##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630117.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2414674
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.855044614718178	33.0	31.0	34.0	30.0	34.0
2	32.0630511613576	33.0	31.0	34.0	30.0	34.0
3	32.194820501649495	34.0	31.0	34.0	30.0	34.0
4	35.82522278369668	37.0	35.0	37.0	35.0	37.0
5	35.75570366848692	37.0	35.0	37.0	35.0	37.0
6	35.80300446354249	37.0	35.0	37.0	35.0	37.0
7	35.77476918209249	37.0	35.0	37.0	35.0	37.0
8	35.77557177490626	37.0	35.0	37.0	35.0	37.0
9	37.53007486724916	39.0	37.0	39.0	35.0	39.0
10	37.42269515470826	39.0	37.0	39.0	34.0	39.0
11	37.49173263140283	39.0	37.0	39.0	35.0	39.0
12	37.388367539469094	39.0	37.0	39.0	34.0	39.0
13	37.414083640276075	39.0	37.0	39.0	34.0	39.0
14	38.66944274879342	40.0	38.0	41.0	34.0	41.0
15	38.671029298364914	40.0	38.0	41.0	34.0	41.0
16	38.63840543278306	40.0	38.0	41.0	34.0	41.0
17	38.588553568721906	40.0	38.0	41.0	34.0	41.0
18	38.54470210057341	40.0	38.0	41.0	34.0	41.0
19	38.57773430284999	40.0	38.0	41.0	34.0	41.0
20	38.55674679066408	40.0	38.0	41.0	34.0	41.0
21	38.50247569651224	40.0	38.0	41.0	34.0	41.0
22	38.46269765608111	40.0	38.0	41.0	34.0	41.0
23	38.416696001199334	40.0	38.0	41.0	34.0	41.0
24	38.37888302934475	40.0	38.0	41.0	34.0	41.0
25	38.357786185630026	40.0	38.0	41.0	34.0	41.0
26	38.19744611487928	40.0	38.0	41.0	33.0	41.0
27	38.07001731910809	40.0	38.0	41.0	33.0	41.0
28	38.01936037742569	40.0	37.0	41.0	33.0	41.0
29	37.955058115505445	40.0	37.0	41.0	33.0	41.0
30	37.883082353974075	40.0	37.0	41.0	33.0	41.0
31	37.80079712623733	40.0	37.0	41.0	33.0	41.0
32	37.71450804539246	40.0	37.0	41.0	33.0	41.0
33	37.63164468578367	40.0	37.0	41.0	32.0	41.0
34	37.53925457432349	40.0	37.0	41.0	32.0	41.0
35	37.437919570095175	40.0	36.0	41.0	32.0	41.0
36	37.34259779995146	40.0	36.0	41.0	31.0	41.0
37	37.26340160203821	40.0	36.0	41.0	31.0	41.0
38	37.18343097246254	39.0	36.0	41.0	31.0	41.0
39	37.064191687987694	39.0	36.0	41.0	31.0	41.0
40	36.96437448699079	39.0	36.0	41.0	31.0	41.0
41	36.83576375113162	39.0	35.0	41.0	30.0	41.0
42	36.79123103160095	39.0	35.0	41.0	30.0	41.0
43	35.587845812726684	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	5.0
12	3.0
13	2.0
14	2.0
15	5.0
16	25.0
17	36.0
18	62.0
19	156.0
20	352.0
21	710.0
22	1292.0
23	2375.0
24	3921.0
25	6172.0
26	9417.0
27	13928.0
28	20242.0
29	27944.0
30	37851.0
31	49458.0
32	63614.0
33	81862.0
34	107828.0
35	144156.0
36	202898.0
37	310152.0
38	548292.0
39	781901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.939388919580864	19.166521029339776	11.725640811140552	25.168449239938806
2	19.05946724071241	21.069883553639123	34.436698287222214	25.433950918426255
3	20.679147578513703	22.4057988780266	28.840207829297036	28.074845714162656
4	14.18638706508622	15.70489432527952	34.55663166125117	35.55208694838309
5	15.29150518869214	35.92120509849362	33.52423556968767	15.263054143126567
6	35.2653401660017	34.547727767806336	15.786685904598302	14.400246161593655
7	30.67913101313055	29.79793545629762	20.572507924465167	18.950425606106663
8	27.5670338936022	33.12066142261854	19.97582282328795	19.33648186049131
9	26.812232210227965	13.657247313716054	19.809547789887993	39.72097268616799
10	17.159790514164644	26.39826328523022	33.41059704125692	23.03134915934822
11	36.082758997694924	21.96627784951509	20.673515348241626	21.27744780454836
12	21.43096749292037	25.100945303589633	29.49118597375878	23.97690122973122
13	30.639995295431184	19.80416404036321	24.948005403627985	24.60783526057762
14	23.251296034164444	20.867785879170437	24.970244430511116	30.910673656153996
15	25.15250505865388	27.732894792423323	22.87406084630886	24.240539302613932
16	26.063766785909813	25.84481383408278	23.650356114324335	24.44106326568307
17	24.022373206486673	25.886392945797237	25.153871702764018	24.937362144952072
18	23.484122494382266	24.61210084673956	27.018429817027062	24.885346841851117
19	25.293932017324078	25.275378788192526	26.339787482699528	23.090901711783868
