FastQCFastQC Report
Fri 10 Feb 2017
ERR1630107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55719
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5400.9691487643353255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.8345447692887525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3520.6317414167519159No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.41637502467739906No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.17947199339543063No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.14537231465029882No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.13280927511261867No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAG680.12204095550889284No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.11306735583912131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTG250.00545211329.5999985
GGTATCA1800.023.638891
CCCTGTC609.0872956E-418.537
ATGAGTG500.006953219418.4999985
CTGCCTC500.006953219418.4999989
CTCTTAT1353.778532E-715.07407537
GCTGTCT1353.778532E-715.07407536
GTATCAA3200.013.2968752
TATCAAC3850.011.0519483
TCTCTTA1851.2961364E-511.036
CAACGCA3900.010.9102566
ATCAACG3900.010.9102564
TCAACGC3900.010.9102565
CTGTCTC4250.010.88235237
AACGCAG4100.010.3780487
CGCAGAG4251.8189894E-1210.0117649
GAGTACG2251.0261672E-59.8666671
GCAGAGT4657.2759576E-129.150537510
CAGAGTA4657.2759576E-129.150537511
GTCTCTT2251.1155985E-49.04444435