##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630115.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544306
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.632818671850025	31.0	31.0	34.0	30.0	34.0
2	31.730974856055234	31.0	31.0	34.0	30.0	34.0
3	31.715248408064582	33.0	31.0	34.0	28.0	34.0
4	35.47779925262628	37.0	35.0	37.0	33.0	37.0
5	35.46007025459944	37.0	35.0	37.0	33.0	37.0
6	35.5939104106881	37.0	35.0	37.0	33.0	37.0
7	35.5473263201214	37.0	35.0	37.0	33.0	37.0
8	35.640580849742605	37.0	35.0	37.0	33.0	37.0
9	37.22904028248816	39.0	37.0	39.0	34.0	39.0
10	37.136842143941095	39.0	37.0	39.0	33.0	39.0
11	37.257952328285924	39.0	37.0	39.0	34.0	39.0
12	37.223651769409116	39.0	37.0	39.0	34.0	39.0
13	37.267314709005596	39.0	37.0	39.0	34.0	39.0
14	38.244897171811445	40.0	38.0	41.0	33.0	41.0
15	38.206823000297625	40.0	37.0	41.0	33.0	41.0
16	38.04475423750611	40.0	37.0	41.0	33.0	41.0
17	38.23699536657689	40.0	38.0	41.0	33.0	41.0
18	38.31960698577638	40.0	38.0	41.0	34.0	41.0
19	38.40602896165025	40.0	38.0	41.0	34.0	41.0
20	38.453408560625824	40.0	38.0	41.0	34.0	41.0
21	38.42501276855298	40.0	38.0	41.0	34.0	41.0
22	38.347051107281565	40.0	38.0	41.0	34.0	41.0
23	38.22883451587894	40.0	37.0	41.0	34.0	41.0
24	38.13922499476397	40.0	37.0	41.0	33.0	41.0
25	38.075622903293365	40.0	37.0	41.0	33.0	41.0
26	37.73328789320713	40.0	37.0	41.0	33.0	41.0
27	37.49351100300199	40.0	36.0	41.0	33.0	41.0
28	37.26491348616403	39.0	35.0	41.0	32.0	41.0
29	37.03464595282801	39.0	35.0	41.0	32.0	41.0
30	36.72513990292225	39.0	35.0	40.0	31.0	41.0
31	36.38039815838885	38.0	35.0	40.0	31.0	41.0
32	36.036898362318254	38.0	35.0	40.0	30.0	41.0
33	35.71163095758636	38.0	35.0	40.0	30.0	41.0
34	35.52073649748487	37.0	35.0	40.0	30.0	41.0
35	35.1761196826785	37.0	35.0	40.0	27.0	41.0
36	34.87247981833748	37.0	34.0	40.0	25.0	41.0
37	34.543064011787486	36.0	34.0	40.0	24.0	41.0
38	34.18144021928841	36.0	33.0	40.0	22.0	41.0
39	33.765402181860935	35.0	33.0	40.0	20.0	41.0
40	33.29878597700558	35.0	33.0	40.0	17.0	41.0
41	32.78072444544062	35.0	33.0	40.0	15.0	41.0
42	32.30816489254206	35.0	32.0	40.0	10.0	41.0
43	31.173674366992095	35.0	30.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	9.0
12	6.0
13	3.0
14	6.0
15	6.0
16	10.0
17	18.0
18	57.0
19	125.0
20	272.0
21	428.0
22	812.0
23	1333.0
24	2273.0
25	3419.0
26	4881.0
27	6768.0
28	8986.0
29	11474.0
30	14403.0
31	17431.0
32	20936.0
33	26166.0
34	33341.0
35	41723.0
36	57809.0
37	95804.0
38	103906.0
39	91894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.00809471143071	19.85629406987981	14.152884590653054	16.982726628036435
2	18.854284171036145	27.349321888790495	31.424786792723207	22.37160714745015
3	22.487534585325168	29.44465061932075	30.050192355035588	18.017622440318497
4	14.614022259537835	17.89214155272953	31.019132620254048	36.47470356747859
5	11.865017104349391	43.18085782629624	30.157117503757082	14.797007565597292
6	29.173663343780888	32.753267463522356	18.13887776361091	19.934191429085846
7	24.683909418599097	35.89543381847711	22.05487354539542	17.365783217528374
8	32.17693723750978	30.184308091404468	19.591185840317763	18.047568830767986
9	29.346911479939592	12.075744158616661	22.80004262308334	35.77730173836041
10	21.366841445804383	30.85360073194122	26.89516558700437	20.884392235250022
11	28.794281158025083	27.38680080689906	18.6606430941419	25.15827494093396
12	19.674778525314803	34.32297273959868	27.520916543267944	18.481332191818574
13	30.470911582822897	20.922606034105815	28.45292905093826	20.15355333213303
14	23.209371199288636	24.579740072679705	30.690457206056887	21.52043152197477
15	27.268852446969166	26.872016843466728	27.592751136309356	18.266379573254753
16	19.670369240831445	26.41492101869169	31.758422651964153	22.15628708851271
17	18.559229551024607	30.68127119671655	30.793156790481824	19.966342461777014
18	16.735071816221023	24.87663189455931	34.684901507607854	23.703394781611813
19	17.67755637453932	28.030005180909267	38.053227412521636	16.239211032029775
20	18.63951527265913	25.626577697104203	38.522265049439106	17.211641980797566
21	20.495089159406657	25.90766958291843	36.71372353051409	16.883517727160825
22	20.426561529727763	26.536727502544526	35.77528081630553	17.26143015142218
23	18.48280195331303	27.759201625556212	36.02881467409876	17.729181747032
