##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630108.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277185
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82691343326659	34.0	31.0	34.0	31.0	34.0
2	33.05643162508794	34.0	33.0	34.0	31.0	34.0
3	33.182625322438085	34.0	33.0	34.0	31.0	34.0
4	36.47330844021141	37.0	37.0	37.0	35.0	37.0
5	36.41896567274564	37.0	37.0	37.0	35.0	37.0
6	36.46575391886285	37.0	37.0	37.0	35.0	37.0
7	36.398232227573644	37.0	37.0	37.0	35.0	37.0
8	36.401150855926545	37.0	37.0	37.0	35.0	37.0
9	38.18110287353212	39.0	39.0	39.0	37.0	39.0
10	38.165719645723975	39.0	39.0	39.0	37.0	39.0
11	38.201111171239425	39.0	39.0	39.0	37.0	39.0
12	38.1505168028573	39.0	39.0	39.0	37.0	39.0
13	38.15746523080253	39.0	39.0	39.0	37.0	39.0
14	39.6125836535166	41.0	40.0	41.0	37.0	41.0
15	39.604679185381606	41.0	40.0	41.0	37.0	41.0
16	39.52145318108844	41.0	39.0	41.0	37.0	41.0
17	39.458246297599075	41.0	39.0	41.0	37.0	41.0
18	39.35604379746379	41.0	39.0	41.0	36.0	41.0
19	39.390215920774935	41.0	39.0	41.0	36.0	41.0
20	39.59901149052077	41.0	40.0	41.0	37.0	41.0
21	39.605602756281904	41.0	40.0	41.0	37.0	41.0
22	39.6284539206667	41.0	40.0	41.0	37.0	41.0
23	39.56371015747605	41.0	40.0	41.0	37.0	41.0
24	39.52996374262676	41.0	40.0	41.0	37.0	41.0
25	39.503609502678714	41.0	40.0	41.0	37.0	41.0
26	39.3711275862691	41.0	39.0	41.0	36.0	41.0
27	39.29162111946895	41.0	39.0	41.0	36.0	41.0
28	39.25512924581056	41.0	39.0	41.0	36.0	41.0
29	39.14345292854952	41.0	39.0	41.0	36.0	41.0
30	39.05904720673918	41.0	39.0	41.0	35.0	41.0
31	38.928195970200406	40.0	39.0	41.0	35.0	41.0
32	38.84128650540253	40.0	39.0	41.0	35.0	41.0
33	38.77954073993903	40.0	39.0	41.0	35.0	41.0
34	38.70525100564605	40.0	38.0	41.0	35.0	41.0
35	38.57585727943431	40.0	38.0	41.0	35.0	41.0
36	38.566441185489836	40.0	38.0	41.0	35.0	41.0
37	38.53417392716056	40.0	38.0	41.0	35.0	41.0
38	38.440676804300374	40.0	38.0	41.0	35.0	41.0
39	38.394707505817415	40.0	38.0	41.0	35.0	41.0
40	38.33804138030557	40.0	38.0	41.0	35.0	41.0
41	38.24284863899562	40.0	38.0	41.0	34.0	41.0
42	38.1665602395512	40.0	38.0	41.0	34.0	41.0
43	37.873795479553365	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	10.0
19	16.0
20	21.0
21	49.0
22	108.0
23	141.0
24	275.0
25	366.0
26	632.0
27	875.0
28	1162.0
29	1550.0
30	2053.0
31	2730.0
32	3413.0
33	4521.0
34	6085.0
35	8520.0
36	13032.0
37	23201.0
38	50599.0
39	157822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33382037267529	20.22872810577773	12.657611342605119	24.77984017894186
2	18.279849198188934	21.736024676659994	36.087811389505205	23.89631473564587
3	18.6680375922218	24.220646860400095	31.929216948969103	25.182098598409002
4	14.050183090715587	16.463372837635514	37.002723812616125	32.48372025903278
5	13.402240380972996	37.24263578476469	35.817594747190505	13.537529087071812
6	32.40326857513935	37.22892652921334	15.8940779623717	14.47372693327561
7	26.718978299691543	31.843714486714646	23.23141584140556	18.205891372188248
8	25.182820138174865	35.00910943954399	21.128127423922653	18.6799429983585
9	25.049335281490702	15.092447282500856	21.535075851867884	38.32314158414056
10	15.819398596605156	27.853960351389865	35.59824665836896	20.72839439363602
