##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630107.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55719
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56982357903049	33.0	31.0	34.0	31.0	34.0
2	32.81571815718157	34.0	31.0	34.0	31.0	34.0
3	32.94057682298677	34.0	31.0	34.0	31.0	34.0
4	36.288232021393064	37.0	37.0	37.0	35.0	37.0
5	36.251583840341716	37.0	37.0	37.0	35.0	37.0
6	36.32988747106014	37.0	37.0	37.0	35.0	37.0
7	36.237800391248946	37.0	37.0	37.0	35.0	37.0
8	36.26640822699618	37.0	37.0	37.0	35.0	37.0
9	38.00902744126779	39.0	38.0	39.0	35.0	39.0
10	37.96878982034853	39.0	38.0	39.0	35.0	39.0
11	38.028159155763746	39.0	38.0	39.0	35.0	39.0
12	37.92864193542598	39.0	38.0	39.0	35.0	39.0
13	37.99745149769379	39.0	38.0	39.0	35.0	39.0
14	39.32064466340027	41.0	39.0	41.0	36.0	41.0
15	39.286544984655144	41.0	39.0	41.0	36.0	41.0
16	39.202534144546746	40.0	39.0	41.0	36.0	41.0
17	39.165598808305965	40.0	39.0	41.0	36.0	41.0
18	39.0918178718211	40.0	39.0	41.0	36.0	41.0
19	39.108742080798294	40.0	39.0	41.0	36.0	41.0
20	39.32001651142339	41.0	39.0	41.0	36.0	41.0
21	39.308171359859294	41.0	39.0	41.0	36.0	41.0
22	39.32818248712289	41.0	39.0	41.0	36.0	41.0
23	39.23514420574669	41.0	39.0	41.0	36.0	41.0
24	39.16342719718588	41.0	39.0	41.0	35.0	41.0
25	39.14680809059746	41.0	39.0	41.0	35.0	41.0
26	38.937759112690465	40.0	39.0	41.0	35.0	41.0
27	38.79965541377268	40.0	38.0	41.0	35.0	41.0
28	38.76731456056282	40.0	38.0	41.0	35.0	41.0
29	38.623575441052424	40.0	38.0	41.0	35.0	41.0
30	38.468386008363396	40.0	38.0	41.0	35.0	41.0
31	38.309696871803155	40.0	38.0	41.0	34.0	41.0
32	38.16850625459897	40.0	38.0	41.0	34.0	41.0
33	37.989339363592315	40.0	37.0	41.0	34.0	41.0
34	37.89972899728997	40.0	37.0	41.0	34.0	41.0
35	37.69306699689513	40.0	37.0	41.0	33.0	41.0
36	37.64464545307705	40.0	37.0	41.0	33.0	41.0
37	37.56777759830578	40.0	37.0	41.0	33.0	41.0
38	37.40244799798992	40.0	37.0	41.0	33.0	41.0
39	37.2954647427269	40.0	37.0	41.0	33.0	41.0
40	37.15547658787846	40.0	36.0	41.0	32.0	41.0
41	36.96152120461602	40.0	36.0	41.0	31.0	41.0
42	36.83291157414885	40.0	36.0	41.0	31.0	41.0
43	36.44771083472424	40.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	1.0
19	5.0
20	12.0
21	12.0
22	20.0
23	49.0
24	99.0
25	147.0
26	201.0
27	211.0
28	369.0
29	509.0
30	636.0
31	840.0
32	946.0
33	1247.0
34	1706.0
35	2259.0
36	3469.0
37	6574.0
38	12034.0
39	24370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.157289255011754	19.43681688472514	12.618675855632727	23.78721800463038
2	19.75448231303505	22.145049265062188	34.49810657046968	23.602361851433084
3	19.068899298264505	25.565785459179097	30.456397279204577	24.90891796335182
4	13.78165437283512	17.234695525763204	36.20488522766022	32.77876487374145
5	13.237854232846965	38.02473124068989	34.946786553958255	13.79062797250489
6	30.924819181966654	37.51323605951291	15.21922503993252	16.342719718587915
7	27.09309212297421	30.669968951345144	23.835675442847144	18.401263482833503
8	26.47929790556184	33.320770293795654	20.16726789784454	20.032663902797967
9	25.203252032520325	14.659272420538775	20.847466752813222	39.29000879412768
10	18.32409052567347	26.31777311150595	33.17719269907931	22.180943663741274
