##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630111.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440184
Sequences flagged as poor quality	0
Sequence length	43
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4103306798975	31.0	31.0	34.0	30.0	34.0
2	31.525936880940698	31.0	31.0	34.0	30.0	34.0
3	31.56704241862494	31.0	31.0	34.0	28.0	34.0
4	35.351446213401665	37.0	35.0	37.0	33.0	37.0
5	35.31009759555095	37.0	35.0	37.0	33.0	37.0
6	35.42309806808062	37.0	35.0	37.0	33.0	37.0
7	35.397347472874976	37.0	35.0	37.0	33.0	37.0
8	35.43846209766825	37.0	35.0	37.0	33.0	37.0
9	36.968892554022865	39.0	37.0	39.0	33.0	39.0
10	36.82923959071661	39.0	37.0	39.0	32.0	39.0
11	36.97782291041928	39.0	37.0	39.0	33.0	39.0
12	36.86132162913691	39.0	37.0	39.0	33.0	39.0
13	36.950672900423456	39.0	37.0	39.0	33.0	39.0
14	37.85938153135962	40.0	37.0	41.0	33.0	41.0
15	37.82790832924413	39.0	37.0	41.0	33.0	41.0
16	37.73945440997401	39.0	37.0	41.0	32.0	41.0
17	37.892458608218384	39.0	37.0	41.0	33.0	41.0
18	37.991930647183906	40.0	37.0	41.0	33.0	41.0
19	38.06373016738455	40.0	37.0	41.0	33.0	41.0
20	38.0592275048616	40.0	37.0	41.0	33.0	41.0
21	38.041060102139106	40.0	37.0	41.0	33.0	41.0
22	38.00235583301529	40.0	37.0	41.0	33.0	41.0
23	37.94624066299547	40.0	37.0	41.0	33.0	41.0
24	37.913751976446214	40.0	37.0	41.0	33.0	41.0
25	37.872487414354	40.0	37.0	41.0	33.0	41.0
26	37.5999990912891	39.0	37.0	41.0	33.0	41.0
27	37.43202842447704	39.0	36.0	41.0	32.0	41.0
28	37.273737800556134	39.0	36.0	41.0	32.0	41.0
29	37.11249159442415	39.0	35.0	40.0	32.0	41.0
30	36.877962397542845	39.0	35.0	40.0	31.0	41.0
31	36.54917489050034	38.0	35.0	40.0	31.0	41.0
32	36.30166475837377	38.0	35.0	40.0	30.0	41.0
33	36.121260654635336	38.0	35.0	40.0	30.0	41.0
34	35.94450729694855	38.0	35.0	40.0	30.0	41.0
35	35.73971339258129	38.0	35.0	40.0	30.0	41.0
36	35.47544208785417	38.0	35.0	40.0	28.0	41.0
37	35.2431187866892	38.0	34.0	40.0	27.0	41.0
38	34.98595814477582	37.0	34.0	40.0	26.0	41.0
39	34.70082510949966	37.0	33.0	40.0	24.0	41.0
40	34.37463651563891	37.0	33.0	40.0	23.0	41.0
41	34.0024126274467	37.0	33.0	40.0	21.0	41.0
42	33.672641440852004	37.0	33.0	40.0	18.0	41.0
43	32.63626347163913	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	6.0
12	4.0
13	0.0
14	1.0
15	2.0
16	6.0
17	11.0
18	27.0
19	55.0
20	139.0
21	250.0
22	465.0
23	767.0
24	1410.0
25	2173.0
26	3291.0
27	4832.0
28	6560.0
29	8740.0
30	10939.0
31	13685.0
32	17091.0
33	21713.0
34	27642.0
35	35365.0
36	50044.0
37	77199.0
38	94617.0
39	63142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.880359122548754	20.275839194518657	14.699989095469167	19.143812587463422
2	17.90433091616233	26.23448376133617	33.39285389746106	22.46833142504044
3	20.368754884321103	28.80409101648402	31.406411864129545	19.420742235065337
4	13.265361757810371	18.09902222706868	34.269078385402466	34.36653762971848
5	11.328671646402414	43.02359922214347	32.294676771531904	13.353052359922216
6	28.440606655398653	36.34411973174854	18.07585009904949	17.13942351380332
7	25.163567962488415	34.56440942878432	22.424713301710195	17.847309307017063
8	31.075413917816185	31.855542227795645	19.47390182287407	17.595142031514094
9	28.3897188448467	12.479554004688948	22.270459625974592	36.86026752448976
10	19.74742380459081	30.776675208549154	28.605764861966815	20.870136124893225
11	30.444314195881724	27.13751522090762	19.673363866019663	22.744806717190993
12	19.923032186540173	33.208839939661594	27.92945677262236	18.93867110117587
13	30.94796721371063	21.003716627592098	26.752221798157134	21.29609436054014
14	22.629627610272067	23.672600548861386	30.086736455663992	23.611035385202552
15	26.360794576813333	28.325427548479727	25.96414226777893	19.34963560692801
