##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630114.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	614400
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.394169921875	31.0	30.0	31.0	27.0	34.0
2	30.652900390625	31.0	30.0	33.0	27.0	34.0
3	30.759708658854166	31.0	31.0	34.0	27.0	34.0
4	34.614619140625	35.0	35.0	37.0	32.0	37.0
5	33.80316080729167	35.0	33.0	37.0	28.0	37.0
6	33.976087239583336	35.0	33.0	37.0	30.0	37.0
7	34.031077473958334	35.0	33.0	37.0	30.0	37.0
8	34.005939127604165	35.0	33.0	37.0	30.0	37.0
9	35.576593424479164	37.0	35.0	39.0	30.0	39.0
10	35.14608723958333	37.0	34.0	39.0	30.0	39.0
11	35.402057291666665	37.0	34.0	39.0	30.0	39.0
12	35.241075846354164	37.0	34.0	39.0	30.0	39.0
13	35.379178059895835	37.0	34.0	39.0	30.0	39.0
14	36.179104817708335	38.0	35.0	40.0	30.0	41.0
15	36.12331217447917	38.0	34.0	40.0	30.0	41.0
16	36.039150390625	38.0	34.0	40.0	30.0	41.0
17	36.000812174479165	38.0	34.0	40.0	29.0	41.0
18	35.9109765625	38.0	34.0	40.0	29.0	41.0
19	35.940188802083334	38.0	34.0	40.0	29.0	41.0
20	35.910548502604165	38.0	34.0	40.0	29.0	41.0
21	35.831150716145835	38.0	34.0	40.0	29.0	41.0
22	35.7906298828125	38.0	34.0	40.0	29.0	41.0
23	35.6902978515625	38.0	34.0	40.0	28.0	41.0
24	35.616142578125	38.0	34.0	40.0	27.0	41.0
25	35.587277018229166	38.0	34.0	40.0	27.0	41.0
26	35.32678385416666	38.0	34.0	40.0	27.0	41.0
27	35.19709635416667	38.0	34.0	40.0	27.0	40.0
28	35.0684765625	37.0	33.0	39.0	27.0	40.0
29	34.970782877604165	37.0	33.0	39.0	27.0	40.0
30	34.850162760416666	37.0	33.0	39.0	26.0	40.0
31	34.7391796875	37.0	33.0	39.0	26.0	40.0
32	34.59777669270834	37.0	33.0	39.0	25.0	40.0
33	34.472607421875	37.0	33.0	39.0	25.0	40.0
34	34.36905436197917	37.0	33.0	39.0	25.0	40.0
35	34.19363444010417	37.0	32.0	39.0	24.0	40.0
36	34.071642252604164	37.0	32.0	39.0	24.0	40.0
37	33.984970703125	37.0	32.0	39.0	24.0	40.0
38	33.822685546875	37.0	32.0	39.0	24.0	40.0
39	33.71451497395833	36.0	31.0	39.0	24.0	40.0
40	33.57658528645833	36.0	31.0	39.0	23.0	40.0
41	33.43915690104166	36.0	31.0	39.0	23.0	40.0
42	33.345416666666665	36.0	31.0	39.0	22.0	40.0
43	32.076671549479165	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	1.0
10	1.0
11	6.0
12	3.0
13	3.0
14	14.0
15	25.0
16	32.0
17	93.0
18	156.0
19	342.0
20	568.0
21	974.0
22	1682.0
23	2648.0
24	4006.0
25	5855.0
26	8069.0
27	11395.0
28	14822.0
29	19408.0
30	24873.0
31	31654.0
32	39602.0
33	50221.0
34	63263.0
35	78270.0
36	89267.0
37	84972.0
38	59377.0
39	22797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.527018229166664	21.92529296875	14.323567708333334	24.22412109375
2	18.28125	22.998697916666664	35.289388020833336	23.4306640625
3	20.322265625	24.500651041666664	30.8232421875	24.353841145833332
4	16.066243489583336	17.308430989583336	34.55224609375	32.073079427083336
5	13.988932291666668	37.97314453125	34.2568359375	13.781087239583334
6	32.719075520833336	36.788248697916664	15.953450520833334	14.539225260416666
7	27.506673177083336	32.267578125	22.059733072916668	18.166015625
8	25.932779947916668	36.014973958333336	20.30224609375	17.75
