0
38	143461.0
39	158907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.535050604204045	19.67965338658904	15.1395592864638	17.64573672274312
2	16.867887486037304	27.781877263649594	33.86399485495718	21.48624039535592
3	19.876383576481736	30.344108587482655	32.00419727177335	17.775310564262263
4	12.457231831567546	17.636529804014486	34.24987306637782	35.65636529804014
5	10.779541685001522	44.237484344853264	31.485360322242155	13.497613647903057
6	27.39396811427411	37.17672545103747	18.333953897708426	17.095352536979995
7	23.920116440442747	35.477642758013744	22.665944555393832	17.93629624614968
8	31.943404528991636	31.295941508986903	19.46762346410317	17.293030497918288
9	28.300443421453476	12.187523271164066	22.704667772399553	36.80736553498291
10	19.15810851978472	32.30721321463629	27.73448871136987	20.80018955420912
11	29.187150932539012	28.63622516332126	19.50824222320008	22.668381680939646
12	19.792167349287478	35.26994550316488	27.72040754154961	17.217479605998037
13	30.634261923298244	21.836644890498597	27.08282841959178	20.44626476661138
14	22.23619808414853	24.26997935213079	31.51866770470162	21.975154859019057
15	24.6540974173239	29.815387739904548	27.45354229428291	18.076972548488644
16	18.170801882002504	28.520190908167752	30.47002674068307	22.838980469146666
17	17.308736418102427	31.43418068578005	31.432826727143485	19.82425616897404
18	15.808685644653556	26.935111532342688	35.45408387773754	21.802118945266223
19	17.308194834647804	28.974308634871203	38.09863588667366	15.61886064380733
20	17.87739904545916	28.071759807737873	38.19788105473378	15.852960092069187
21	19.515824391564838	27.776326033239684	36.28920556476999	16.41864401042548
22	19.43038960159767	28.560132687946382	35.22215076329418	16.787326947161766
23	17.491250042311208	29.755271976441122	36.07135362014691	16.68212436110077
24	15.985648038452425	31.483329384287313	35.21348542802018	17.317537149240092
25	16.26645905967573	30.690045019124668	36.094912500423106	16.948583420776494
26	16.935991605456454	30.865788850150626	35.82588091933792	16.372338625055004
27	15.8085502487899	31.7011813289104	35.082963815455436	17.407304606844264
28	15.807060894289679	31.95870426158481	37.28558372541719	14.948651118708323
29	16.852452357580475	31.6565006939038	36.28893477304268	15.20211217547304
30	15.638086856446535	33.09846664184409	36.30477608909048	14.958670412618893
31	16.068645702873777	32.63080932877501	35.99323020681718	15.307314761534036
32	14.200182784415935	33.02846698033375	36.241681616626614	16.529668618623703
33	14.23646887587584	33.32972277696916	36.845411772670346	15.58839657448465
34	15.247605185661579	32.79599228243577	35.50282638865382	16.453576143248824
35	14.932945198524186	33.92451680601158	35.14064245337305	16.001895542091187
36	15.279017026029853	33.53268117659006	34.01117015875165	17.17713163862844
37	14.726331110584573	33.186067765629765	34.851301492739395	17.236299631046272
38	14.857800494194903	32.863690214263954	35.08837965000169	17.19012964153945
39	14.2760044680635	32.77446434011441	35.86446874047998	17.08506245134211
40	14.468401990319196	33.08722878516061	36.01286260704735	16.431506617472834
41	14.185560031141048	32.97661036455336	35.825745523474254	17.01208408083133
42	13.750939308804117	33.14057475544122	35.6223809362624	17.486104999492266
43	13.788308567173274	32.45479470602174	35.79974951765224	17.95714720915276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	286.0
2	428.0
3	1539.0
4	2650.0
5	2650.0
6	3959.5
7	5269.0
8	5076.5
9	4884.0
10	6964.5
11	9045.0
12	9045.0
13	17592.0
14	26139.0
15	43171.5
16	60204.0
17	48980.0
18	37756.0
19	37756.0
20	38926.5
21	40097.0
22	27503.5
23	14910.0
24	15007.5
25	15105.0
26	15105.0
27	15206.0
28	15307.0
29	16567.5
30	17828.0
31	19368.0
32	20908.0
33	20908.0
34	24570.0
35	28232.0
36	30726.0
37	33220.0
38	35834.0
39	38448.0
40	38448.0
41	42804.5
42	47161.0
43	50234.0
44	53307.0
45	55623.0
46	57939.0
47	57939.0
48	58899.0
49	59859.0
50	51906.0
51	43953.0
52	37993.5
53	32034.0
54	32034.0
55	28513.5
56	24993.0
57	20874.5
58	16756.0
59	14404.0
60	12052.0
61	12052.0
62	10079.5
63	8107.0
64	6244.0
65	4381.0
66	3483.0
67	2585.0
68	2585.0
69	2039.5
70	1494.0
71	1263.5
72	1033.0
73	932.5
74	832.0
75	832.0
76	689.0
77	546.0
78	478.5
79	411.0
80	313.5
81	216.0
82	216.0
83	183.0
84	150.0
85	119.0
86	88.0
87	81.0
88	74.0
89	74.0
90	50.0
91	26.0