11	33.09775059977993	23.073398632682142	23.444270072334362	20.384580695203564
12	19.214603964861013	26.55338492342659	31.745585078557642	22.486426033154753
13	28.524992333639986	21.526056604794633	26.471490160001444	23.477460901563937
14	20.860436170788464	22.62748705738045	27.326514782545953	29.185561989285137
15	23.413243862402368	29.717697566607136	24.02294496455436	22.846113606436134
16	22.573732344823856	28.706459584753862	25.96605155401627	22.75375651640601
17	21.577646698053645	28.331619676389412	27.559211357035913	22.531522268521023
18	21.46328264516478	27.687284665476124	28.500459981600734	22.348972707758357
19	22.46153291123257	27.329400941609393	29.03259555892274	21.176470588235293
20	22.674747912044303	27.235240002164616	28.562873171347658	21.527138914443423
21	22.079116835326587	27.353572523765717	28.13463932030954	22.432671320598157
22	21.993253603189206	27.357540992477947	28.254054151559426	22.395151252773417
23	22.320832656889802	27.204935331998488	28.095315403070153	22.37891660804156
24	22.303876472392083	27.31929938488735	28.1663870700074	22.21043707271317
25	22.584916211194688	26.829373883868175	28.247560293666684	22.338149611270453
26	22.222342478849868	27.354654833414504	28.1090246586215	22.31397802911413
27	22.000469000847808	27.65986615437343	27.948121290834642	22.391543553944114
28	22.097516099356028	27.38026949510255	28.298068077276913	22.224146328264517
29	21.93841658098382	27.72480473330086	28.496491512888504	21.840287172826812
30	22.199613976225265	27.90230351570251	27.966520554864076	21.931561953208146
31	22.319750347241012	27.542976712304057	28.21148330537367	21.925789635081262
32	21.81250789184119	27.54441979183578	28.600754009055322	22.04231830726771
33	21.829103306455977	27.42067572199073	28.356512798311595	22.3937081732417
34	22.037267528906685	26.899002471273697	28.277504193949888	22.786225805869726
35	21.915688078359217	26.897198621859047	28.719447300539354	22.467665999242385
36	21.88033262983206	26.79185381604344	28.643685625124014	22.684127929000486
37	22.097516099356028	25.678157187437993	29.121705720006496	23.102620993199487
38	21.642946046864008	26.13922109782275	29.92117178057976	22.296661074733482
39	21.139311290293485	26.277035193102076	30.027238126161226	22.556415390443206
40	20.986705629814022	25.476126052997095	30.715947832674928	22.82122048451395
41	20.743907498602017	25.604560131320238	30.49335281490701	23.158179555170737
42	20.338041380305572	25.824990529790576	30.263542399480492	23.573425690423363
43	20.072875516351896	25.81524974295146	30.79892490574887	23.31294983494778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	289.0
1	209.0
2	129.0
3	262.0
4	395.0
5	395.0
6	445.0
7	495.0
8	443.5
9	392.0
10	522.0
11	652.0
12	652.0
13	1054.0
14	1456.0
15	1845.0
16	2234.0
17	2481.0
18	2728.0
19	2728.0
20	3440.0
21	4152.0
22	4452.0
23	4752.0
24	5628.5
25	6505.0
26	6505.0
27	7139.0
28	7773.0
29	8805.0
30	9837.0
31	10734.0
32	11631.0
33	11631.0
34	12591.5
35	13552.0
36	14698.0
37	15844.0
38	16458.0
39	17072.0
40	17072.0
41	17860.0
42	18648.0
43	18311.0
44	17974.0
45	18084.5
46	18195.0
47	18195.0
48	18070.5
49	17946.0
50	18092.5
51	18239.0
52	18109.0
53	17979.0
54	17979.0
55	16610.5
56	15242.0
57	14250.5
58	13259.0