20	25.510234507846608	24.980390727692434	25.97837223575522	23.531002528705738
21	24.72896962488518	25.236532964698338	25.596208846411567	24.438288564004914
22	25.802737760873722	24.211384228264354	26.045420624067678	23.940457386794243
23	23.633500836965986	25.018946656981438	26.38281606543989	24.964736440612686
24	24.149512522187262	25.856409602289997	25.883576830661198	24.110501044861543
25	24.65011840107609	25.465922107911876	25.84696733389269	24.036992157119347
26	25.127781224297774	25.425088438439307	25.179879354314494	24.267250982948426
27	25.156522164068523	24.85147063330288	25.04992392347787	24.942083279150726
28	24.541739381796464	25.179299565904135	25.997132532176188	24.281828520123213
29	24.49017962673222	25.71258066306259	26.420502312113353	23.376737398091834
30	24.638895353989813	25.37087822207056	25.462526204365478	24.527700219574154
31	24.012185495847472	24.931274366643283	26.310756648723594	24.74578348878565
32	23.546656815785486	25.571236531308163	25.965244169606333	24.916862483300022
33	24.018438927987795	24.72710601928045	26.390436141690348	24.86401891104141
34	25.060484355238017	24.758870141476656	26.608477997443963	23.572167505841367
35	25.04466441432674	24.87271573719682	25.67033893602201	24.412280912454435
36	23.795344630372465	25.33894844604282	25.27939589360717	25.586311029977544
37	23.93730996399514	24.792580696193358	27.086554955244473	24.183554384567028
38	23.74453031755011	24.300505989628412	26.317134321237567	25.63782937158391
39	23.404525828331277	24.912224176017137	27.446893452283827	24.23635654336776
40	24.340097255364494	24.182933182698783	26.895100539451704	24.581869022485023
41	23.115956853803038	24.79916543599674	27.517669051805747	24.567208658394467
42	23.107301441105506	25.52087776652252	27.039799161294653	24.33202163107732
43	23.600245830285992	23.69413013930659	26.99329184809212	25.712332182315293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	659.0
1	612.5
2	566.0
3	1155.0
4	1744.0
5	1744.0
6	2318.5
7	2893.0
8	2616.5
9	2340.0
10	3204.5
11	4069.0
12	4069.0
13	7034.5
14	10000.0
15	13017.5
16	16035.0
17	15925.5
18	15816.0
19	15816.0
20	18999.0
21	22182.0
22	22489.0
23	22796.0
24	26676.0
25	30556.0
26	30556.0
27	34711.0
28	38866.0
29	44761.5
30	50657.0
31	57458.5
32	64260.0
33	64260.0
34	81802.0
35	99344.0
36	106679.0
37	114014.0
38	114539.0
39	115064.0
40	115064.0
41	118927.0
42	122790.0
43	131622.0
44	140454.0
45	140596.0
46	140738.0
47	140738.0
48	142984.0
49	145230.0
50	151800.0
51	158370.0
52	172145.0
53	185920.0
54	185920.0
55	185511.0
56	185102.0
57	170824.0
58	156546.0
59	152415.5
60	148285.0
61	148285.0
62	136645.5
63	125006.0
64	103169.5
65	81333.0
66	72121.0
67	62909.0
68	62909.0
69	55851.0
70	48793.0
71	48272.0
72	47751.0
73	41113.0
74	34475.0
75	34475.0
76	21444.0
77	8413.0
78	6404.0
79	4395.0
80	3552.0
81	2709.0
82	2709.0
83	2102.5
84	1496.0
85	1207.5
86	919.0
87	786.0
88	653.0
89	653.0
90	535.0
91	417.0
92	243.0
93	69.0
94	48.0
95	27.0
96	27.0
97	16.5
98	6.0
99	6.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2414674.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.032657952608844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34303687130003	43.66870109174712
2	11.057434807426974	11.728103159510919
3	2.9037073066597427	4.619739491657317
4	1.1971069669504553	2.5394305724387403
5	0.5792871040234019	1.5360567422015203
6	0.35169058162798594	1.1190651792378623
7	0.2460201016969094	0.9132969931930768
8	0.16712436683697468	0.7090439505609282
9	0.13989544795572484	0.6677124696506653
>10	0.8447561514109546	8.552398758473341
>50	0.08505353577435863	3.1417867897696725
>100	0.06396549137799783	6.936366635407833
>500	0.011988591178539128	4.561999237644634
>1k	0.008619248559733965	8.265727775323734
>5k	3.134272203539623E-4	1.0405711531827222
