FastQCFastQC Report
Fri 10 Feb 2017
ERR1630120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564292
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT213773.788286915284994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139062.4643269796488343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124852.2125069999220264No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70411.2477582528194622No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA32560.5770062308166694No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24060.4263749973418018No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA22950.4067043303821426No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.3728566061542606No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.3368823233361451No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.28159179999007605No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15590.2762754035144925No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA10660.18890928809906926No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA10580.187491582372247No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.1851878105661608No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.16746648898088223No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA9180.16268173215285703No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA8930.1582514017565374No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8130.14407434448831458No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.1414161462505228No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7310.12954286078838614No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7160.12688466255059438No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6120.10845448810190468No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA6100.10810006167019913No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5820.10313809162632112No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5680.10065710660438212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA200.001841142237.08
CGTCCCA200.001841142237.031
CAATGCG603.45608E-1133.91666419
GTACTAG800.032.3751
ACATGGA352.3861687E-531.7142873
CGACGAG658.731149E-1131.30769224
AGCGTTG250.005494002329.59999830
CGTTAAC250.005494002329.5999989
CAGGATC250.005494002329.59999815
CCGACGA250.005494002329.59999824
CGATGTA502.7166243E-729.59999820
AGGATCT250.005494002329.59999816
TCAAGAC250.005494002329.5999983
CGAGTCC754.620233E-1027.13333127
TAGCTGA754.620233E-1027.1333316
GCGATGT556.2522486E-726.9090919
AGCAACC358.863775E-426.42857212
GCAACCG358.863775E-426.42857213
TAGAAAG358.863775E-426.4285727
TAGTTCG358.863775E-426.42857212
Produced by FastQC (version 0.11.5)