##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630116.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1255075
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99841682767962	31.0	31.0	34.0	28.0	34.0
2	31.245422783498995	31.0	31.0	34.0	28.0	34.0
3	31.35631416449216	31.0	31.0	34.0	28.0	34.0
4	35.177321674003544	37.0	35.0	37.0	32.0	37.0
5	34.83660418700077	35.0	35.0	37.0	32.0	37.0
6	34.9138625181762	35.0	35.0	37.0	32.0	37.0
7	34.926787642172776	35.0	35.0	37.0	32.0	37.0
8	34.92127880803936	35.0	35.0	37.0	32.0	37.0
9	36.55295978327988	38.0	35.0	39.0	32.0	39.0
10	36.28312331932354	38.0	35.0	39.0	32.0	39.0
11	36.43399557795351	38.0	35.0	39.0	32.0	39.0
12	36.321420632233135	38.0	35.0	39.0	32.0	39.0
13	36.37897097783001	38.0	35.0	39.0	32.0	39.0
14	37.374403920084454	39.0	36.0	40.0	32.0	41.0
15	37.34069278728363	39.0	36.0	40.0	32.0	41.0
16	37.267614285998846	39.0	36.0	40.0	32.0	41.0
17	37.220092026372924	39.0	36.0	40.0	32.0	41.0
18	37.15877298169432	39.0	36.0	40.0	32.0	41.0
19	37.19133358564229	39.0	36.0	40.0	32.0	41.0
20	37.15556361173635	39.0	36.0	40.0	32.0	41.0
21	37.0868497898532	39.0	36.0	40.0	31.0	41.0
22	37.04009401828576	39.0	36.0	40.0	31.0	41.0
23	36.973991992510406	39.0	36.0	40.0	31.0	41.0
24	36.92002549648427	39.0	36.0	40.0	31.0	41.0
25	36.90834332609605	39.0	36.0	40.0	31.0	41.0
26	36.67429595840886	38.0	36.0	40.0	30.0	41.0
27	36.544061510268314	38.0	35.0	40.0	30.0	41.0
28	36.45687229846822	38.0	35.0	40.0	30.0	41.0
29	36.3767958090154	38.0	35.0	40.0	30.0	41.0
30	36.293784833575685	38.0	35.0	40.0	30.0	41.0
31	36.217030057964664	38.0	35.0	40.0	30.0	41.0
32	36.10352210027289	38.0	35.0	40.0	30.0	41.0
33	35.99771169053642	38.0	35.0	40.0	29.0	41.0
34	35.92103499790849	38.0	35.0	40.0	29.0	41.0
35	35.76767922235723	38.0	34.0	40.0	29.0	41.0
36	35.66080353763719	38.0	34.0	40.0	28.0	41.0
37	35.59357807302352	38.0	34.0	40.0	27.0	41.0
38	35.46678405672968	38.0	34.0	40.0	27.0	41.0
39	35.36446507180846	38.0	34.0	40.0	27.0	41.0
40	35.24421648108679	38.0	34.0	40.0	27.0	41.0
41	35.12908471605282	38.0	34.0	40.0	26.0	41.0
42	35.077186622313405	38.0	33.0	40.0	26.0	41.0
43	33.80364599725116	37.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	4.0
12	3.0
13	4.0
14	13.0
15	13.0
16	36.0
17	76.0
18	134.0
19	261.0
20	515.0
21	931.0
22	1717.0
23	2877.0
24	4589.0
25	6693.0
26	9799.0
27	13941.0
28	19153.0
29	25273.0
30	32832.0
31	42100.0
32	53425.0
33	68801.0
34	89572.0
35	120392.0
36	168326.0
37	233806.0
38	262756.0
39	97030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.51423221719818	20.664183415333746	13.637830408541321	25.183753958926758
2	18.40455749656395	22.310061151724	34.87687986773699	24.408501483975062
3	19.98621596318945	23.866940222695852	30.48869589466765	25.658147919447043
4	15.606477700535827	16.70601358484553	34.33579666553791	33.35171204908073
5	14.475708622990657	37.012449455211836	34.21118259864948	14.300659323148018
6	33.72770551560664	35.912037129255225	15.820887197976216	14.539370157161924
7	28.343565125590104	31.486723900962094	21.640698763022133	18.529012210425673
8	26.5783319721929	34.96755173993586	20.11991315260044	18.3342031352708
9	26.49626516343645	14.893611935541701	20.141027428639724	38.46909547238213
10	16.65462223373105	27.79913550983009	33.31992112025178	22.22632113618708