59	11985.5
60	10712.0
61	10712.0
62	9679.0
63	8646.0
64	7621.0
65	6596.0
66	5730.5
67	4865.0
68	4865.0
69	4147.5
70	3430.0
71	2866.0
72	2302.0
73	1871.5
74	1441.0
75	1441.0
76	1139.5
77	838.0
78	671.5
79	505.0
80	374.5
81	244.0
82	244.0
83	188.5
84	133.0
85	94.5
86	56.0
87	42.5
88	29.0
89	29.0
90	19.5
91	10.0
92	8.0
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	277185.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.21425031825724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.47327021496223	69.85825733489422
2	6.067244305312983	9.36955441064915
3	1.402935230092661	3.249797761100296
4	0.629489702059095	1.9442230171022454
5	0.333159657324847	1.2862336588312768
6	0.20439574488990983	0.9469358525949687
7	0.13118216306219424	0.7090392663182293
8	0.1026159609808442	0.6338731598258737
9	0.08325305175943704	0.5785489780480798
>10	0.5282320567100619	7.727454985782714
>50	0.03293121169361994	1.7327085122406598
>100	0.0098793635080858	1.1456944753893006
>500	0.001411337644012257	0.8176785872229917
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	963	0.3474213972617566	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	696	0.2510958385194004	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	592	0.21357577069466238	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	415	0.1497195014160218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.6076988293017297E-4	0.0
5	0.0	0.0	0.0	3.6076988293017297E-4	0.0
6	0.0	0.0	0.0	3.6076988293017297E-4	0.0
7	0.0	0.0	0.0	3.6076988293017297E-4	0.0
8	0.0	0.0	0.0	7.215397658603459E-4	0.0
9	0.0	0.0	0.0	7.215397658603459E-4	0.0
10	0.0	0.0	0.0	0.0014430795317206919	0.0
11	0.0	0.0	0.0	0.0014430795317206919	0.0
12	0.0	0.0	0.0	0.0014430795317206919	0.0
13	0.0	0.0	0.0	0.0014430795317206919	0.0
14	0.0	0.0	0.0	0.0014430795317206919	0.0
15	0.0	0.0	0.0	0.0014430795317206919	0.0
16	0.0	0.0	0.0	0.001803849414650865	0.0
17	0.0	0.0	0.0	0.001803849414650865	0.0
18	0.0	0.0	0.0	0.001803849414650865	0.0
19	0.0	0.0	0.0	0.001803849414650865	0.0
20	0.0	0.0	0.0	0.002164619297581038	0.0
21	0.0	0.0	0.0	0.002164619297581038	0.0
22	0.0	0.0	0.0	0.0028861590634413838	0.0
23	0.0	0.0	0.0	0.005411548243952595	0.0
24	0.0	0.0	0.0	0.009740786839114671	0.0
25	0.0	0.0	0.0	0.01695618449771813	0.0
26	0.0	0.0	0.0	0.028140050868553493	0.0
27	0.0	0.0	0.0	0.08730631166910187	0.0
28	0.0	0.0	0.0	0.19914497537745549	0.0
29	0.0	0.0	0.0	0.32144596569078415	0.0
30	0.0	0.0	0.0	0.45348774284322746	0.0
31	0.0	0.0	0.0	0.7045835813626279	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	230	0.0	28.956522	1
GTCTTAG	50	9.058627E-6	25.900002	1
CGAATTA	40	0.0019275113	23.125	15
TCTATAC	65	2.6706675E-6	22.76923	3
GGGTGAC	45	0.0038185958	20.555555	11
GTATCAA	335	0.0	20.432837	2
CTACACT	85	1.2383462E-6	19.588234	4
TCTACAC	60	9.2127285E-4	18.5	3
ACCCCCG	50	0.007021748	18.5	5
TACACTT	70	1.2149659E-4	18.5	5
GCACAGT	85	2.7110631E-5	17.411764	6
CCACACA	75	2.0601439E-4	17.266666	4
AATCTCG	65	0.0015758479	17.076923	10
TCTATTC	65	0.0015758479	17.076923	3
TATCAAC	390	0.0	16.602564	3
ATCAACG	395	0.0	16.392405	4
TCAACGC	400	0.0	16.1875	5
GGTGTAT	105	9.291956E-6	15.857144	1
CTTGAAT	70	0.0025858432	15.857143	3
GCCTAGT	70	0.0025858432	15.857143	8
>>END_MODULE