24	16.171602003284917	30.48671151888827	35.669457988704885	17.672228489121927
25	17.38452267658266	28.98296178987555	35.75268323332831	17.879832300213483
26	17.31121832204679	29.59199420913971	36.02863095391196	17.068156514901546
27	17.04831473472642	29.96917175265384	35.55867471606045	17.423838796559288
28	16.710453311188928	30.419653650703832	36.947599328319	15.922293709788244
29	16.699613820167333	30.355535305508297	36.598898413759905	16.34595246056446
30	15.76282458764004	32.352390015910174	36.25332074237653	15.63146465407326
31	16.315822349928165	32.12604674576433	35.4254775806256	16.1326533236819
32	15.267514964009216	32.3231785062079	35.62297678144279	16.786329748340087
33	15.074976208235807	32.954624788262485	35.903701226883406	16.066697776618298
34	15.400712099444064	33.16296348010127	34.618027359610224	16.81829706084445
35	15.377379635719613	33.1089497451801	35.04186983057324	16.47180078852704
36	14.739135706753187	34.07991093245344	33.74756111452014	17.433392246273236
37	15.714322458323077	33.485392407947	34.217517352371644	16.582767781358278
38	15.254838271119553	33.39040907136794	33.98290667381951	17.371845983692996
39	15.311240368469207	33.33713021719401	34.41850723673816	16.93312217759863
40	15.12255973661874	33.44001352180575	34.69482239769541	16.742604343880096
41	14.504157587827434	34.37257719003649	34.33252618931263	16.79073903282345
42	14.702207949205043	33.96324861383119	34.41170958982631	16.922833847137454
43	14.216084334914553	33.705489191741414	33.805249253177436	18.2731772201666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	877.0
1	729.0
2	581.0
3	1596.0
4	2611.0
5	2611.0
6	3750.0
7	4889.0
8	4593.5
9	4298.0
10	5964.0
11	7630.0
12	7630.0
13	14910.5
14	22191.0
15	32182.5
16	42174.0
17	34704.5
18	27235.0
19	27235.0
20	29945.5
21	32656.0
22	22225.0
23	11794.0
24	11893.5
25	11993.0
26	11993.0
27	12006.0
28	12019.0
29	12624.5
30	13230.0
31	14147.0
32	15064.0
33	15064.0
34	16655.0
35	18246.0
36	18580.0
37	18914.0
38	20272.0
39	21630.0
40	21630.0
41	24479.5
42	27329.0
43	28360.0
44	29391.0
45	32472.5
46	35554.0
47	35554.0
48	37906.5
49	40259.0
50	34485.0
51	28711.0
52	26076.5
53	23442.0
54	23442.0
55	21177.5
56	18913.0
57	16529.5
58	14146.0
59	12667.5
60	11189.0
61	11189.0
62	10549.5
63	9910.0
64	9689.0
65	9468.0
66	8336.5
67	7205.0
68	7205.0
69	5566.5
70	3928.0
71	3443.5
72	2959.0
73	3208.5
74	3458.0
75	3458.0
76	3182.0
77	2906.0
78	2692.5
79	2479.0
80	2049.0
81	1619.0
82	1619.0
83	1558.5
84	1498.0
85	1184.0
86	870.0
87	811.0
88	752.0
89	752.0
90	497.5
91	243.0
92	138.0
93	33.0
94	22.0
95	11.0
96	11.0
97	5.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	544306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.092411344896117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32889617338684	19.595642072516785
2	11.214075558192127	6.076326957505732
3	4.425985545566655	3.5973186302116877
4	2.463513278420488	2.669700603703258
5	1.6709673826144775	2.263526783684792
6	1.1113017142828636	1.8064705902983738
7	0.8300994610882958	1.574257723988649
8	0.6317242277950208	1.3691946108767648
9	0.5429702547818263	1.3239336141533058
>10	4.111160593930994	21.888087076602755
>50	0.40267178809420556	7.502389287195708
>100	0.23738590210283403	11.652733449266393
>500	0.01564434311852488	2.6580483169215685
>1k	0.010883021299843395	5.517882747752532
>5k	6.801888312402122E-4	1.5580836466702621
>10k+	0.002040566493720636	8.946403888651435
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18616	3.4201349975932653	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17811	3.272240247213883	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12121	2.2268723842838405	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8455	1.5533541794505297	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3271	0.6009487310446697	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	3004	0.5518954411672845	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2984	0.5482210374311508	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2584	0.4747329627084765	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2376	0.4365191638526858	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2262	0.41557506255672355	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2000	0.3674403736133719	