11	33.1933451784849	24.02950519571421	22.170175344137547	20.606974281663344
12	19.96805398517561	28.566557188750696	29.39751251817154	22.06787630790215
13	30.619716793194424	20.53339076437122	25.474254742547426	23.372637699886933
14	22.060697428166335	22.157612304599866	26.612107180674453	29.169583086559342
15	24.08514151366679	29.051131570918358	23.715429207272205	23.148297708142646
16	22.322726538523664	26.446992946750658	27.032071645219762	24.198208869505912
17	21.40921409214092	28.363753836213856	26.97284588739927	23.25418618424595
18	21.929682872987673	26.1508641576482	27.723038819792173	24.19641414957196
19	23.43904233744324	25.860119528347603	29.48904323480321	21.211794899405948
20	22.88267915791741	26.27290511315709	29.52134819361439	21.323067535311115
21	21.540228647319587	27.064376604030944	28.96677973402251	22.428615014626967
22	21.99608751054398	26.174195516789606	29.51416931387857	22.315547658787846
23	22.39631005581579	26.748505895654983	28.918322295805737	21.936861752723487
24	20.538774924173083	27.7768804178108	28.42656903390226	23.257775624113858
25	22.27067966043899	26.391356628798075	29.70261490694377	21.635348803819163
26	21.355372494122292	27.550745706132556	29.228808844379834	21.865072955365314
27	20.92105027010535	27.98865737001741	29.03497909151277	22.055313268364472
28	21.12923778244405	27.06078716416303	30.131552971158847	21.678422082234068
29	21.454082090489777	27.705091620452627	29.228808844379834	21.612017444677758
30	20.797214594662503	27.90610025305551	29.09241012939931	22.20427502288268
31	21.76636335899783	27.523824907123245	29.322134280945463	21.38767745293347
32	21.27461009709435	27.877384734112244	29.029594931710907	21.818410237082503
33	20.99104434752957	28.370932715949674	28.679624544589817	21.95839839193094
34	22.035571349090972	26.070101760620258	29.214451084908198	22.67987580538057
35	21.297941456235755	27.148728440926796	29.795940343509397	21.757389759328056
36	21.769952798865738	27.05181356449326	28.557583589080927	22.62065004756008
37	20.944381629246756	25.96780272438486	29.941312658159696	23.146502988208688
38	20.838493153143453	26.10061199949748	30.729194709165636	22.331700138193437
39	20.43288644806978	26.260342073619412	30.76329438791077	22.543477090400042
40	20.775677955455052	26.147274717780288	30.887130063353613	22.189917263411044
41	20.361097650711606	25.17812595344496	30.888924783287568	23.57185161255586
42	19.277086810603205	26.344693910515264	30.558696315439978	23.819522963441557
43	19.72935623395969	25.251709470737094	31.448877402681312	23.570056892621906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	92.5
2	79.0
3	125.5
4	172.0
5	172.0
6	176.5
7	181.0
8	172.5
9	164.0
10	215.0
11	266.0
12	266.0
13	451.0
14	636.0
15	835.5
16	1035.0
17	946.0
18	857.0
19	857.0
20	933.5
21	1010.0
22	752.0
23	494.0
24	487.5
25	481.0
26	481.0
27	551.5
28	622.0
29	879.0
30	1136.0
31	1352.0
32	1568.0
33	1568.0
34	1851.0
35	2134.0
36	2266.5
37	2399.0
38	2726.0
39	3053.0
40	3053.0
41	3560.5
42	4068.0
43	4313.5
44	4559.0
45	4728.0
46	4897.0
47	4897.0
48	4827.0
49	4757.0
50	4690.0
51	4623.0
52	4252.0
53	3881.0
54	3881.0
55	3654.0
56	3427.0
57	2919.0
58	2411.0
59	2177.0
60	1943.0
61	1943.0
62	1697.0
63	1451.0
64	1292.0
65	1133.0
66	989.5
67	846.0
68	846.0
69	702.0