9	26.006673177083332	15.276692708333334	20.745768229166668	37.970865885416664
10	16.63623046875	28.350911458333332	33.708984375	21.303873697916668
11	33.92464192708333	23.107096354166668	22.30712890625	20.6611328125
12	19.79833984375	26.929850260416664	30.518391927083332	22.75341796875
13	28.937337239583332	20.623860677083332	26.641276041666668	23.797526041666668
14	21.75048828125	22.0029296875	26.701171875	29.54541015625
15	24.117513020833332	29.03515625	23.506998697916668	23.34033203125
16	23.38037109375	28.217610677083332	25.291178385416668	23.11083984375
17	22.127115885416668	28.284505208333332	27.142252604166668	22.446126302083332
18	21.880859375	27.050455729166668	28.1904296875	22.878255208333336
19	23.001953125	26.871093750000004	28.610839843750004	21.51611328125
20	23.309407552083332	26.555013020833336	28.177571614583336	21.9580078125
21	22.94482421875	27.009440104166664	27.378092447916668	22.667643229166668
22	23.07568359375	26.825358072916668	27.62890625	22.470052083333332
23	22.643880208333332	26.96337890625	27.75732421875	22.635416666666668
24	22.940755208333332	26.989095052083336	27.633138020833332	22.43701171875
25	23.299153645833336	26.409016927083336	27.86376953125	22.428059895833332
26	22.740885416666668	27.2890625	27.4296875	22.540364583333332
27	22.94775390625	27.125813802083332	27.102050781249996	22.824381510416668
28	22.57080078125	27.206054687499996	27.8876953125	22.33544921875
29	22.388346354166664	27.371093749999996	28.092610677083336	22.14794921875
30	22.474283854166664	27.928385416666668	27.474121093749996	22.123209635416664
31	22.725748697916668	27.338541666666664	27.639322916666664	22.29638671875
32	22.236165364583332	27.271647135416664	27.912923177083332	22.579264322916668
33	22.417643229166668	27.102864583333336	27.882649739583332	22.596842447916668
34	22.835286458333336	26.849934895833332	27.580403645833336	22.734375
35	22.717447916666668	26.918131510416664	27.9365234375	22.427897135416668
36	22.364095052083332	26.914225260416668	27.701171875000004	23.0205078125
37	22.897135416666668	25.779296875	28.162272135416664	23.161295572916664
38	22.371907552083332	26.199218749999996	28.667805989583332	22.761067708333332
39	22.145345052083336	26.421549479166668	28.789225260416668	22.643880208333332
40	22.08251953125	25.727701822916664	29.348632812499996	22.841145833333336
41	21.422200520833336	26.078125000000004	29.425944010416664	23.07373046875
42	21.861165364583332	25.914225260416668	29.001302083333336	23.223307291666668
43	21.464680989583336	25.875813802083332	29.189453124999996	23.470052083333336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	294.0
1	230.0
2	166.0
3	260.0
4	354.0
5	354.0
6	528.5
7	703.0
8	753.0
9	803.0
10	1181.0
11	1559.0
12	1559.0
13	2624.5
14	3690.0
15	5507.0
16	7324.0
17	6860.0
18	6396.0
19	6396.0
20	8060.0
21	9724.0
22	9885.5
23	10047.0
24	11352.5
25	12658.0
26	12658.0
27	13739.0
28	14820.0
29	17146.5
30	19473.0
31	21100.0
32	22727.0
33	22727.0
34	24838.0
35	26949.0
36	28985.0
37	31021.0
38	32863.5
39	34706.0
40	34706.0
41	37305.5
42	39905.0
43	39146.5
44	38388.0
45	39727.0
46	41066.0
47	41066.0
48	41180.0