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1687	0.3099359551428792	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	1348	0.24765481181541266	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1329	0.2441641282660856	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1279	0.23497811892575132	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	1277	0.23461067855213794	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1236	0.22707815089306385	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	1204	0.22119910491524988	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1068	0.1962131595095406	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1034	0.18996667315811328	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	892	0.16387840663156386	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	779	0.14311802552240835	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	759	0.13944362178627462	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	718	0.1319110941272005	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	712	0.13080877300636037	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	711	0.13062505281955372	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	703	0.12915529132510023	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	676	0.12419484628131969	No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGG	652	0.11978556179795925	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	650	0.11941812142434585	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	603	0.11078327264443162	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	600	0.11023211208401158	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	594	0.10912979096317145	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	574	0.10545538722703775	No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA	573	0.10527166704023105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.8372018680668593E-4	0.0	0.0	0.0
4	0.0	1.8372018680668593E-4	0.0	0.0	0.0
5	0.0	1.8372018680668593E-4	0.0	0.0	0.0
6	0.0	1.8372018680668593E-4	0.0	0.0	0.0
7	0.0	1.8372018680668593E-4	0.0	0.0	0.0
8	0.0	1.8372018680668593E-4	0.0	1.8372018680668593E-4	0.0
9	0.0	1.8372018680668593E-4	0.0	1.8372018680668593E-4	0.0
10	0.0	1.8372018680668593E-4	0.0	5.511605604200579E-4	0.0
11	0.0	1.8372018680668593E-4	0.0	9.186009340334297E-4	0.0
12	0.0	1.8372018680668593E-4	0.0	0.0027558028021002893	0.0
13	0.0	1.8372018680668593E-4	0.0	0.003306963362520347	0.0
14	0.0	1.8372018680668593E-4	0.0	0.004593004670167149	0.0
15	0.0	1.8372018680668593E-4	0.0	0.005695325791007264	0.0
16	0.0	1.8372018680668593E-4	0.0	0.010655770834787784	0.0
17	0.0	1.8372018680668593E-4	0.0	0.01818829849386191	0.0
18	0.0	1.8372018680668593E-4	0.0	0.0209441012959622	0.0
19	0.0	1.8372018680668593E-4	0.0	0.028292908768229635	0.0
20	0.0	1.8372018680668593E-4	0.0	0.03306963362520347	0.0
21	0.0	1.8372018680668593E-4	0.0	0.04390912464679794	0.0
22	0.0	1.8372018680668593E-4	0.0	0.06448578556914676	0.0
23	0.0	1.8372018680668593E-4	0.0	0.09847402012838366	0.0
24	0.0	1.8372018680668593E-4	0.0	0.1409133832807281	0.0
25	0.0	1.8372018680668593E-4	0.0	0.15359007617038944	0.0
26	0.0	1.8372018680668593E-4	0.0	0.17600393896080513	0.0
27	0.0	1.8372018680668593E-4	0.0	0.2028270862345813	0.0
28	0.0	1.8372018680668593E-4	0.0	0.25004317424389955	0.0
29	0.0	1.8372018680668593E-4	0.0	0.3281242536367411	0.0
30	0.0	1.8372018680668593E-4	0.0	0.4580144257090681	0.0
31	0.0	1.8372018680668593E-4	0.0	0.7929363262576565	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAC	25	1.232772E-4	36.999996	3
CACACAA	25	0.0054938337	29.599998	20
TGTACTG	25	0.0054938337	29.599998	5
GTTCTGC	35	8.8633737E-4	26.42857	24
ACCCGAT	35	8.8633737E-4	26.42857	7
ATACACT	50	9.079613E-6	25.899998	4
TTGTGCC	45	1.321962E-4	24.666666	14
GACGAAG	40	0.0019298878	23.125002	16
CGAAGAG	40	0.0019298878	23.125002	18
ACGAAGA	40	0.0019298878	23.125002	17
AGACGAA	50	2.6995706E-4	22.199999	15
TTAGCCA	130	1.8189894E-12	21.346155	4
CTTAGCC	150	0.0	20.966667	3
GTATTGG	45	0.0038232591	20.555555	34
TAAAGTA	90	9.455289E-8	20.555555	26
CCCTAAG	45	0.0038232591	20.555555	1
ATTGTGC	45	0.0038232591	20.555555	13
GGTATTG	45	0.0038232591	20.555555	33
GCCTATT	45	0.0038232591	20.555555	18
TAGTGTC	45	0.0038232591	20.555555	19
>>END_MODULE