FastQCFastQC Report
Fri 10 Feb 2017
ERR1630114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614400
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27140.44173177083333337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22440.365234375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15500.25227864583333337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.19645182291666669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA250.005494376629.627
AGGTCGC1150.024.13043610
ATGCGGC400.0019301723.12535
GAGGTCG1201.0913936E-1121.5833329
TCGATGG705.095546E-621.14285726
TAATACG450.003823810720.5555574
CGTGACG851.2433775E-619.58823625
ATCTCGC1205.158654E-918.511
TAGACAG500.00703124618.55
TTAGACA609.2301477E-418.54
CACGCTT500.00703124618.529
GTACTGG500.00703124618.51
TTGGCCG1351.1477823E-917.81481431
TCGTGAC953.6008623E-617.52631624
CTTATAC2550.017.41176437
TCGCCAT1502.5102054E-1017.26666613
TTCGATG650.001578802417.07692325
TCTTATA4600.016.89130437
GGTCGCC1554.0017767E-1016.70967711
TAGAACA1801.0913936E-1116.4444454
Produced by FastQC (version 0.11.5)