11	34.82963169531701	22.469812561002332	21.954863255183955	20.745692488496704
12	20.75238531561859	26.17094595940482	29.97215305858216	23.104515666394438
13	29.625161842917752	20.323167938171025	25.540704738760635	24.51096548015059
14	22.264326833057783	21.382945242316197	25.874469653207978	30.478258271418042
15	24.671593331075833	28.42451646315957	22.847319881281994	24.0565703244826
16	24.00039838256678	27.47190406947792	24.637093400792782	23.89060414716252
17	22.864848714220265	27.409597036033702	26.22990657928809	23.49564767045794
18	22.790829233312753	26.19524729597833	27.426329103838416	23.58759436687051
19	23.65866581678386	26.32113618708045	27.61524211700496	22.40495587913073
20	24.08334163297014	25.844112901619425	27.175348086767727	22.897197378642712
21	23.812839870127284	26.307750532836682	26.46551002928112	23.413899567754914
22	23.89060414716252	26.06123140051391	26.71043563133677	23.337728820986793
23	23.33366531880565	26.24536382287911	26.82708204688963	23.59388881142561
24	23.832201262872736	26.262733302790668	26.735294703503776	23.16977073083282
25	23.972192896838834	25.750253968886323	26.888432962173574	23.38912017210127
26	23.64695336932056	26.34527817062725	26.718881341752486	23.2888871182997
27	23.653964902495865	26.232854610282253	26.460251379399637	23.65292910782224
28	23.287134235005876	26.38910025297293	27.009700615501064	23.31406489652013
29	23.212796048044936	26.53530665498078	27.091209688664026	23.16068760831026
30	23.15654442961576	27.142521363265143	26.667569667151366	23.03336453996773
31	23.503535645280163	26.548293926657767	26.75648865605641	23.19168177200566
32	23.024201740931815	26.417385415214227	27.07519470947951	23.48321813437444
33	23.056152022787483	26.330298986116368	27.081090771467842	23.532458219628307
34	23.536920104376232	26.050395394697528	26.91130012150668	23.50138437941956
35	23.4115889488676	26.096926478497302	27.266657371073443	23.22482720156166
36	23.147301954066492	26.151903272712783	27.032408421807464	23.66838635141326
37	23.564966237077464	25.217536800589606	27.33932235125391	23.87817461107902
38	23.01456088281577	25.657590183853557	27.821843316136487	23.50600561719419
39	22.900942174770435	25.48102703025716	28.133059777304144	23.484971017668265
40	22.56956755572376	25.28279186502799	28.553831444335998	23.593809134912256
41	22.221859251439156	25.399916339660976	28.726888831344738	23.651335577555127
42	22.5314821823397	25.221600302770753	28.445471386172137	23.80144612871741
43	22.176602991853077	25.121048542915762	28.62075971555485	24.081588749676314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	263.0
1	235.0
2	207.0
3	418.5
4	630.0
5	630.0
6	884.5
7	1139.0
8	1155.5
9	1172.0
10	1644.0
11	2116.0
12	2116.0
13	3824.5
14	5533.0
15	8566.5
16	11600.0
17	11022.0
18	10444.0
19	10444.0
20	12764.0
21	15084.0
22	15601.0
23	16118.0
24	18724.0
25	21330.0
26	21330.0
27	23758.5
28	26187.0
29	30651.5
30	35116.0
31	38543.5
32	41971.0
33	41971.0
34	46354.5
35	50738.0
36	54934.5
37	59131.0
38	64064.5
39	68998.0
40	68998.0
41	73885.5
42	78773.0
43	77602.5
44	76432.0
45	79729.0
46	83026.0
47	83026.0
48	84474.5
49	85923.0
50	87986.5
51	90050.0
52	92681.0
53	95312.0
54	95312.0
55	87575.5
56	79839.0
57	75365.0
58	70891.0
59	64419.5
60	57948.0
61	57948.0
62	53504.5
63	49061.0
64	43671.0
65	38281.0
66	33240.0