16	19.749468404121913	27.09616887483416	29.816394962106756	23.33796775893717
17	18.883239736110355	30.060383839485304	30.052659796812243	21.003716627592098
18	17.553568507714957	25.994811260745504	33.39421696381513	23.057403267724403
19	18.675372117114662	27.840857459607797	36.20190647547389	17.281863947803647
20	19.18856659942206	26.540946513276264	36.483606855315045	17.786880031986623
21	20.52482598186213	26.53367682605456	34.714801083183396	18.226696108899915
22	20.788579321374698	26.965087327117747	33.59367900696073	18.652654344546825
23	19.13904185522418	28.16117805281428	34.13322610544681	18.56655398651473
24	17.72236155789397	29.791632590007815	33.58209294295113	18.903912909147085
25	18.41343619940752	28.857023426567075	34.43446376969631	18.2950766043291
26	18.83008014830162	29.02422623266634	34.11686930919797	18.028824309834068
27	17.402040964687494	30.147620086145793	33.70363302618905	18.746705922977664
28	17.398178943350963	30.2871072097123	35.3822492412264	16.93246460571034
29	18.237600639732477	30.251667484506477	34.327463060901806	17.18326881485924
30	17.31026116351344	31.525225814659326	34.44809443323701	16.716418588590226
31	17.870708612761934	30.89548915907893	34.145039347182085	17.088762880977047
32	16.137115388110427	31.395961688748343	34.33723170310598	18.12969122003526
33	16.22934954473584	31.470021627319483	34.989004598077166	17.31162422986751
34	17.057185177107755	31.147429256856224	33.69659051669302	18.098795049343003
35	17.266643040183197	31.893480907983935	33.712038602039144	17.127837449793724
36	16.80706250113589	32.0554586263926	32.52208167493594	18.61539719753558
37	16.75299420242444	31.836913654290022	33.24291659851335	18.167175544772185
38	16.761626956000217	31.734910855460445	33.202024607891246	18.30143758064809
39	16.249568362321213	31.534085745960784	33.95216545808117	18.26418043363684
40	16.638042273231193	31.64176798793232	34.180251894662234	17.539937844174254
41	15.919024771459208	31.719689947839996	33.81903931083365	18.542245969867146
42	15.449448412482052	32.08135688711993	33.8615215455355	18.60767315486251
43	15.85382476418952	31.6724369808989	33.73929992912055	18.734438325791032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	38.0
1	96.5
2	155.0
3	642.5
4	1130.0
5	1130.0
6	1718.0
7	2306.0
8	2223.0
9	2140.0
10	2856.5
11	3573.0
12	3573.0
13	7567.5
14	11562.0
15	19576.0
16	27590.0
17	22565.5
18	17541.0
19	17541.0
20	18962.0
21	20383.0
22	13894.5
23	7406.0
24	7659.0
25	7912.0
26	7912.0
27	8217.5
28	8523.0
29	9443.5
30	10364.0
31	11832.5
32	13301.0
33	13301.0
34	15467.0
35	17633.0
36	18734.0
37	19835.0
38	20766.5
39	21698.0
40	21698.0
41	24836.5
42	27975.0
43	29505.5
44	31036.0
45	33267.5
46	35499.0
47	35499.0
48	37474.0
49	39449.0
50	35483.5
51	31518.0
52	27904.0
53	24290.0
54	24290.0
55	21717.5
56	19145.0
57	16055.5
58	12966.0
59	10991.0
60	9016.0
61	9016.0
62	7709.5
63	6403.0
64	5003.5
65	3604.0
66	2955.0
67	2306.0
68	2306.0
69	1896.0
70	1486.0
71	1165.0
72	844.0
73	722.0
74	600.0
75	600.0
76	480.0
77	360.0
78	294.0
79	228.0
80	183.0
81	138.0
82	138.0
83	117.0
84	96.0
85	77.0
86	58.0
87	56.5
88	55.0
89	55.0
90	36.5
91	18.0
92	10.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	440184.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.243155664356298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.2811312230921	18.181555112903013
2	11.326539102256177	5.9448825759785455
3	4.971869847585567	3.9143266305932247
4	2.832768525524135	2.973631415056758
5	1.8843063698564793	2.472507269174086
6	1.4008426860258176	2.2057519602390316
7	1.0112030295283496	1.857601095852681
8	0.7826382610626447	1.6431118171158485
9	0.6640296475706539	1.5683610066249891
