##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630118.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392157
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6207462827388	31.0	31.0	34.0	30.0	34.0
2	31.793462822288014	31.0	31.0	34.0	30.0	34.0
3	31.847581453346493	33.0	31.0	34.0	30.0	34.0
4	35.562736353042276	37.0	35.0	37.0	33.0	37.0
5	35.53639231226269	37.0	35.0	37.0	33.0	37.0
6	35.63507472772385	37.0	35.0	37.0	33.0	37.0
7	35.60539528811165	37.0	35.0	37.0	33.0	37.0
8	35.65033137238402	37.0	35.0	37.0	33.0	37.0
9	37.306499692725104	39.0	37.0	39.0	34.0	39.0
10	37.19868063046178	39.0	37.0	39.0	34.0	39.0
11	37.29311729740895	39.0	37.0	39.0	34.0	39.0
12	37.22488697128956	39.0	37.0	39.0	34.0	39.0
13	37.27276830453109	39.0	37.0	39.0	34.0	39.0
14	38.32565528602065	40.0	38.0	41.0	34.0	41.0
15	38.30913639180226	40.0	38.0	41.0	34.0	41.0
16	38.21286882549591	40.0	38.0	41.0	33.0	41.0
17	38.317181638986426	40.0	38.0	41.0	34.0	41.0
18	38.34278873002395	40.0	38.0	41.0	34.0	41.0
19	38.40896375686268	40.0	38.0	41.0	34.0	41.0
20	38.41091195618082	40.0	38.0	41.0	34.0	41.0
21	38.36281387301514	40.0	38.0	41.0	34.0	41.0
22	38.31929813824565	40.0	38.0	41.0	34.0	41.0
23	38.24860196298931	40.0	38.0	41.0	34.0	41.0
24	38.180588386794064	40.0	37.0	41.0	33.0	41.0
25	38.152801046519635	40.0	37.0	41.0	33.0	41.0
26	37.894231137019105	40.0	37.0	41.0	33.0	41.0
27	37.70840505205823	40.0	37.0	41.0	33.0	41.0
28	37.594792391822665	40.0	36.0	41.0	33.0	41.0
29	37.459224239271514	39.0	36.0	41.0	32.0	41.0
30	37.250221212422574	39.0	36.0	41.0	32.0	41.0
31	37.045193634182226	39.0	35.0	41.0	32.0	41.0
32	36.835703557503756	39.0	35.0	41.0	31.0	41.0
33	36.669757265584956	39.0	35.0	40.0	31.0	41.0
34	36.56162710343052	39.0	35.0	40.0	31.0	41.0
35	36.32563233602868	38.0	35.0	40.0	30.0	41.0
36	36.12001825799361	38.0	35.0	40.0	30.0	41.0
37	35.92321952687317	38.0	35.0	40.0	30.0	41.0
38	35.719079348322225	38.0	35.0	40.0	28.0	41.0
39	35.509885071540225	38.0	35.0	40.0	27.0	41.0
40	35.22665157067195	38.0	35.0	40.0	25.0	41.0
41	34.93354447325943	38.0	34.0	40.0	24.0	41.0
42	34.690009358496724	38.0	34.0	40.0	22.0	41.0
43	33.52126061755878	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	4.0
12	2.0
13	1.0
14	1.0
15	2.0
16	3.0
17	6.0
18	16.0
19	31.0
20	107.0
21	188.0
22	362.0
23	640.0
24	1079.0
25	1617.0
26	2464.0
27	3571.0
28	4936.0
29	6435.0
30	8422.0
31	10379.0
32	13029.0
33	16488.0
34	21557.0
35	27411.0
36	38214.0
37	60928.0
38	85680.0
39	88577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0609245786764	20.831452708991552	14.179780037076986	20.927842675255064
2	18.436238547316506	24.498861425398502	34.2765780031977	22.78832202408729
3	20.131987953804217	27.49638537626512	30.497224325971484	21.87440234395918
4	13.80340016880994	17.511353871026145	35.58115754659486	33.10408841356905
5	12.218320723587746	40.73470574285299	33.35398832610409	13.692985207455177
6	29.408119707158104	37.23432196798731	17.164298992495354	16.193259332359233
7	25.838630956479165	32.936808472117036	23.238396866561096	17.986163704842703
8	28.48961002863649	32.83123850906652	20.137852951751466	18.54129851054552
9	27.034070538075312	13.333945333119132	22.36298217295624	37.26900195584932
10	19.01100834614708	28.942489870128547	30.719074248324013	21.32742753540036
11	31.312714040550087	25.7256659960169	20.694262757008037	22.26735720642498
12	19.76121808357367	31.4422539952111	28.636489977228507	20.16003794398672
13	30.37660936818672	20.993887652139325	26.405495758076487	22.22400722159747
14	22.420357152875	23.10605191288183	29.204884778290328	25.26870615595285
