##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630112.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704888
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19720721589813	31.0	31.0	34.0	28.0	34.0
2	31.4193020735209	31.0	31.0	34.0	28.0	34.0
3	31.540881671981932	31.0	31.0	34.0	28.0	34.0
4	35.31378318257652	37.0	35.0	37.0	33.0	37.0
5	35.09835463222526	37.0	35.0	37.0	32.0	37.0
6	35.175569735901306	37.0	35.0	37.0	32.0	37.0
7	35.16471836660576	37.0	35.0	37.0	32.0	37.0
8	35.121962638036116	37.0	35.0	37.0	32.0	37.0
9	36.76114645163487	38.0	37.0	39.0	33.0	39.0
10	36.57948213049449	38.0	35.0	39.0	32.0	39.0
11	36.689607426995494	38.0	35.0	39.0	32.0	39.0
12	36.5391707618799	38.0	35.0	39.0	32.0	39.0
13	36.588111870254565	38.0	35.0	39.0	32.0	39.0
14	37.669825560940176	39.0	37.0	40.0	33.0	41.0
15	37.63603863308781	39.0	37.0	40.0	32.0	41.0
16	37.60176652177367	39.0	37.0	40.0	32.0	41.0
17	37.52987708685635	39.0	36.0	40.0	32.0	41.0
18	37.46367791762663	39.0	36.0	40.0	32.0	41.0
19	37.47094148290225	39.0	36.0	40.0	32.0	41.0
20	37.40429543416826	39.0	36.0	40.0	32.0	41.0
21	37.35006837965748	39.0	36.0	40.0	32.0	41.0
22	37.319114525995616	39.0	36.0	40.0	32.0	41.0
23	37.256559907389544	39.0	36.0	40.0	32.0	41.0
24	37.227921315159286	39.0	36.0	40.0	31.0	41.0
25	37.182365141696266	39.0	36.0	40.0	31.0	41.0
26	36.971655071443976	39.0	36.0	40.0	31.0	41.0
27	36.85538695509074	39.0	36.0	40.0	31.0	41.0
28	36.76506338595635	39.0	36.0	40.0	30.0	41.0
29	36.68172816106956	39.0	36.0	40.0	30.0	41.0
30	36.62242937885168	39.0	36.0	40.0	30.0	41.0
31	36.50174637672935	38.0	35.0	40.0	30.0	41.0
32	36.38537469782433	38.0	35.0	40.0	30.0	41.0
33	36.34234516689176	38.0	35.0	40.0	30.0	41.0
34	36.23113742892488	38.0	35.0	40.0	30.0	41.0
35	36.13777933515679	38.0	35.0	40.0	29.0	41.0
36	36.03848554664003	38.0	35.0	40.0	29.0	41.0
37	35.97566421899649	38.0	35.0	40.0	29.0	41.0
38	35.85615019691072	38.0	35.0	40.0	28.0	41.0
39	35.73779380554074	38.0	34.0	40.0	28.0	41.0
40	35.65997435053512	38.0	34.0	40.0	27.0	41.0
41	35.52270573481177	38.0	34.0	40.0	27.0	41.0
42	35.48804632792727	38.0	34.0	40.0	27.0	41.0
43	34.16631578350036	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	2.0
15	4.0
16	13.0
17	26.0
18	66.0
19	126.0
20	296.0
21	457.0
22	852.0
23	1424.0
24	2291.0
25	3521.0
26	5105.0
27	7091.0
28	9705.0
29	13000.0
30	17052.0
31	21472.0
32	27529.0
33	35293.0
34	45183.0
35	60100.0
36	82693.0
37	121144.0
38	172465.0
39	77972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.719251852776615	19.008409846670677	12.55617913767861	26.716159162874103
2	20.082481188500868	21.223655389224955	32.703351454415454	25.990511967858726
3	20.952690356482165	22.248640918841005	29.200951073078272	27.59771765159855
4	16.586464800081714	15.617516541634982	32.44104027873932	35.35497837954399
5	15.366412820192712	36.099210087276276	33.688756227939756	14.845620864591256
6	36.86968709922711	33.65513386523817	14.688716505317156	14.786462530217568
7	30.66529718196366	29.65634256789731	20.015520196116263	19.662840054022766
8	27.54238971297568	33.75075189249924	19.31058551145714	19.396272883067947
9	26.939457048495647	13.93583094051821	17.916747057688596	41.20796495329754
10	17.077606655241684	26.9896777927841	32.50360340933595	23.429112142638264
11	37.078089001373264	21.005606564447117	19.919618435836615	21.996685998343
12	22.15997435053512	24.749747477613464	28.119644539274326	24.97063363257709