67	28199.0
68	28199.0
69	24005.0
70	19811.0
71	16910.0
72	14009.0
73	11384.5
74	8760.0
75	8760.0
76	6770.0
77	4780.0
78	3883.5
79	2987.0
80	2278.0
81	1569.0
82	1569.0
83	1166.0
84	763.0
85	586.0
86	409.0
87	334.5
88	260.0
89	260.0
90	204.5
91	149.0
92	97.5
93	46.0
94	28.5
95	11.0
96	11.0
97	8.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1255075.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.41352740687034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.51790597640881	51.06024829077064
2	9.269063649966904	11.199536617066025
3	2.5343423284371758	4.593256791522928
4	1.0458869261255306	2.5274287390388848
5	0.5818803140535874	1.757672115029736
6	0.35667663737268135	1.2928856284382901
7	0.24119248964709125	1.0199902358538087
8	0.19077056701125233	0.9220098302846789
9	0.14479072295093212	0.7872586478331037
>10	0.933184939855443	11.075591098922306
>50	0.11449559015159105	4.880867105456424
>100	0.06795884309256574	7.326073101573058
>500	0.0013221535188332988	0.48404830454752085
>1k	5.288614075333195E-4	1.0731334936626968
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4673	0.37232834691153915	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3865	0.30794972412007254	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3075	0.24500527856900983	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1822	0.14517060733422307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.967651335577555E-5	0.0
4	0.0	0.0	0.0	7.967651335577555E-5	0.0
5	0.0	0.0	0.0	7.967651335577555E-5	0.0
6	0.0	0.0	0.0	7.967651335577555E-5	0.0
7	0.0	0.0	0.0	7.967651335577555E-5	0.0
8	0.0	0.0	0.0	7.967651335577555E-5	0.0
9	0.0	0.0	0.0	1.593530267115511E-4	0.0
10	0.0	0.0	0.0	1.593530267115511E-4	0.0
11	0.0	0.0	0.0	2.3902954006732667E-4	0.0
12	0.0	0.0	0.0	2.3902954006732667E-4	0.0
13	0.0	0.0	0.0	2.3902954006732667E-4	0.0
14	0.0	0.0	0.0	3.187060534231022E-4	0.0
15	0.0	0.0	0.0	3.187060534231022E-4	0.0
16	0.0	0.0	0.0	4.7805908013465333E-4	0.0
17	0.0	0.0	0.0	5.577355934904289E-4	0.0
18	0.0	0.0	0.0	6.374121068462044E-4	0.0
19	0.0	0.0	0.0	8.76441646913531E-4	0.0
20	0.0	0.0	0.0	0.0011951477003366332	0.0
21	0.0	0.0	0.0	0.001593530267115511	0.0
22	0.0	0.0	0.0	0.002469971914029042	0.0
23	0.0	0.0	0.0	0.003505766587654124	0.0
24	0.0	0.0	0.0	0.004939943828058084	0.0
25	0.0	0.0	0.0	0.005497679421548513	0.0
26	0.0	0.0	0.0	0.007011533175308248	0.0
27	0.0	0.0	0.0	0.01617433221122244	0.0
28	0.0	0.0	0.0	0.04214887556520527	0.0
29	0.0	0.0	0.0	0.07959683684241978	0.0
30	0.0	0.0	0.0	0.13760133856542436	0.0
31	0.0	0.0	0.0	0.34547736191064277	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGAT	85	1.2454948E-6	19.588236	14
GGCTACG	95	1.676417E-7	19.473684	1
CGCGTAA	105	2.2599124E-8	19.38095	10
CGCGCTT	70	1.2193107E-4	18.5	12
GTTCGCG	70	1.2193107E-4	18.5	30
CTAGTAC	60	9.23746E-4	18.5	3
CGAGCGT	100	2.8754403E-7	18.499998	6
AGTCGGT	115	6.406299E-8	17.695652	11
GCTACGA	105	4.79813E-7	17.619047	2
CGAATTA	140	1.869921E-9	17.178572	15
GTTCTAA	130	1.3933459E-8	17.076923	1
GCCTATG	65	0.0015800437	17.076923	7
GGTATCA	1765	0.0	16.875355	1
TCTTATA	995	0.0	16.733667	37
TAAGACT	125	1.6579543E-7	16.28	4
TCGCGTA	125	1.6579543E-7	16.28	9
TCGCCAT	265	0.0	16.056604	13
TCGTTAA	70	0.002592672	15.857142	28
CTCGTTA	70	0.002592672	15.857142	27
TAGCGTA	70	0.002592672	15.857142	7
>>END_MODULE