92	14.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	738575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.690597390426948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.24450835172115	16.404437688586818
2	11.722241232334392	5.554137950973927
3	5.080276496260378	3.6106435531486043
4	2.8060334150253508	2.6590643159780156
5	1.860529658239375	2.2038529533148847
6	1.2859595383580031	1.8279089810171252
7	0.9843675628859565	1.6324178931568882
8	0.7044046705464515	1.3350213959881854
9	0.6091204040106639	1.298738362833976
>10	4.76713873783243	22.62239809727547
>50	0.5892228498185585	9.779476216178512
>100	0.3192579059814552	13.556428881452087
>500	0.01604887139583617	2.542908235217641
>1k	0.008597609676340805	4.367903519103213
>5k	0.0	0.0
>10k+	0.0022926959136908816	10.604661955774663
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30292	4.1014115018786175	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19788	2.6792133500321564	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18011	2.4386149003147954	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10006	1.3547710117455913	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4551	0.6161865755001185	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3484	0.4717191889787767	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	3340	0.45222218461226005	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3039	0.4114680296516941	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2472	0.334698574958535	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2275	0.3080255898182311	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2265	0.3066716311816674	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1786	0.24181701249026843	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	1474	0.19957350302948246	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1367	0.18508614561825137	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1354	0.1833259993907186	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1233	0.16694309988829842	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1189	0.16098568188741832	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1169	0.15827776461429102	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	1169	0.15827776461429102	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	982	0.13295873811055073	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	920	0.12456419456385608	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	908	0.1229394441999797	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	887	0.12009613106319603	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	815	0.11034762887993771	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	807	0.10926446197068679	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.707917273127306E-4	0.0
11	0.0	0.0	0.0	5.415834546254612E-4	0.0
12	0.0	0.0	0.0	6.769793182818265E-4	0.0
13	0.0	0.0	0.0	6.769793182818265E-4	0.0
14	0.0	0.0	0.0	9.477710455945571E-4	0.0
15	0.0	0.0	0.0	0.002707917273127306	0.0
16	0.0	0.0	0.0	0.005145042818941881	0.0
17	0.0	0.0	0.0	0.007717564228412822	0.0
18	0.0	0.0	0.0	0.008394543546694649	0.0
19	0.0	0.0	0.0	0.010154689774227397	0.0
20	0.0	0.0	0.0	0.01205023186541651	0.0
21	0.0	0.0	0.0	0.015028940865856547	0.0
22	0.0	0.0	0.0	0.022340317503300275	0.0
23	0.0	0.0	0.0	0.035202924550654976	0.0
24	0.0	0.0	0.0	0.05185661578038791	0.0
25	0.0	0.0	0.0	0.056053887553735235	0.0
26	0.0	0.0	0.0	0.06431303523677352	0.0
27	0.0	0.0	0.0	0.07731103814778459	0.0
28	0.0	0.0	0.0	0.11224317097112683	0.0
29	0.0	0.0	0.0	0.17303591375283486	0.0
30	0.0	0.0	0.0	0.3245438851843076	0.0
31	0.0	0.0	0.0	0.7834004671157296	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTC	25	0.0054950877	29.599998	22
GCTTAGG	25	0.0054950877	29.599998	13
AGACCGT	25	0.0054950877	29.599998	21
CATCCAG	25	0.0054950877	29.599998	16
AAGTTCC	45	4.0057075E-6	28.777777	4
AAGTGTG	35	8.86638E-4	26.428572	7
TAAGTTC	50	9.085346E-6	25.899998	3
CGCCGAA	50	9.085346E-6	25.899998	33
AACAGAG	75	1.3720637E-8	24.666666	2
GGTCAGA	85	1.9736035E-9	23.941177	1
CGTTTTG	55	1.9014657E-5	23.545454	14
TCTTCGC	40	0.001930537	23.125	34
CTAGTAC	40	0.001930537	23.125	3
CTGTGCG	40	0.001930537	23.125	9
ACAGAGG	75	3.7370228E-7	22.2	3
GCGCTAT	50	2.7008355E-4	22.199999	19
GGCAGGG	60	3.723966E-5	21.583332	13
GTCGAGA	60	3.723966E-5	21.583332	11
AGAGCGG	95	7.1340764E-9	21.421053	5
ATTAGGT	45	0.003824533	20.555555	19
>>END_MODULE