FastQCFastQC Report
Fri 10 Feb 2017
ERR1630112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704888
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG15240.2162045601570746No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG11460.16257901964567423No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT9980.1415827762708402No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8560.12143773195174268No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG7570.1073929475320902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG251.2331411E-437.010
AGTATCG303.5980783E-430.8333349
GCGTGCT400.001930449623.1250029
CTATACC753.7365135E-722.24
CCTATAC603.7236543E-521.5833343
ATAGTAC705.097303E-621.1428573
TAGACTA450.003824361120.5555575
ATCGGAT450.003824361120.55555712
CGAACGA555.1403814E-420.18181816
TAGTACA555.1403814E-420.1818184
CTAGACT656.89729E-519.9230774
GGTATCA3250.019.9230771
GCTTAGG4150.019.1686741
TCGTTTA500.007032247718.530
AACCGCG500.007032247718.57
GGTCGTC609.2319836E-418.511
ATACTAC500.007032247718.51
TCGGATG500.007032247718.513
GTGTACA902.1498126E-618.51
TATACCA1205.164111E-918.55
Produced by FastQC (version 0.11.5)