FastQCFastQC Report
Fri 10 Feb 2017
ERR1630110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660076
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT195240.7339639920062434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160100.601862503176601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115640.43472442140750867No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC93980.35329817644307904No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80990.30446498521094884No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT54640.2054076650441566No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT35170.13221426756228016No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG34010.12785348990028855No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT32480.12210177453576515No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31950.12010935025916553No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG31930.12003416443740705No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG29660.11150057366782001No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT29520.1109742729155107No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG28790.10822999042132632No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT27680.10405717731373089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATG500.00703725418.525
TGCATCA22800.018.01315714
GACAGGC24050.017.6153857
GCATCAG23850.017.29769515
CAGGACA26100.017.295024
CCAGGAC26850.016.949723
ACAGGCT25050.016.9121768
GCTGCAT24500.016.76326612
CTGCATC24400.016.68032813
GGACAGG27600.016.489136
TCCAGGA29200.016.029112
AAGACGG3750.015.7866685
ATCAAGC26650.015.34146330
GACGGAC3750.015.2933347
GGCCATC27150.015.26335226
GGTCTAT1353.9775477E-715.0740751
CGGACCA3800.014.6052649
CTTATAC14500.014.41724137
CATCAAG28650.014.33507829
TTTAGCG1551.211938E-714.32258126
Produced by FastQC (version 0.11.5)