##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630105.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262937
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046014824843976	31.0	31.0	33.0	30.0	34.0
2	31.217185105177286	31.0	31.0	34.0	30.0	34.0
3	31.285950626956268	31.0	31.0	34.0	28.0	34.0
4	35.00369289982011	35.0	35.0	37.0	32.0	37.0
5	34.57733601585171	35.0	35.0	37.0	30.0	37.0
6	34.92648048772139	35.0	35.0	37.0	32.0	37.0
7	34.90532332840186	35.0	35.0	37.0	32.0	37.0
8	34.90149351365536	35.0	35.0	37.0	32.0	37.0
9	36.33352095749172	38.0	35.0	39.0	32.0	39.0
10	35.99590776497792	37.0	35.0	39.0	31.0	39.0
11	36.29352278302407	37.0	35.0	39.0	32.0	39.0
12	36.01642218478191	37.0	35.0	39.0	32.0	39.0
13	36.19782305267041	37.0	35.0	39.0	32.0	39.0
14	37.02003141436922	38.0	36.0	40.0	32.0	41.0
15	37.013938700144905	38.0	36.0	40.0	32.0	41.0
16	36.85609480598014	38.0	36.0	40.0	31.0	41.0
17	36.767609731608715	38.0	36.0	40.0	31.0	41.0
18	36.62044139850991	38.0	36.0	40.0	31.0	41.0
19	36.66546359013756	38.0	35.0	40.0	31.0	41.0
20	36.83163267246527	38.0	36.0	40.0	31.0	41.0
21	36.76379132643941	38.0	36.0	40.0	31.0	41.0
22	36.75513526053769	38.0	36.0	40.0	31.0	41.0
23	36.63837345067449	38.0	35.0	40.0	31.0	41.0
24	36.539053081156325	38.0	35.0	40.0	30.0	41.0
25	36.49572711333894	38.0	35.0	40.0	30.0	41.0
26	36.21802561069762	38.0	35.0	40.0	30.0	41.0
27	36.0114171835839	38.0	34.0	40.0	29.0	41.0
28	35.91490737324911	38.0	34.0	40.0	29.0	41.0
29	35.71887182100655	38.0	34.0	40.0	29.0	41.0
30	35.55912252744954	38.0	34.0	40.0	28.0	41.0
31	35.35340024416495	38.0	34.0	40.0	27.0	41.0
32	35.21507433339545	38.0	34.0	40.0	27.0	41.0
33	35.049935916208064	37.0	34.0	40.0	27.0	41.0
34	34.94645485420462	37.0	33.0	40.0	27.0	41.0
35	34.72284235387184	37.0	33.0	40.0	26.0	40.0
36	34.6485393839589	37.0	33.0	39.0	25.0	40.0
37	34.539771884519865	37.0	33.0	39.0	25.0	40.0
38	34.388659640902574	37.0	33.0	39.0	24.0	40.0
39	34.28254296656613	37.0	33.0	39.0	24.0	40.0
40	34.14580679021971	37.0	33.0	39.0	24.0	40.0
41	33.93968897492555	37.0	32.0	39.0	24.0	40.0
42	33.77909537265581	37.0	32.0	39.0	23.0	40.0
43	33.45767997657234	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	0.0
12	0.0
13	0.0
14	4.0
15	3.0
16	13.0
17	26.0
18	58.0
19	110.0
20	182.0
21	326.0
22	473.0
23	834.0
24	1148.0
25	1777.0
26	2430.0
27	3532.0
28	4773.0
29	6270.0
30	7981.0
31	10567.0
32	13687.0
33	18032.0
34	23735.0
35	30857.0
36	38064.0
37	41186.0
38	37032.0
39	19835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.697243065829454	25.408367783917818	16.041485222695933	20.852903927556792
2	20.040542030980806	25.3604475596816	34.32571300349513	20.273297405842463
3	20.781403910442425	26.77257289769032	31.469135192080234	20.97688799978702
4	16.464780536782577	20.033315965421377	35.56593404503741	27.93596945275865
5	13.346162769028322	38.740078421827285	35.20006693618624	12.71369187295816
6	30.58869615154961	39.46458657397019	15.67523779460479	14.271479479875406
7	25.39543692975884	33.14482176338819	23.751316855368394	17.708424451484575
8	24.458710641712653	37.04423493080091	21.004651304304833	17.4924031231816
9	24.52602714718735	15.630360124288329	22.42019951547329	37.423413213051035
10	16.31379379851447	29.100887284786854	35.38528240605164	19.20003651064704
11	32.534029063996314	24.499404800389446	23.16410394885467	19.802462186759566
12	19.13081840897249	28.24440835637434	32.05178426771432	20.572988966938848