70	558.0
71	453.5
72	349.0
73	274.5
74	200.0
75	200.0
76	156.0
77	112.0
78	83.5
79	55.0
80	40.5
81	26.0
82	26.0
83	22.0
84	18.0
85	10.5
86	3.0
87	4.5
88	6.0
89	6.0
90	3.5
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	55719.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.55855273784526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.69951516194739	51.11003427915074
2	9.561924434116534	11.963603079739407
3	3.3680465903548784	6.321003607387067
4	1.5520555412112345	3.8837739370771196
5	0.9352497346300599	2.9253934923455196
6	0.5995926212812348	2.2505787971787004
7	0.4475428177984336	1.9598341678781026
8	0.3127061996155722	1.5649957824081553
9	0.27254210058237943	1.5344855435309321
>10	1.2250050205123793	12.941725443744504
>50	0.014344321083283127	0.6927618945063623
>100	0.008606592649969878	1.8826612107180674
>500	0.002868864216656626	0.9691487643353255
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.9691487643353255	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	465	0.8345447692887525	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	352	0.6317414167519159	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	232	0.41637502467739906	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100	0.17947199339543063	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.14537231465029882	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.13280927511261867	No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAG	68	0.12204095550889284	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63	0.11306735583912131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0017947199339543064	0.0
10	0.0	0.0	0.0	0.0017947199339543064	0.0
11	0.0	0.0	0.0	0.003589439867908613	0.0
12	0.0	0.0	0.0	0.003589439867908613	0.0
13	0.0	0.0	0.0	0.003589439867908613	0.0
14	0.0	0.0	0.0	0.003589439867908613	0.0
15	0.0	0.0	0.0	0.003589439867908613	0.0
16	0.0	0.0	0.0	0.003589439867908613	0.0
17	0.0	0.0	0.0	0.003589439867908613	0.0
18	0.0	0.0	0.0	0.003589439867908613	0.0
19	0.0	0.0	0.0	0.003589439867908613	0.0
20	0.0	0.0	0.0	0.003589439867908613	0.0
21	0.0	0.0	0.0	0.003589439867908613	0.0
22	0.0	0.0	0.0	0.003589439867908613	0.0
23	0.0	0.0	0.0	0.007178879735817226	0.0
24	0.0	0.0	0.0	0.010768319603725839	0.0
25	0.0	0.0	0.0	0.012563039537680145	0.0
26	0.0	0.0	0.0	0.03948383854699474	0.0
27	0.0	0.0	0.0	0.1399881548484359	0.0
28	0.0	0.0	0.0	0.2781815897629175	0.0
29	0.0	0.0	0.0	0.43073278414903354	0.0
30	0.0	0.0	0.0	0.597641738006784	0.0
31	0.0	0.0	0.0	0.9242807659864678	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACTG	25	0.005452113	29.599998	5
GGTATCA	180	0.0	23.63889	1
CCCTGTC	60	9.0872956E-4	18.5	37
ATGAGTG	50	0.0069532194	18.499998	5
CTGCCTC	50	0.0069532194	18.499998	9
CTCTTAT	135	3.778532E-7	15.074075	37
GCTGTCT	135	3.778532E-7	15.074075	36
GTATCAA	320	0.0	13.296875	2
TATCAAC	385	0.0	11.051948	3
TCTCTTA	185	1.2961364E-5	11.0	36
CAACGCA	390	0.0	10.910256	6
ATCAACG	390	0.0	10.910256	4
TCAACGC	390	0.0	10.910256	5
CTGTCTC	425	0.0	10.882352	37
AACGCAG	410	0.0	10.378048	7
CGCAGAG	425	1.8189894E-12	10.011764	9
GAGTACG	225	1.0261672E-5	9.866667	1
GCAGAGT	465	7.2759576E-12	9.1505375	10
CAGAGTA	465	7.2759576E-12	9.1505375	11
GTCTCTT	225	1.1155985E-4	9.044444	35
>>END_MODULE