15	25.45052109231762	28.30805009218247	25.285791149973097	20.955637665526815
16	20.8046777183628	27.222515472119586	28.390160063443975	23.58264674607364
17	19.656923120076907	29.414494704926852	28.870834895207786	22.057747279788455
18	18.986018354893574	26.45700574004799	31.399414010205096	23.157561894853337
19	20.118472958534465	27.48491038028137	33.53758826184411	18.859028399340062
20	20.686102759864035	26.827775610278536	33.500103274963855	18.986018354893574
21	21.522502467124134	26.819105613313038	31.783698875705397	19.874693043857437
22	21.811417366003923	26.69747065588527	31.65339392131213	19.837718056798682
23	20.735827742460287	27.640970325660387	31.804863868297645	19.818338063581677
24	19.308083242170866	29.114359809974065	31.10488911328881	20.47266783456626
25	20.411467855986253	28.1762151383247	32.01141379600517	19.400903209683875
26	19.91268803055919	28.73721494197477	31.645233924168124	19.704863103297914
27	19.209908276532104	29.226304770793334	31.423129001904847	20.140657950769718
28	19.33077823422762	29.023324841836306	32.93604347238478	18.709853451551293
29	20.07410297406396	28.78668492466028	32.2490737128242	18.890138388451565
30	19.376933218073376	30.108604461988435	31.670478915332378	18.843983404605808
31	20.0004079998572	29.208454777040828	31.770948880167893	19.02018834293408
32	18.27354860425799	29.517769668780613	31.98565880501942	20.223022921941975
33	18.37682356811175	29.823769561680653	32.14375874968444	19.655648120523157
34	19.136468302236096	28.551065007127246	32.08510877021193	20.227357920424726
35	19.153808296167096	29.679184612285386	31.75896388436264	19.40804320718488
36	19.08827331910434	29.789344573729398	30.70173425439301	20.420647852773254
37	18.905438383096566	28.74511993920802	31.85688385009065	20.49255782760476
38	18.69353345726329	29.497369675920616	31.724283896500637	20.08481297031546
39	17.86274374803969	28.80351491876978	32.96638846176404	20.367352871426494
40	18.20010862996198	28.995784851475303	33.36393332262333	19.44017319593938
41	17.893088737418942	28.696924956076263	32.83353350826327	20.576452798241522
42	17.347133928503126	29.23726976695558	32.852148501748026	20.56344780279327
43	17.588108844161905	28.652809971516508	32.72796354521276	21.031117639108825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	337.0
1	244.5
2	152.0
3	534.0
4	916.0
5	916.0
6	1319.0
7	1722.0
8	1699.5
9	1677.0
10	2329.0
11	2981.0
12	2981.0
13	5727.5
14	8474.0
15	13053.0
16	17632.0
17	14643.5
18	11655.0
19	11655.0
20	12011.0
21	12367.0
22	8560.0
23	4753.0
24	4944.5
25	5136.0
26	5136.0
27	5585.5
28	6035.0
29	7162.5
30	8290.0
31	9394.5
32	10499.0
33	10499.0
34	12978.0
35	15457.0
36	17013.5
37	18570.0
38	20044.0
39	21518.0
40	21518.0
41	24458.5
42	27399.0
43	29009.0
44	30619.0
45	32155.0
46	33691.0
47	33691.0
48	34158.5
49	34626.0
50	31312.5
51	27999.0
52	25793.0
53	23587.0
54	23587.0
55	21761.5
56	19936.0
57	17311.0
58	14686.0
59	13000.5
60	11315.0
61	11315.0
62	9434.5
63	7554.0
64	5903.5
65	4253.0
66	3389.0
67	2525.0
68	2525.0
69	2006.0
70	1487.0
71	1169.5
72	852.0
73	899.0
74	946.0
75	946.0
76	811.0
77	676.0
78	656.5
79	637.0
80	530.0
81	423.0
82	423.0
83	373.0
84	323.0
85	277.5
86	232.0
87	192.5
88	153.0
89	153.0
90	103.0
91	53.0
92	32.5
93	12.0
94	6.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	392157.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.11441736244627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11600696585684	23.15963546214609
2	11.051000916198706	7.097929113911627
3	4.486797842007924	4.322726955574971