13	28.8502569056466	22.544944226183457	26.949421344276388	21.655377523893556
14	20.282425067601746	24.103112152340675	28.436089253319235	27.17837352673834
15	22.92906665855319	30.55256582375246	25.324697551124416	21.19366996656994
16	21.56828441794042	29.20965858741828	27.541198081669755	21.680858912971548
17	20.887893297634033	29.434807577480537	28.80499891609017	20.872300208795263
18	21.038119397422196	27.651490661261064	29.72803371149743	21.58235622981931
19	22.142186151055196	27.63437629546241	30.71990628933927	19.503531264143124
20	22.132297850815974	27.839368365806255	30.098844970468214	19.929488812909558
21	21.491079612226503	27.87207582044368	29.94709759372017	20.689746973609648
22	21.610119534337123	27.822254000007607	30.29090618665308	20.276720279002195
23	21.341614150918282	27.73021674393486	30.30573863701191	20.622430468134954
24	21.22751837892727	27.90934710596074	29.92732099324173	20.93581352187026
25	21.621529111536223	27.844312515925868	30.22815351205802	20.306004860479888
26	21.504010466385484	28.09456257582615	29.73373850009698	20.667688457691387
27	21.20317794756919	28.193825897458325	29.775193297253715	20.82780285771877
28	21.08946249481815	27.8781609282832	30.557509973872065	20.47486660302658
29	21.42034023359208	27.942815199078108	30.482206764358	20.154637802971813
30	21.47510620414776	28.045501393870016	29.8147464982106	20.664645903771625
31	21.34884021647771	27.572384259347295	30.31714821421101	20.761627309963984
32	21.113422606936265	27.79449069548979	30.31904981041086	20.773036887163084
33	21.22219390956769	27.37423793532291	30.3274168336902	21.076151321419196
34	21.380026394155255	27.02510487303042	30.516055176715334	21.07881355609899
35	21.272015730003766	26.98326975663372	30.58375200143	21.160962511932517
36	21.09935079505737	26.8642298345231	30.400057808524476	21.636361561895054
37	20.830845411638528	25.90012056119907	31.53759265527484	21.731441371887563
38	20.731582090006352	26.045782830107594	31.721667167420332	21.500967912465725
39	20.166427699410885	26.262184477650543	32.11910077318901	21.45228704974956
40	20.190387811529	25.994820051951606	32.29632953901505	21.518462597504346
41	19.857608476555217	26.099027523703395	32.333981143772085	21.7093828559693
42	19.298919513039245	26.829620783685826	31.796209738454458	22.075249964820472
43	19.607738735894912	26.135538170740517	32.004624681958035	22.252098411406536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	979.0
1	673.5
2	368.0
3	485.0
4	602.0
5	602.0
6	765.0
7	928.0
8	822.0
9	716.0
10	923.5
11	1131.0
12	1131.0
13	1873.0
14	2615.0
15	3321.5
16	4028.0
17	3956.0
18	3884.0
19	3884.0
20	4891.0
21	5898.0
22	5501.5
23	5105.0
24	5995.0
25	6885.0
26	6885.0
27	7492.5
28	8100.0
29	8989.5
30	9879.0
31	10948.0
32	12017.0
33	12017.0
34	13388.5
35	14760.0
36	15469.0
37	16178.0
38	16358.5
39	16539.0
40	16539.0
41	17007.5
42	17476.0
43	17459.5
44	17443.0
45	17006.0
46	16569.0
47	16569.0
48	16096.0
49	15623.0
50	15491.0
51	15359.0
52	14969.0
53	14579.0
54	14579.0
55	14012.0
56	13445.0
57	12083.0
58	10721.0
59	9879.5
60	9038.0
61	9038.0
62	7975.0
63	6912.0
64	5881.5
65	4851.0
66	4142.0
67	3433.0
68	3433.0
69	2925.0
70	2417.0
71	2105.5
72	1794.0
73	1583.0
74	1372.0
75	1372.0
76	986.0
77	600.0
78	484.5
79	369.0
80	275.0
81	181.0
82	181.0
83	122.0
84	63.0
85	52.0
86	41.0
87	35.5
88	30.0
89	30.0
90	18.5
91	7.0
92	3.5
93	0.0