13	31.6931200417655	18.82951617845672	23.841801818161183	25.6355619616166
14	23.951464629841904	19.81619775056463	23.068061876496692	33.16427574309678
15	26.429163214581607	27.152540545448357	20.779471348639785	25.638824891330252
16	26.529746569667807	25.760120756772707	21.931285537560576	25.778847135998912
17	25.264439173315477	25.708906946919225	23.51763684443486	25.509017035330434
18	24.707471257845217	24.517653868415977	25.00780265801092	25.767072215727886
19	26.154367786088002	24.67512569372723	24.50715574672856	24.66335077345621
20	26.369153681152184	23.86464232615678	24.688035546072566	25.07816844661847
21	26.068254814949327	24.710592321049585	23.351085562529082	25.870067301472005
22	26.219626380361134	24.20129155270057	23.704333170659737	25.87474889627856
23	25.478090136305344	24.893741984542224	23.788885610196232	25.839282268956204
24	26.387738193869097	24.85160763128327	23.74746058948372	25.01319358536392
25	26.39653391744504	24.303293572879664	23.890603897356744	25.40956861231855
26	26.202886132264986	24.7090317894474	23.534944558568167	25.553137519719442
27	26.337801182599225	23.909614009601526	23.719371020644413	26.033213787154843
28	25.647904347924776	24.19448196025468	24.178167311686398	25.979446380134146
29	25.211664831859814	24.60688790275902	24.30854263372337	25.872904631657796
30	25.269404501140606	25.6100259899445	23.82520343657432	25.29536607234057
31	26.32588439581891	24.65356198431524	23.756540046078243	25.264013573787608
32	25.194498984235793	24.689596077674754	24.236616313513636	25.879288624575818
33	25.426876326451865	24.43579689255598	24.068078900477808	26.06924788051435
34	25.904824596247916	23.972886472744605	24.54787143489462	25.574417496112854
35	25.852759587338696	24.12340683910068	24.42388010577567	25.599953467784953
36	25.445177106150197	24.784079172861503	24.331808741246835	25.43893497974146
37	26.517404183359627	23.731997139971174	24.075172225942275	25.675426450726924
38	25.54193006548558	24.301165575240322	24.8605452213685	25.296359137905593
39	25.678405647422004	24.02012802033798	24.919845422251477	25.381620909988538
40	24.969356833993487	23.416486023311506	25.89815687031131	25.7160002723837
41	24.510276809932925	23.697239845195263	26.097904915390814	25.694578429481
42	25.20017364460737	23.219433441908503	25.681384844117076	25.899008069367046
43	24.676402492310835	23.00294514873285	26.13947180261261	26.1811805563437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	9.0
2	12.0
3	25.0
4	38.0
5	38.0
6	53.5
7	69.0
8	83.0
9	97.0
10	123.5
11	150.0
12	150.0
13	273.0
14	396.0
15	676.5
16	957.0
17	1038.0
18	1119.0
19	1119.0
20	1455.0
21	1791.0
22	2185.0
23	2579.0
24	3369.5
25	4160.0
26	4160.0
27	5170.0
28	6180.0
29	8769.0
30	11358.0
31	12892.5
32	14427.0
33	14427.0
34	16654.0
35	18881.0
36	21092.5
37	23304.0
38	28080.0
39	32856.0
40	32856.0
41	35860.0
42	38864.0
43	38854.5
44	38845.0
45	42899.0
46	46953.0
47	46953.0
48	50860.0
49	54767.0
50	57833.5
51	60900.0
52	65551.5
53	70203.0
54	70203.0
55	63781.0
56	57359.0
57	55085.5
58	52812.0
59	47745.0
60	42678.0
61	42678.0
62	39849.5
63	37021.0
64	32605.5
65	28190.0
66	24158.5
67	20127.0
68	20127.0
69	17017.5
70	13908.0
71	11929.5
72	9951.0
73	8005.0
74	6059.0
75	6059.0
76	4783.5
77	3508.0
78	2880.0
79	2252.0
80	1673.0
81	1094.0
82	1094.0
83	815.5
84	537.0
85	404.0
86	271.0
87	197.5
88	124.0
89	124.0
90	93.0
91	62.0
92	39.0
93	16.0
94	10.5