49	41294.0
50	41893.0
51	42492.0
52	43456.5
53	44421.0
54	44421.0
55	40593.0
56	36765.0
57	33983.5
58	31202.0
59	27913.0
60	24624.0
61	24624.0
62	22656.5
63	20689.0
64	18358.0
65	16027.0
66	13886.5
67	11746.0
68	11746.0
69	10090.0
70	8434.0
71	7054.0
72	5674.0
73	4675.0
74	3676.0
75	3676.0
76	2842.0
77	2008.0
78	1669.5
79	1331.0
80	959.5
81	588.0
82	588.0
83	448.0
84	308.0
85	235.5
86	163.0
87	131.5
88	100.0
89	100.0
90	78.5
91	57.0
92	38.0
93	19.0
94	13.0
95	7.0
96	7.0
97	3.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	614400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.36493927343065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.65736177244573	56.4206076735261
2	7.2035751228334055	9.27315350665536
3	1.8900182084612578	3.649527216398612
4	0.8547537724910945	2.2006469864050024
5	0.47887282423153776	1.5411310125679583
6	0.3262763101040048	1.2600452931721982
7	0.21652135132959024	0.9755468540811154
8	0.17944156922209123	0.9239796564887205
9	0.14356516089395135	0.8316506576447532
>10	0.8960747672491699	11.380092894852167
>50	0.09948323159122657	4.483568727708909
>100	0.05304077470284014	5.799822268685729
>500	0.0	0.0
>1k	0.0010151344440724498	1.2602272518132682
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2714	0.44173177083333337	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2244	0.365234375	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.25227864583333337	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.19645182291666669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.6276041666666666E-4	0.0
12	0.0	0.0	0.0	1.6276041666666666E-4	0.0
13	0.0	0.0	0.0	1.6276041666666666E-4	0.0
14	0.0	0.0	0.0	3.255208333333333E-4	0.0
15	0.0	0.0	0.0	4.8828125E-4	0.0
16	0.0	0.0	0.0	8.138020833333334E-4	0.0
17	0.0	0.0	0.0	9.765625E-4	0.0
18	0.0	0.0	0.0	0.0011393229166666667	0.0
19	0.0	0.0	0.0	0.00146484375	0.0
20	0.0	0.0	0.0	0.00146484375	0.0
21	0.0	0.0	0.0	0.0016276041666666667	0.0
22	0.0	0.0	0.0	0.0032552083333333335	0.0
23	0.0	0.0	0.0	0.004557291666666667	0.0
24	0.0	0.0	0.0	0.007161458333333333	0.0
25	0.0	0.0	0.0	0.008951822916666666	0.0
26	0.0	0.0	0.0	0.011555989583333334	0.0
27	0.0	0.0	0.0	0.026529947916666668	0.0
28	0.0	0.0	0.0	0.06526692708333333	0.0
29	0.0	0.0	0.0	0.11588541666666667	0.0
30	0.0	0.0	0.0	0.19807942708333334	0.0
31	0.0	0.0	0.0	0.4391276041666667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACTA	25	0.0054943766	29.6	27
AGGTCGC	115	0.0	24.130436	10
ATGCGGC	40	0.00193017	23.125	35
GAGGTCG	120	1.0913936E-11	21.583332	9
TCGATGG	70	5.095546E-6	21.142857	26
TAATACG	45	0.0038238107	20.555557	4
CGTGACG	85	1.2433775E-6	19.588236	25
ATCTCGC	120	5.158654E-9	18.5	11
TAGACAG	50	0.007031246	18.5	5
TTAGACA	60	9.2301477E-4	18.5	4
CACGCTT	50	0.007031246	18.5	29
GTACTGG	50	0.007031246	18.5	1
TTGGCCG	135	1.1477823E-9	17.814814	31
TCGTGAC	95	3.6008623E-6	17.526316	24
CTTATAC	255	0.0	17.411764	37
TCGCCAT	150	2.5102054E-10	17.266666	13
TTCGATG	65	0.0015788024	17.076923	25
TCTTATA	460	0.0	16.891304	37
GGTCGCC	155	4.0017767E-10	16.709677	11
TAGAACA	180	1.0913936E-11	16.444445	4
>>END_MODULE