94	1.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	262937.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.7760621571018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.8499932276029	71.43730782399425
2	5.058431096494862	7.86849702756802
3	1.1417614060878962	2.664051182654166
4	0.5200305824855154	1.6178372362794922
5	0.31714218576030173	1.2333035176166178
6	0.2117442404723546	0.9881179925031701
7	0.1560687616291865	0.8496889593677461
8	0.11351912502493966	0.7063256419167615
9	0.08499864244978918	0.5949773728599669
>10	0.5123334191911905	7.22725803808091
>50	0.024128816336962743	1.2627135347766105
>100	0.007386372348049809	1.132114409705689
>500	9.848496464066412E-4	0.5457588071145489
>1k	0.0014772744696099618	1.8720484555620458
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2010	0.764441672339762	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1727	0.6568113274282432	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1151	0.4377474452055055	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.3415266774930877	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	527	0.20042823946420626	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	364	0.1384362033490912	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.13044949930972058	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	338	0.12854790310987044	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	290	0.11029257959130893	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	281	0.10686970643157866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.8031923997003083E-4	0.0
9	0.0	0.0	0.0	3.8031923997003083E-4	0.0
10	0.0	0.0	0.0	7.606384799400617E-4	0.0
11	0.0	0.0	0.0	0.0011409577199100924	0.0
12	0.0	0.0	0.0	0.0011409577199100924	0.0
13	0.0	0.0	0.0	0.0015212769598801233	0.0
14	0.0	0.0	0.0	0.0015212769598801233	0.0
15	3.8031923997003083E-4	0.0	0.0	0.0015212769598801233	0.0
16	3.8031923997003083E-4	0.0	0.0	0.0015212769598801233	0.0
17	3.8031923997003083E-4	0.0	0.0	0.002281915439820185	0.0
18	3.8031923997003083E-4	0.0	0.0	0.002281915439820185	0.0
19	3.8031923997003083E-4	0.0	0.0	0.002281915439820185	0.0
20	3.8031923997003083E-4	0.0	0.0	0.0026622346797902157	0.0
21	0.0011409577199100924	0.0	0.0	0.0026622346797902157	0.0
22	0.0011409577199100924	0.0	0.0	0.00456383087964037	0.0
23	0.0011409577199100924	0.0	0.0	0.0064654270794905246	0.0
24	0.0011409577199100924	0.0	0.0	0.011789896439070956	0.0
25	0.0011409577199100924	0.0	0.0	0.017494685038621418	0.0
26	0.0011409577199100924	0.0	0.0	0.028523942997752315	0.0
27	0.0011409577199100924	0.0	0.0	0.09013565987289732	0.0
28	0.0015212769598801233	0.0	0.0	0.2536729330600106	0.0
29	0.0015212769598801233	0.0	0.0	0.44573414924487614	0.0
30	0.0015212769598801233	0.0	0.0	0.7104363402640176	0.0
31	0.0015212769598801233	0.0	0.0	1.2052316714650277	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	390	0.0	32.25641	1
ATAGCTG	40	0.0019272493	23.125	5
CCTATCC	40	0.0019272493	23.125	8
TATAGCT	65	2.669798E-6	22.76923	4
ATAAGTG	50	2.6944285E-4	22.199999	5
ACGGGGT	60	3.712232E-5	21.583334	17
TGTATGG	55	5.128605E-4	20.181818	2
ACTGGCC	55	5.128605E-4	20.181818	8
GTATCAA	620	0.0	19.991936	2
CTACACT	60	9.211007E-4	18.5	4
CGCGCAC	50	0.0070208116	18.499998	21
CCCCGCC	50	0.0070208116	18.499998	34
CAGTGCA	50	0.0070208116	18.499998	9
CTTTGCG	80	3.3701689E-4	16.1875	37
TAAACTG	95	7.026626E-5	15.578948	5
GAACTGG	75	0.00409322	14.8	6
TTATAGC	75	0.00409322	14.8	3
TAACACT	75	0.00409322	14.8	4
CGGAGAA	75	0.00409322	14.8	18
CTTATAC	345	0.0	13.942028	37
>>END_MODULE