FastQCFastQC Report
Fri 10 Feb 2017
ERR1630117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414674
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71220.2949466470422094No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG63060.2611532654097406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.2608633712045601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53140.22007111518987654No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG47790.19791491522251037No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT42950.17787080160717347No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG37930.1570812457499439No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC37330.15459643827696823No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC36090.14946116949948524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.1471834293159242No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA33700.13956335306546558No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC32900.13625027643483137No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC29320.12142425851274334No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC28200.11678595122985545No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA27620.11438397067264566No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA27410.11351428805710419No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT26900.1114022017050749No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT26220.10858608656903583No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC26050.10788205778502606No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT26030.10779923086926019No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG25600.1060184521802943No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA24740.10245689480236256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.020.7745911
TCTAGCG1900.019.47368428
TGCGACA609.24083E-418.519
GCGCTAT500.0070370718.520
CTAGCGG2150.017.20930329
GGACCGT803.3844946E-416.18756
AGCGTCA2003.6379788E-1215.7253
CGGTCCA2600.015.65384610
CTTATAC10600.015.35849137
TATACTG3100.014.9193555
ACCGTTC1001.0941556E-414.88
CGCAATA2550.014.50980436
TTTCGGA2800.013.87530
GTATAGA2850.013.6315791
TCGTTTA2457.2759576E-1213.59183830
GTATTAG4500.013.5666671
CCGGTCC3300.013.4545459
GTATCAA37950.013.4057972
ATACTGG3750.013.3200016
CTAGGAC3950.013.1139243
Produced by FastQC (version 0.11.5)