##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3213679
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.867119584750064	33.0	31.0	34.0	30.0	34.0
2	32.04985314339111	33.0	31.0	34.0	30.0	34.0
3	32.207418351366144	34.0	31.0	34.0	30.0	34.0
4	35.84702516959535	37.0	35.0	37.0	35.0	37.0
5	35.763982028074366	37.0	35.0	37.0	35.0	37.0
6	35.82094851414843	37.0	35.0	37.0	35.0	37.0
7	35.78992519165729	37.0	35.0	37.0	35.0	37.0
8	35.77410376082988	37.0	35.0	37.0	35.0	37.0
9	37.48995092540356	39.0	37.0	39.0	35.0	39.0
10	37.40401577133248	39.0	37.0	39.0	34.0	39.0
11	37.48347174686707	39.0	37.0	39.0	35.0	39.0
12	37.343595611136024	39.0	37.0	39.0	34.0	39.0
13	37.397798908976284	39.0	37.0	39.0	34.0	39.0
14	38.63583855139235	40.0	38.0	41.0	34.0	41.0
15	38.62714695524973	40.0	38.0	41.0	34.0	41.0
16	38.62208173249413	40.0	38.0	41.0	34.0	41.0
17	38.574339876509136	40.0	38.0	41.0	34.0	41.0
18	38.54906759511451	40.0	38.0	41.0	34.0	41.0
19	38.564809988800995	40.0	38.0	41.0	34.0	41.0
20	38.51664214129663	40.0	38.0	41.0	34.0	41.0
21	38.475007927051834	40.0	38.0	41.0	34.0	41.0
22	38.45082722947749	40.0	38.0	41.0	34.0	41.0
23	38.40663924430536	40.0	38.0	41.0	34.0	41.0
24	38.39916774512949	40.0	38.0	41.0	34.0	41.0
25	38.37134542684568	40.0	38.0	41.0	34.0	41.0
26	38.21118755171254	40.0	38.0	41.0	34.0	41.0
27	38.11327111388536	40.0	38.0	41.0	33.0	41.0
28	38.041089667014035	40.0	37.0	41.0	33.0	41.0
29	37.97730544961087	40.0	37.0	41.0	33.0	41.0
30	37.909564396444075	40.0	37.0	41.0	33.0	41.0
31	37.77691486921998	40.0	37.0	41.0	33.0	41.0
32	37.68914848060432	40.0	37.0	41.0	33.0	41.0
33	37.630956607676126	40.0	37.0	41.0	32.0	41.0
34	37.50903497206784	40.0	37.0	41.0	32.0	41.0
35	37.44185215760504	40.0	36.0	41.0	32.0	41.0
36	37.32566195939296	40.0	36.0	41.0	31.0	41.0
37	37.2440389970498	39.0	36.0	41.0	31.0	41.0
38	37.16840387605607	39.0	36.0	41.0	31.0	41.0
39	37.032965644670796	39.0	36.0	41.0	31.0	41.0
40	36.93103324880923	39.0	35.0	41.0	31.0	41.0
41	36.78617248331274	39.0	35.0	41.0	30.0	41.0
42	36.72798465559255	39.0	35.0	41.0	30.0	41.0
43	35.57988554550719	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	5.0
12	7.0
13	6.0
14	7.0
15	8.0
16	32.0
17	45.0
18	93.0
19	231.0
20	453.0
21	856.0
22	1581.0
23	2924.0
24	5033.0
25	7960.0
26	12330.0
27	18368.0
28	26141.0
29	36517.0
30	50130.0
31	65608.0
32	84171.0
33	110087.0
34	145014.0
35	195479.0
36	276530.0
37	422443.0
38	733602.0
39	1018009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.302806845363214	18.8956022054474	11.560177603301387	25.241413345888
2	18.61511370612933	20.727334621783942	34.58425685950588	26.073294812580844
3	20.122264855948586	22.183142746988732	29.066406445696664	28.62818595136602
4	13.634622499633597	15.656541925935977	34.61885894639757	36.08997662803286
5	15.007005989086029	36.10273459172493	33.78787987225855	15.10237954693048
6	35.55464002471933	34.564186404429314	15.808828448640948	14.0723451222104
7	30.994881567200704	29.912757310235406	20.390617731266875	18.701743391297015
8	27.9694705040547	33.39477900561942	19.62342847558826	19.012322014737627
9	26.781299563522055	13.521138856743315	19.413233244515087	40.28432833521954
10	17.097476132494876	26.44221778217426	32.97600662667304	23.484299458657816
11	36.61121723731586	21.894439363732346	20.549563288679423	20.944780110272372
12	21.690747582443674	25.00333729659994	29.043442111050915	24.26247300990547
13	30.76688742092785	19.264369590117745	25.021820785461145	24.946922203493255
14	23.056783207034677	20.433216883204576	24.90012225863255	31.609877651128194
15	24.837110364787524	27.71225750922852	22.61028559479649	24.840346531187464
16	26.128931981072164	25.688564414803096	23.72386912320739	24.458634480917354
17	24.03030918769423	26.069405189503993	24.918170109709152	24.982115513092626
18	23.65286016431635	24.597945221037946	26.91594275595042	24.83325185869528
19	25.84638976076951	25.093358733090643	26.078304647103835	22.981946859036015
