FastQCFastQC Report
Fri 10 Feb 2017
ERR1630111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440184
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142973.247959944023408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105362.3935445177471237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85271.9371444668593134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46691.0606928011922288No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA23670.5377296766806607No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA17350.3941533540519419No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15370.3491721643676281No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.3235010813659742No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12400.2817003798411573No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA10310.23422023517438162No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9700.22036239390800214No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.2165003725714701No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA7680.17447249332097486No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA7020.1594787634262036No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6830.1551623866383149No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.15357214255856644No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6710.15243625393017465No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.13267179179615796No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.12699234865419914No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA5160.11722370645002998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5010.1138160405648547No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4990.11336168511349798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT200.001840702737.029
ACTGTGC200.001840702737.08
CGATATT200.001840702737.032
TTTTGGC401.5971E-632.37521
GGAGTTG352.3849854E-531.7142858
GAGTTGA352.3849854E-531.7142859
AGCTCTC250.005492704529.622
GTCCAGA250.005492704529.611
AATACCC502.7143324E-729.65
TGGCGTA454.0008727E-628.77777924
CCGAAAT358.860667E-426.4285724
TTTGGCG509.074456E-625.90000222
GTATGTA451.3214003E-424.6666681
GCAACTG551.8992025E-523.54545431
ATGTAGG551.8992025E-523.5454543
ACAGTTG400.001929304923.1253
CAGTAAG400.001929304923.12526
CGTCCCA400.001929304923.12531
GAGGCAC400.001929304923.1257
CTTGGAC1054.0017767E-1122.904763
Produced by FastQC (version 0.11.5)