FastQCFastQC Report
Fri 10 Feb 2017
ERR1630104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652905
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66551.0192907084491618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55340.8475965109778605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42420.6497116732143268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34980.5357594136972453No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.5002259134177254No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28720.4398802275981957No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.3270000995550654No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.29131343763640954No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.18272183548908338No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.1569906801142586No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA6930.10614101592115238No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC6850.10491572280806548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.032.307971
CCGTGCT705.0963554E-621.1428579
GTATCAA21200.020.9433962
AGAGTGT851.243623E-619.5882366
GTCCCAT609.2309905E-418.51
CGGTCGG500.007031705718.537
ATAGTAT701.218174E-418.56
GCTTTAT4100.017.5975631
CTCTATG852.7201988E-517.4117642
AGACCGT752.065543E-417.2666666
GTCTTAC752.065543E-417.2666661
CCTCTAG1107.794897E-716.8181821
TATTGAG3350.016.5671635
GTATAAT803.3797673E-416.18751
GTTTAAG700.002590885915.8571421
GAATAGA700.002590885915.8571421
GATATCC700.002590885915.85714223
ATTGAGC3500.015.8571426
CTAGGTA2055.456968E-1215.3414644
TTATTGA4050.015.0740744
Produced by FastQC (version 0.11.5)