FastQCFastQC Report
Fri 10 Feb 2017
ERR1630113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072802
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.12975367309158634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13000.12117799929530332No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA11000.10253523017294897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG358.869007E-426.4285721
TGTAACG358.869007E-426.4285722
CGAAATA851.9754225E-923.94117724
CGGTCGA957.1431714E-921.42105320
TCGAAAT957.1431714E-921.42105323
TCTAGAC2750.020.8545443
GGTCGAA1001.2894816E-820.3521
ATTAGAC656.901582E-519.9230773
GTCTAGA3350.019.8805981
TAATACT1153.0504452E-919.3043484
TTAGTCG801.6165068E-518.55
AACACGC1156.403752E-817.69565236
TAGAACT4200.017.6190474
CTCTAGT2850.017.52631827
CTAGACC953.6059337E-617.5263163
TACACCG953.6059337E-617.5263165
CTAGACA3700.017.54
CTAGAAC4050.017.3580253
GTATTAT752.0671051E-417.2666661
GTTCTAG3550.017.1971841
Produced by FastQC (version 0.11.5)