>10	5.05424250090742	26.66485596635828
>50	0.5350328614162436	9.666217575355589
>100	0.2372152168738685	10.423474672045216
>500	0.00952323863362246	1.7228564549047225
>1k	0.006060242766850656	3.1814794854319968
>5k	8.657489666929509E-4	1.93733311235297
>10k+	0.0017314979333859018	5.642053850013053
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14297	3.247959944023408	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10536	2.3935445177471237	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8527	1.9371444668593134	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4669	1.0606928011922288	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2367	0.5377296766806607	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1735	0.3941533540519419	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1537	0.3491721643676281	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1424	0.3235010813659742	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1240	0.2817003798411573	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1031	0.23422023517438162	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	970	0.22036239390800214	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.2165003725714701	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	768	0.17447249332097486	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	702	0.1594787634262036	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	683	0.1551623866383149	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	676	0.15357214255856644	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	671	0.15243625393017465	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	584	0.13267179179615796	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.12699234865419914	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	516	0.11722370645002998	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	501	0.1138160405648547	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	499	0.11336168511349798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.2717772567835268E-4	0.0
10	0.0	0.0	0.0	2.2717772567835268E-4	0.0
11	0.0	0.0	0.0	4.5435545135670536E-4	0.0
12	0.0	0.0	0.0	4.5435545135670536E-4	0.0
13	0.0	0.0	0.0	4.5435545135670536E-4	0.0
14	0.0	0.0	0.0	9.087109027134107E-4	0.0
15	0.0	0.0	0.0	0.0022717772567835267	0.0
16	0.0	0.0	0.0	0.0038620213365319956	0.0
17	0.0	0.0	0.0	0.007269687221707286	0.0
18	0.0	0.0	0.0	0.008632753575777401	0.0
19	0.0	0.0	0.0	0.011131708558239282	0.0
20	0.0	0.0	0.0	0.012494774912309398	0.0
21	0.0	0.0	0.0	0.014766552169092924	0.0
22	0.0	0.0	0.0	0.02203623939080021	0.0
23	0.0	0.0	0.0	0.03725714701124984	0.0
24	0.0	0.0	0.0	0.054749831888483	0.0
25	0.0	0.0	0.0	0.060656452756120166	0.0
26	0.0	0.0	0.0	0.07019791723461098	0.0
27	0.0	0.0	0.0	0.08087527034149355	0.0
28	0.0	0.0	0.0	0.10245715428093706	0.0
29	0.0	0.0	0.0	0.1444850335314323	0.0
30	0.0	0.0	0.0	0.2371735456082002	0.0
31	0.0	0.0	0.0	0.5263707903967432	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	20	0.0018407027	37.0	29
ACTGTGC	20	0.0018407027	37.0	8
CGATATT	20	0.0018407027	37.0	32
TTTTGGC	40	1.5971E-6	32.375	21
GGAGTTG	35	2.3849854E-5	31.714285	8
GAGTTGA	35	2.3849854E-5	31.714285	9
AGCTCTC	25	0.0054927045	29.6	22
GTCCAGA	25	0.0054927045	29.6	11
AATACCC	50	2.7143324E-7	29.6	5
TGGCGTA	45	4.0008727E-6	28.777779	24
CCGAAAT	35	8.860667E-4	26.42857	24
TTTGGCG	50	9.074456E-6	25.900002	22
GTATGTA	45	1.3214003E-4	24.666668	1
GCAACTG	55	1.8992025E-5	23.545454	31
ATGTAGG	55	1.8992025E-5	23.545454	3
ACAGTTG	40	0.0019293049	23.125	3
CAGTAAG	40	0.0019293049	23.125	26
CGTCCCA	40	0.0019293049	23.125	31
GAGGCAC	40	0.0019293049	23.125	7
CTTGGAC	105	4.0017767E-11	22.90476	3
>>END_MODULE