FastQCFastQC Report
Fri 10 Feb 2017
ERR1630115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544306
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT186163.4201349975932653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178113.272240247213883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121212.2268723842838405No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84551.5533541794505297No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA32710.6009487310446697No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA30040.5518954411672845No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.5482210374311508No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25840.4747329627084765No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23760.4365191638526858No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.41557506255672355No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.3674403736133719No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA16870.3099359551428792No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA13480.24765481181541266No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.2441641282660856No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.23497811892575132No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA12770.23461067855213794No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA12360.22707815089306385No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA12040.22119910491524988No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.1962131595095406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10340.18996667315811328No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8920.16387840663156386No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA7790.14311802552240835No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA7590.13944362178627462No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.1319110941272005No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA7120.13080877300636037No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA7110.13062505281955372No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7030.12915529132510023No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6760.12419484628131969No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGG6520.11978556179795925No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA6500.11941812142434585No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6030.11078327264443162No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA6000.11023211208401158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT5940.10912979096317145No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA5740.10545538722703775No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA5730.10527166704023105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC251.232772E-436.9999963
CACACAA250.005493833729.59999820
TGTACTG250.005493833729.5999985
GTTCTGC358.8633737E-426.4285724
ACCCGAT358.8633737E-426.428577
ATACACT509.079613E-625.8999984
TTGTGCC451.321962E-424.66666614
GACGAAG400.001929887823.12500216
CGAAGAG400.001929887823.12500218
ACGAAGA400.001929887823.12500217
AGACGAA502.6995706E-422.19999915
TTAGCCA1301.8189894E-1221.3461554
CTTAGCC1500.020.9666673
GTATTGG450.003823259120.55555534
TAAAGTA909.455289E-820.55555526
CCCTAAG450.003823259120.5555551
ATTGTGC450.003823259120.55555513
GGTATTG450.003823259120.55555533
GCCTATT450.003823259120.55555518
TAGTGTC450.003823259120.55555519
Produced by FastQC (version 0.11.5)