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7122	0.2949466470422094	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	6306	0.2611532654097406	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6299	0.2608633712045601	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5314	0.22007111518987654	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	4779	0.19791491522251037	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	4295	0.17787080160717347	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	3793	0.1570812457499439	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	3733	0.15459643827696823	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	3609	0.14946116949948524	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3554	0.1471834293159242	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	3370	0.13956335306546558	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	3290	0.13625027643483137	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	2932	0.12142425851274334	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	2820	0.11678595122985545	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	2762	0.11438397067264566	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	2741	0.11351428805710419	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	2690	0.1114022017050749	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	2622	0.10858608656903583	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	2605	0.10788205778502606	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	2603	0.10779923086926019	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	2560	0.1060184521802943	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	2474	0.10245689480236256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.141345788292747E-5	0.0	0.0	0.0	0.0
6	4.141345788292747E-5	0.0	0.0	0.0	0.0
7	4.141345788292747E-5	0.0	0.0	0.0	0.0
8	4.141345788292747E-5	0.0	0.0	0.0	0.0
9	4.141345788292747E-5	0.0	0.0	4.141345788292747E-5	0.0
10	4.141345788292747E-5	0.0	0.0	4.141345788292747E-5	8.282691576585494E-5
11	4.141345788292747E-5	0.0	0.0	2.484807472975648E-4	8.282691576585494E-5
12	4.141345788292747E-5	0.0	0.0	2.484807472975648E-4	8.282691576585494E-5
13	4.141345788292747E-5	0.0	0.0	2.484807472975648E-4	8.282691576585494E-5
14	4.141345788292747E-5	0.0	0.0	2.484807472975648E-4	8.282691576585494E-5
15	4.141345788292747E-5	0.0	0.0	2.484807472975648E-4	8.282691576585494E-5
16	4.141345788292747E-5	0.0	0.0	4.141345788292747E-4	8.282691576585494E-5
17	4.141345788292747E-5	0.0	0.0	7.040287840097669E-4	8.282691576585494E-5
18	4.141345788292747E-5	0.0	0.0	8.696826155414768E-4	8.282691576585494E-5
19	4.141345788292747E-5	0.0	0.0	0.0010353364470731866	8.282691576585494E-5
20	4.141345788292747E-5	0.0	0.0	0.0012009902786048966	8.282691576585494E-5
21	4.141345788292747E-5	0.0	0.0	0.0014494710259024613	1.6565383153170987E-4
22	4.141345788292747E-5	0.0	0.0	0.0019878459783805183	1.6565383153170987E-4
23	4.141345788292747E-5	0.0	0.0	0.0031060093412195603	1.6565383153170987E-4
24	4.141345788292747E-5	0.0	0.0	0.004845374572302514	1.6565383153170987E-4
25	4.141345788292747E-5	0.0	0.0	0.00637767251397083	1.6565383153170987E-4
26	4.141345788292747E-5	0.0	0.0	0.009069547276361115	1.6565383153170987E-4
27	4.141345788292747E-5	0.0	0.0	0.01991987324168811	2.0706728941463734E-4
28	4.141345788292747E-5	0.0	0.0	0.054210216368752055	2.0706728941463734E-4
29	4.141345788292747E-5	0.0	0.0	0.09802565480888932	2.0706728941463734E-4
30	4.141345788292747E-5	0.0	0.0	0.15053791940444133	2.0706728941463734E-4
31	4.141345788292747E-5	0.0	0.0	0.32223811578705863	2.0706728941463734E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2440	0.0	20.774591	1
TCTAGCG	190	0.0	19.473684	28
TGCGACA	60	9.24083E-4	18.5	19
GCGCTAT	50	0.00703707	18.5	20
CTAGCGG	215	0.0	17.209303	29
GGACCGT	80	3.3844946E-4	16.1875	6
AGCGTCA	200	3.6379788E-12	15.725	3
CGGTCCA	260	0.0	15.653846	10
CTTATAC	1060	0.0	15.358491	37
TATACTG	310	0.0	14.919355	5
ACCGTTC	100	1.0941556E-4	14.8	8
CGCAATA	255	0.0	14.509804	36
TTTCGGA	280	0.0	13.875	30
GTATAGA	285	0.0	13.631579	1
TCGTTTA	245	7.2759576E-12	13.591838	30
GTATTAG	450	0.0	13.566667	1
CCGGTCC	330	0.0	13.454545	9
GTATCAA	3795	0.0	13.405797	2
ATACTGG	375	0.0	13.320001	6
CTAGGAC	395	0.0	13.113924	3
>>END_MODULE