FastQCFastQC Report
Fri 10 Feb 2017
ERR1630116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255075
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46730.37232834691153915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38650.30794972412007254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30750.24500527856900983No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.14517060733422307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGAT851.2454948E-619.58823614
GGCTACG951.676417E-719.4736841
CGCGTAA1052.2599124E-819.3809510
CGCGCTT701.2193107E-418.512
GTTCGCG701.2193107E-418.530
CTAGTAC609.23746E-418.53
CGAGCGT1002.8754403E-718.4999986
AGTCGGT1156.406299E-817.69565211
GCTACGA1054.79813E-717.6190472
CGAATTA1401.869921E-917.17857215
GTTCTAA1301.3933459E-817.0769231
GCCTATG650.001580043717.0769237
GGTATCA17650.016.8753551
TCTTATA9950.016.73366737
TAAGACT1251.6579543E-716.284
TCGCGTA1251.6579543E-716.289
TCGCCAT2650.016.05660413
TCGTTAA700.00259267215.85714228
CTCGTTA700.00259267215.85714227
TAGCGTA700.00259267215.8571427
Produced by FastQC (version 0.11.5)