FastQCFastQC Report
Fri 10 Feb 2017
ERR1630119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213679
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99970.31107649519444847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT93610.29128609297941704No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG79150.24629093322637388No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG78670.24479731796486207No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74720.2325061090420045No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC68960.21458272590386282No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG68870.21430267304232936No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT67550.2101952310731719No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA49640.15446471162801262No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC47750.14858360153580988No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44260.13772377390523446No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC43320.13479877735144052No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA42910.1335229809822325No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT42800.13318069415146938No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG42610.13258947144378763No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT42220.13137590904380927No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA41900.13038016553613474No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA40940.12739293501311114No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC40170.12499692719776928No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA39740.12365889685933162No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG39260.12216528159781981No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC38740.12054719839784869No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG38720.12048496442861904No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG37270.11597300165946879No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC37060.11531954498255738No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA35460.11034082744418468No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC35300.10984295569034742No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT33080.10293498510585532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38550.019.5317781
CGTTTCG701.2200621E-418.523
AAGACGG4900.017.74495
CGATAGA2050.017.14634137
TCTAGCG3850.014.89610528
TAATACG1001.094335E-414.7999994
TACGACG3550.014.5915495
TTAGACT3300.014.5757574
ACGAACG2550.014.50980415
CTAGCGG3700.014.529
CACCGTT1152.2121758E-514.4782617
GTACGTA908.2797173E-414.38888813
CGAACGA2450.014.3469416
TATACTG4000.014.3374995
GACGGAC5450.014.2568817
GTCTAGC1304.4496555E-614.2307691
CGACGGT3400.014.1470597
TTCGGGC3300.014.01515135
CCGAACA1203.303986E-513.8750019
ACGGACC5500.013.790918
Produced by FastQC (version 0.11.5)