FastQCFastQC Report
Fri 10 Feb 2017
ERR1630103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325580
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17080.5246022482953498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.4189446526199398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10200.31328705694452974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.19442226180969346No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.1084218932366853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.027.751
CATCGTT400.001928230923.12500232
CGTTGTA450.003820008420.55555535
TAGGACT450.003820008420.5555554
CCACGAC450.003820008420.5555559
GTATAGA656.882726E-519.9230771
GTATCAA6800.018.7720582
TCGTTGT609.217441E-418.534
CTATACA500.007024318418.54
AACCACG609.217441E-418.57
TATGCTA500.007024318418.55
GCACAAT650.001576647617.0769231
GGATTCT650.001576647617.0769236
GTGCAGG1251.6468948E-716.2799991
TTATGCT803.3732576E-416.18754
GTATAAT700.00258714615.8571421
TTACCCT700.00258714615.8571424
TTTACTC957.0348586E-515.5789483
CTTGCAC855.3495E-415.2352933
ATCAACG8700.014.8850584
Produced by FastQC (version 0.11.5)