FastQCFastQC Report
Fri 10 Feb 2017
ERR1630108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277185
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9630.3474213972617566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.2510958385194004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5920.21357577069466238No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.1497195014160218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2300.028.9565221
GTCTTAG509.058627E-625.9000021
CGAATTA400.001927511323.12515
TCTATAC652.6706675E-622.769233
GGGTGAC450.003818595820.55555511
GTATCAA3350.020.4328372
CTACACT851.2383462E-619.5882344
TCTACAC609.2127285E-418.53
ACCCCCG500.00702174818.55
TACACTT701.2149659E-418.55
GCACAGT852.7110631E-517.4117646
CCACACA752.0601439E-417.2666664
AATCTCG650.001575847917.07692310
TCTATTC650.001575847917.0769233
TATCAAC3900.016.6025643
ATCAACG3950.016.3924054
TCAACGC4000.016.18755
GGTGTAT1059.291956E-615.8571441
CTTGAAT700.002585843215.8571433
GCCTAGT700.002585843215.8571438
Produced by FastQC (version 0.11.5)