20	25.58923899991256	24.865209001894716	25.80715124317021	23.738400755022514
21	24.677200180851916	25.172364757027694	25.645747443973093	24.5046876181473
22	25.686946331603128	24.126149500307903	25.954614633259887	24.232289534829086
23	23.328621184629828	24.942410240724104	26.404970751590312	25.323997823055755
24	24.186920971260665	25.69500563061837	25.71607182920261	24.402001568918365
25	24.539756459808213	25.20058786207334	25.977796786797935	24.28185889132051
26	25.16912859062775	25.466482495607064	25.10350287007508	24.260886043690114
27	25.096937186321348	24.861941718510156	24.88717759303278	25.15394350213571
28	24.371600274949675	25.36217836317815	26.119565768703097	24.146655593169076
29	24.72639613352796	25.659407800219004	26.252746462854564	23.361449603398473
30	24.688246710390178	25.168630718873914	25.268765175364432	24.874357395371476
31	23.890189405973654	24.805619976357317	26.234418558916435	25.069772058752598
32	23.464477939458174	25.454191286684203	25.801456834985697	25.279873938871926
33	24.041698004063257	24.56950429709999	26.305832038607463	25.082965660229288
34	25.09102495924453	24.743168188235355	26.320394787407203	23.84541206511291
35	25.269076345210586	24.884906053155902	25.44102880219213	24.404988799441387
36	23.824905972251738	25.202143711304085	25.143145908474366	25.82980440796981
37	24.009709743879213	24.638615119929526	27.051457223948006	24.30021791224326
38	23.74711351071467	24.263064232613154	26.183262236209654	25.806560020462527
39	23.360422742906184	24.985413913461798	27.216937348129665	24.437225995502352
40	24.563685420977016	24.15564840172276	26.645722861555242	24.634943315744977
41	22.816715670731273	24.83844839512596	27.619964532860937	24.724871401281835
42	23.149729640079176	25.37736345167019	26.946530751826803	24.526376156423837
43	23.616048771517008	23.792015319513865	26.89188932684316	25.700046582125967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	646.0
1	617.0
2	588.0
3	1327.5
4	2067.0
5	2067.0
6	2825.0
7	3583.0
8	3284.5
9	2986.0
10	4086.0
11	5186.0
12	5186.0
13	8842.0
14	12498.0
15	17183.5
16	21869.0
17	21333.0
18	20797.0
19	20797.0
20	25061.5
21	29326.0
22	28761.0
23	28196.0
24	32785.5
25	37375.0
26	37375.0
27	42708.0
28	48041.0
29	56070.5
30	64100.0
31	74028.0
32	83956.0
33	83956.0
34	106506.5
35	129057.0
36	139320.5
37	149584.0
38	152820.5
39	156057.0
40	156057.0
41	163178.5
42	170300.0
43	180648.5
44	190997.0
45	193349.0
46	195701.0
47	195701.0
48	197784.0
49	199867.0
50	206823.5
51	213780.0
52	230229.0
53	246678.0
54	246678.0
55	242122.0
56	237566.0
57	216519.0
58	195472.0
59	192744.5
60	190017.0
61	190017.0
62	177314.5
63	164612.0
64	134618.5
65	104625.0
66	94294.5
67	83964.0
68	83964.0
69	76803.0
70	69642.0
71	70684.0
72	71726.0
73	64100.0
74	56474.0
75	56474.0
76	33942.0
77	11410.0
78	8614.0
79	5818.0
80	4672.0
81	3526.0
82	3526.0
83	2775.0
84	2024.0
85	1748.0
86	1472.0
87	1275.5
88	1079.0
89	1079.0
90	961.5
91	844.0
92	473.0
93	102.0
94	76.0
95	50.0
96	50.0
97	33.0
98	16.0
99	10.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3213679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.494641810841216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97320678349854	37.183256038631015
2	11.818079864349967	10.989547803697333
3	3.512075895995527	4.8987813239040365
4	1.4903525332809924	2.771736288271182
5	0.755407757628233	1.7561206556027724
6	0.4827636389546337	1.3467553483496377
7	0.309151785234034	1.0061731063746966
8	0.23181115317919407	0.8622381227859738
9	0.1752481201027145	0.7333288714979093
>10	1.0125587763174748	8.923472054017553
>50	0.1148414076735434	3.7326258942536343
>100	0.09977167422445693	9.331337358534848
>500	0.01182768283344549	3.741357102677742
>1k	0.012365304780341192	10.750105666670214
>5k	5.376219469713561E-4	1.9731643647314616
