FastQCFastQC Report
Fri 10 Feb 2017
ERR1630105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262937
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20100.764441672339762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.6568113274282432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11510.4377474452055055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.3415266774930877No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.20042823946420626No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.1384362033490912No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.13044949930972058No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.12854790310987044No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.11029257959130893No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.10686970643157866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3900.032.256411
ATAGCTG400.001927249323.1255
CCTATCC400.001927249323.1258
TATAGCT652.669798E-622.769234
ATAAGTG502.6944285E-422.1999995
ACGGGGT603.712232E-521.58333417
TGTATGG555.128605E-420.1818182
ACTGGCC555.128605E-420.1818188
GTATCAA6200.019.9919362
CTACACT609.211007E-418.54
CGCGCAC500.007020811618.49999821
CCCCGCC500.007020811618.49999834
CAGTGCA500.007020811618.4999989
CTTTGCG803.3701689E-416.187537
TAAACTG957.026626E-515.5789485
GAACTGG750.0040932214.86
TTATAGC750.0040932214.83
TAACACT750.0040932214.84
CGGAGAA750.0040932214.818
CTTATAC3450.013.94202837