4	2.481603208877477	3.187809647115089
5	1.701746282096338	2.7325295174116517
6	1.157411353657365	2.230175476283191
7	0.8874884870743276	1.9950822974789668
8	0.6719253229479731	1.7262792206038162
9	0.5971658311187317	1.7259869461625148
>10	4.253283021841569	27.100005138150017
>50	0.43256408273373065	9.51749480469379
>100	0.15425998538666133	7.662565174115173
>500	0.004770927383092618	1.3061625909154233
>1k	0.0015903091276975392	1.0980447978370127
>5k	0.002385463691546309	5.137572857600665
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8482	2.162909242981765	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6336	1.6156794345121979	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5301	1.3517545268859157	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3111	0.7933047223433471	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1189	0.30319489388178716	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	996	0.2539799111070311	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.2294999196750281	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	896	0.228479920032028	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	870	0.22184992235252718	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	750	0.19124993306252341	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	703	0.17926493725727194	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	460	0.11729995894501437	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	452	0.11525995965901413	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	439	0.11194496081926371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.549999107500312E-4	0.0
8	0.0	0.0	0.0	2.549999107500312E-4	0.0
9	0.0	0.0	0.0	2.549999107500312E-4	0.0
10	0.0	0.0	0.0	2.549999107500312E-4	0.0
11	0.0	0.0	0.0	5.099998215000624E-4	0.0
12	0.0	0.0	0.0	5.099998215000624E-4	0.0
13	0.0	0.0	0.0	5.099998215000624E-4	0.0
14	0.0	0.0	0.0	5.099998215000624E-4	0.0
15	0.0	0.0	0.0	5.099998215000624E-4	0.0
16	0.0	0.0	0.0	0.003314998839750406	0.0
17	0.0	0.0	0.0	0.005099998215000625	0.0
18	0.0	0.0	0.0	0.005864997947250718	0.0
19	0.0	0.0	0.0	0.009179996787001125	0.0
20	0.0	2.549999107500312E-4	0.0	0.010709996251501312	0.0
21	0.0	2.549999107500312E-4	0.0	0.013259995359001624	0.0
22	0.0	2.549999107500312E-4	0.0	0.017594993841752154	0.0
23	0.0	2.549999107500312E-4	0.0	0.026264990807253218	0.0
24	0.0	2.549999107500312E-4	0.0	0.04283998500600525	0.0
25	0.0	2.549999107500312E-4	0.0	0.04844998304250594	0.0
26	0.0	2.549999107500312E-4	0.0	0.05609998036500687	0.0
27	0.0	2.549999107500312E-4	0.0	0.07496997376050918	0.0
28	0.0	2.549999107500312E-4	0.0	0.12086995769551481	0.0
29	0.0	2.549999107500312E-4	0.0	0.19634993127752406	0.0
30	0.0	2.549999107500312E-4	0.0	0.3422098802265419	0.0
31	0.0	2.549999107500312E-4	0.0	0.8124297156495995	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTAGC	20	0.0018404579	37.0	22
TTAGACC	20	0.0018404579	37.0	4
ATAGACC	20	0.0018404579	37.0	3
AGACCCG	20	0.0018404579	37.0	5
TCTCAAC	20	0.0018404579	37.0	23
ACCCGGT	20	0.0018404579	37.0	14
CCCGGTG	20	0.0018404579	37.0	15
TGCGGCA	30	3.5951933E-4	30.833332	35
GTGTTTC	25	0.0054919836	29.599998	37
ATTAGAC	25	0.0054919836	29.599998	3
CATTGAT	25	0.0054919836	29.599998	31
ACATTGA	25	0.0054919836	29.599998	30
CGACATT	25	0.0054919836	29.599998	28
CCGACGA	25	0.0054919836	29.599998	24
TATAGAC	25	0.0054919836	29.599998	2
TATACTG	25	0.0054919836	29.599998	5
ACGACAT	25	0.0054919836	29.599998	27
AAATGTA	25	0.0054919836	29.599998	10
CCACGTG	75	4.6020432E-10	27.133333	6
TCACATT	35	8.858936E-4	26.42857	26
>>END_MODULE