FastQCFastQC Report
Fri 10 Feb 2017
ERR1630118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392157
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84822.162909242981765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63361.6156794345121979No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53011.3517545268859157No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31110.7933047223433471No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11890.30319489388178716No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9960.2539799111070311No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.2294999196750281No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA8960.228479920032028No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.22184992235252718No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.19124993306252341No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.17926493725727194No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA4600.11729995894501437No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.11525995965901413No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.11194496081926371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC200.001840457937.022
TTAGACC200.001840457937.04
ATAGACC200.001840457937.03
AGACCCG200.001840457937.05
TCTCAAC200.001840457937.023
ACCCGGT200.001840457937.014
CCCGGTG200.001840457937.015
TGCGGCA303.5951933E-430.83333235
GTGTTTC250.005491983629.59999837
ATTAGAC250.005491983629.5999983
CATTGAT250.005491983629.59999831
ACATTGA250.005491983629.59999830
CGACATT250.005491983629.59999828
CCGACGA250.005491983629.59999824
TATAGAC250.005491983629.5999982
TATACTG250.005491983629.5999985
ACGACAT250.005491983629.59999827
AAATGTA250.005491983629.59999810
CCACGTG754.6020432E-1027.1333336
TCACATT358.858936E-426.4285726
Produced by FastQC (version 0.11.5)