FastQCFastQC Report
Fri 10 Feb 2017
ERR1630106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154140
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10310.668872453613598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.6176203451407811No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7330.4755417153237317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.26145062929804075No Hit
GGCCTAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCCGAT2230.14467367328402753No Hit
TTCTAGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCC1970.12780589074867005No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATT1950.126508369015181No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.10704554301284547No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATT1620.1050992604126119No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAA1550.10055793434540028No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.10055793434540028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGAAC200.001836994837.08
GTGGGCA250.005481768429.616
TCGGAGC250.005481768429.63
GAGCAGA250.005481768429.66
ATACGCC250.005481768429.66
TGCTAGA405.907318E-527.7500021
AGATGCA405.907318E-527.75000234
GGTATCA2250.027.1333331
ACACGTA358.8344445E-426.4285723
AGTCATG358.8344445E-426.428571
TAGACAT358.8344445E-426.428575
TTGGTGT358.8344445E-426.4285734
AGACATC358.8344445E-426.428576
CATCGTA358.8344445E-426.428579
GACATCG358.8344445E-426.428577
ACATCGT358.8344445E-426.428578
GTTGGTG358.8344445E-426.4285733
AAAACAG451.3159626E-424.66666629
AATACGC551.8888346E-523.5454545
GACACGT400.001923648923.12500222
Produced by FastQC (version 0.11.5)