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	704888.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.15503186188116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61246944388324	57.73548269976184
2	7.1171328558216524	9.27434035972602
3	1.7049246876766226	3.3325326704303437
4	0.708611122798848	1.8467832113456932
5	0.4029680670103989	1.3127698622691601
6	0.232752601016124	0.9099001881084763
7	0.1666456003018853	0.7600459578118155
8	0.1302164636673127	0.67874062713482
9	0.10078280144579467	0.5909855975397356
>10	0.6526145297307657	8.384318807395061
>50	0.08751436371233622	4.010309137801915
>100	0.08096682918571255	9.917602916242846
>500	0.0019641548857973123	0.8665300610737168
>1k	4.3647886351051376E-4	0.37965790335852057
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1524	0.2162045601570746	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1146	0.16257901964567423	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	998	0.1415827762708402	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	856	0.12143773195174268	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	757	0.1073929475320902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4186650928941903E-4	0.0	0.0	0.0
3	0.0	1.4186650928941903E-4	0.0	0.0	0.0
4	0.0	1.4186650928941903E-4	0.0	0.0	0.0
5	0.0	1.4186650928941903E-4	0.0	0.0	0.0
6	0.0	1.4186650928941903E-4	0.0	0.0	0.0
7	0.0	1.4186650928941903E-4	0.0	0.0	0.0
8	0.0	1.4186650928941903E-4	0.0	0.0	0.0
9	0.0	1.4186650928941903E-4	0.0	0.0	0.0
10	0.0	1.4186650928941903E-4	0.0	0.0	0.0
11	0.0	1.4186650928941903E-4	0.0	0.0	0.0
12	0.0	1.4186650928941903E-4	0.0	0.0	0.0
13	0.0	1.4186650928941903E-4	0.0	0.0	0.0
14	0.0	1.4186650928941903E-4	0.0	0.0	0.0
15	0.0	1.4186650928941903E-4	0.0	0.0	0.0
16	0.0	1.4186650928941903E-4	0.0	1.4186650928941903E-4	0.0
17	0.0	1.4186650928941903E-4	0.0	2.8373301857883806E-4	0.0
18	0.0	1.4186650928941903E-4	0.0	4.255995278682571E-4	0.0
19	0.0	1.4186650928941903E-4	0.0	4.255995278682571E-4	0.0
20	0.0	1.4186650928941903E-4	0.0	5.674660371576761E-4	0.0
21	0.0	1.4186650928941903E-4	0.0	7.093325464470952E-4	0.0
22	0.0	1.4186650928941903E-4	0.0	0.0011349320743153522	0.0
23	0.0	1.4186650928941903E-4	0.0	0.0015605316021836094	0.0
24	0.0	1.4186650928941903E-4	0.0	0.0028373301857883807	0.0
25	0.0	1.4186650928941903E-4	0.0	0.0032629297136566376	0.0
26	0.0	1.4186650928941903E-4	0.0	0.003972262260103733	0.0
27	0.0	1.4186650928941903E-4	0.0	0.006525859427313275	0.0
28	0.0	1.4186650928941903E-4	0.0	0.018017046679756215	0.0
29	0.0	1.4186650928941903E-4	0.0	0.035608493831644175	0.0
30	0.0	1.4186650928941903E-4	0.0	0.06440739521739623	0.0
31	0.0	1.4186650928941903E-4	0.0	0.1534995630511514	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGG	25	1.2331411E-4	37.0	10
AGTATCG	30	3.5980783E-4	30.833334	9
GCGTGCT	40	0.0019304496	23.125002	9
CTATACC	75	3.7365135E-7	22.2	4
CCTATAC	60	3.7236543E-5	21.583334	3
ATAGTAC	70	5.097303E-6	21.142857	3
TAGACTA	45	0.0038243611	20.555557	5
ATCGGAT	45	0.0038243611	20.555557	12
CGAACGA	55	5.1403814E-4	20.181818	16
TAGTACA	55	5.1403814E-4	20.181818	4
CTAGACT	65	6.89729E-5	19.923077	4
GGTATCA	325	0.0	19.923077	1
GCTTAGG	415	0.0	19.168674	1
TCGTTTA	50	0.0070322477	18.5	30
AACCGCG	50	0.0070322477	18.5	7
GGTCGTC	60	9.2319836E-4	18.5	11
ATACTAC	50	0.0070322477	18.5	1
TCGGATG	50	0.0070322477	18.5	13
GTGTACA	90	2.1498126E-6	18.5	1
TATACCA	120	5.164111E-9	18.5	5
>>END_MODULE