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9997	0.31107649519444847	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9361	0.29128609297941704	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	7915	0.24629093322637388	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	7867	0.24479731796486207	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7472	0.2325061090420045	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	6896	0.21458272590386282	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	6887	0.21430267304232936	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	6755	0.2101952310731719	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	4964	0.15446471162801262	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	4775	0.14858360153580988	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4426	0.13772377390523446	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	4332	0.13479877735144052	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	4291	0.1335229809822325	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	4280	0.13318069415146938	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	4261	0.13258947144378763	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	4222	0.13137590904380927	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	4190	0.13038016553613474	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	4094	0.12739293501311114	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	4017	0.12499692719776928	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	3974	0.12365889685933162	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	3926	0.12216528159781981	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	3874	0.12054719839784869	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	3872	0.12048496442861904	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	3727	0.11597300165946879	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	3706	0.11531954498255738	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	3546	0.11034082744418468	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	3530	0.10984295569034742	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	3308	0.10293498510585532	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.11169846148293E-5	0.0
6	0.0	0.0	0.0	3.11169846148293E-5	0.0
7	0.0	0.0	0.0	3.11169846148293E-5	0.0
8	0.0	0.0	0.0	3.11169846148293E-5	0.0
9	0.0	0.0	0.0	1.244679384593172E-4	0.0
10	0.0	0.0	0.0	1.244679384593172E-4	0.0
11	0.0	0.0	0.0	1.867019076889758E-4	0.0
12	0.0	0.0	0.0	2.800528615334637E-4	0.0
13	0.0	0.0	0.0	4.6675476922243943E-4	0.0
14	0.0	0.0	0.0	4.6675476922243943E-4	0.0
15	0.0	0.0	0.0	7.468076307559032E-4	0.0
16	0.0	0.0	0.0	0.0012135623999783426	0.0
17	0.0	0.0	0.0	0.0016492001845859528	0.0
18	0.0	0.0	0.0	0.0020537209845787334	0.0
19	0.0	0.0	0.0	0.0024582417845715144	0.0
20	0.0	0.0	0.0	0.003142815446097759	0.0
21	0.0	0.0	0.0	0.00398297403069815	0.0
22	0.0	0.0	0.0	0.005258770399906151	0.0
23	0.0	0.0	0.0	0.0074058423383293725	0.0
24	0.0	0.0	0.0	0.01107764652287923	0.0
25	0.0	0.0	0.0	0.013038016553613476	0.0
26	0.0	0.0	0.0	0.016678703753548504	0.0
27	0.0	0.0	0.0	0.029530018399473	0.0
28	0.0	0.0	0.0	0.06687039993726816	0.0
29	0.0	0.0	0.0	0.11606635261331327	0.0
30	0.0	0.0	0.0	0.17581096307378552	0.0
31	0.0	0.0	0.0	0.357098515439781	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3855	0.0	19.531778	1
CGTTTCG	70	1.2200621E-4	18.5	23
AAGACGG	490	0.0	17.7449	5
CGATAGA	205	0.0	17.146341	37
TCTAGCG	385	0.0	14.896105	28
TAATACG	100	1.094335E-4	14.799999	4
TACGACG	355	0.0	14.591549	5
TTAGACT	330	0.0	14.575757	4
ACGAACG	255	0.0	14.509804	15
CTAGCGG	370	0.0	14.5	29
CACCGTT	115	2.2121758E-5	14.478261	7
GTACGTA	90	8.2797173E-4	14.388888	13
CGAACGA	245	0.0	14.34694	16
TATACTG	400	0.0	14.337499	5
GACGGAC	545	0.0	14.256881	7
GTCTAGC	130	4.4496555E-6	14.230769	1
CGACGGT	340	0.0	14.147059	7
TTCGGGC	330	0.0	14.015151	35
CCGAACA	120	3.303986E-5	13.875001	9
ACGGACC	550	0.0	13.79091	8
>>END_MODULE