##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630103.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325580
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78432336138584	34.0	31.0	34.0	31.0	34.0
2	33.00913139627741	34.0	33.0	34.0	31.0	34.0
3	33.13602801154862	34.0	33.0	34.0	31.0	34.0
4	36.43236992444253	37.0	37.0	37.0	35.0	37.0
5	36.389206953744086	37.0	37.0	37.0	35.0	37.0
6	36.442431967565575	37.0	37.0	37.0	35.0	37.0
7	36.37655261379692	37.0	37.0	37.0	35.0	37.0
8	36.37819276368327	37.0	37.0	37.0	35.0	37.0
9	38.145217765218995	39.0	39.0	39.0	37.0	39.0
10	38.12347195773696	39.0	39.0	39.0	37.0	39.0
11	38.17068923152528	39.0	39.0	39.0	37.0	39.0
12	38.09041095890411	39.0	39.0	39.0	37.0	39.0
13	38.11389827384974	39.0	39.0	39.0	37.0	39.0
14	39.54138153449229	41.0	39.0	41.0	37.0	41.0
15	39.527821119233366	41.0	39.0	41.0	37.0	41.0
16	39.43878309478469	41.0	39.0	41.0	37.0	41.0
17	39.37916333927146	41.0	39.0	41.0	36.0	41.0
18	39.2723969531298	41.0	39.0	41.0	36.0	41.0
19	39.29536519442226	41.0	39.0	41.0	36.0	41.0
20	39.508544750906076	41.0	39.0	41.0	37.0	41.0
21	39.516969715584494	41.0	39.0	41.0	37.0	41.0
22	39.52455617666933	41.0	39.0	41.0	37.0	41.0
23	39.442947969777016	41.0	39.0	41.0	36.0	41.0
24	39.40992382824498	41.0	39.0	41.0	36.0	41.0
25	39.38536765157565	41.0	39.0	41.0	36.0	41.0
26	39.234139074881746	41.0	39.0	41.0	36.0	41.0
27	39.13855273665459	41.0	39.0	41.0	35.0	41.0
28	39.08905952454082	41.0	39.0	41.0	35.0	41.0
29	38.96670250015357	40.0	39.0	41.0	35.0	41.0
30	38.86252226795258	40.0	39.0	41.0	35.0	41.0
31	38.720584802506295	40.0	38.0	41.0	35.0	41.0
32	38.623106456170525	40.0	38.0	41.0	35.0	41.0
33	38.52701640149886	40.0	38.0	41.0	35.0	41.0
34	38.437950734074576	40.0	38.0	41.0	35.0	41.0
35	38.30179372197309	40.0	38.0	41.0	34.0	41.0
36	38.26734750291787	40.0	38.0	41.0	34.0	41.0
37	38.203151299219854	40.0	38.0	41.0	34.0	41.0
38	38.09611155476381	40.0	38.0	41.0	34.0	41.0
39	38.02840469316297	40.0	38.0	41.0	34.0	41.0
40	37.95111800479145	40.0	38.0	41.0	34.0	41.0
41	37.835625652681365	40.0	38.0	41.0	33.0	41.0
42	37.72643897045273	40.0	38.0	41.0	33.0	41.0
43	37.418723508815035	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	4.0
16	1.0
17	4.0
18	5.0
19	10.0
20	42.0
21	67.0
22	131.0
23	222.0
24	345.0
25	564.0
26	894.0
27	1155.0
28	1628.0
29	2102.0
30	2782.0
31	3597.0
32	4489.0
33	5939.0
34	8063.0
35	11404.0
36	17066.0
37	30273.0
38	61863.0
39	172928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57116530499417	19.435469009152897	12.237238159592112	24.756127526260826
2	19.181767921862523	21.574728177406474	34.86055654524234	24.382947355488664
3	19.627126973401314	23.12642054180232	31.040297315559922	26.20615516923644
4	14.240432458996253	16.365870139443455	35.84894649548498	33.54475090607531
5	14.170710731617422	36.68130720560231	34.95546409484612	14.192517967934148
6	33.3874930892561	36.0762331838565	15.440444744763193	15.09582898212421
7	27.744333189999388	30.900239572455312	22.365010135757725	18.99041710178758
8	26.193869402297437	33.96523127956262	20.34891578106763	19.4919835370723
9	25.267829719270225	14.790527673690029	21.027704404447448	38.9139382025923
10	16.63799987714233	27.205602309724185	34.54174089317525	21.61465691995823
11	34.2349038638737	22.58523250813932	22.360402973155598	20.819460654831378
12	20.278272621168377	26.102033294428406	31.01449720498802	22.6051968794152
13	30.041771607592604	20.982247066773144	25.099514712205913	23.87646661342834
14	21.62264266846858	22.15523066527428	26.793414828920696	29.428711837336447
15	24.25978254192518	28.77664475704896	23.494379261625404	23.469193439400453
16	23.056391670250015	27.46851772221881	25.685545795196262	23.78954481233491
17	22.104859020824374	27.54346090054672	27.3690030100129	22.98267706861601
18	22.344124331961424	26.688064377418762	27.909576755328953	23.058234535290868
19	23.44185760796118	26.169912156766383	28.596043983045643	21.792186252226795
20	23.414828920695374	26.19018367221574	28.17986362798698	22.215123779101912
21	22.93875545180908	26.48903495300694	27.83125499109282	22.74095460409116
22	23.076048897352415	26.37477732047423	28.067448860495116	22.481724921678236
23	23.155599238282452	26.278948338350023	27.654647091344675	22.91080533202285
24	22.778733337428587	26.64076417470361	27.681675778610483	22.898826709257325
25	22.99127710547331	26.26973401314577	27.86381227348117	22.875176607899746
26	22.799926285398367	26.82566496713557	27.604889735241724	22.769519012224336
27	22.601818293506973	26.71693592972541	27.653725658824253	23.02752011794336
28	22.86135512009337	26.498556422384667	28.047791633392716	22.592296824129246
29	22.622089808956325	26.63185699367283	28.037041587321088	22.709011610049757
30	22.72006880029486	26.638307021315804	28.020455801953435	22.6211683764359
31	22.973155599238282	26.47337060015971	27.911726764543275	22.641747036058728
32	22.451624792677684	26.585171079304626	28.129799127710548	22.833405000307145
33	22.493089256096813	26.52005651452792	28.140242029608697	22.846612199766568
34	22.555746667485717	26.07807604889735	28.11505620738375	23.251121076233183
35	22.404324589962528	26.083911788193376	28.414828920695374	23.09693470114872
36	22.57908962466982	25.887339517169362	28.22378524479391	23.309785613366916
37	22.173966459856256	25.19534369433012	28.985502795011982	23.645187050801646
38	21.922722525953684	25.46102340438602	29.470790589102524	23.145463480557773
39	21.63492843540758	25.483444929049696	29.70329872842312	23.178327907119602
40	21.586399655998527	24.95239265311137	30.154800663431413	23.30640702745869
41	21.01449720498802	25.12562196695129	30.34738006020026	23.512500767860434
42	20.685545795196266	25.406966029854416	29.934271146876345	23.973217028072977
43	20.608452607654034	25.013821487806375	30.432151852079365	23.945574052460223
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	635.0
1	460.5
2	286.0
3	472.5
4	659.0
5	659.0
6	760.5
7	862.0
8	756.0
9	650.0
10	854.0
11	1058.0
12	1058.0
13	1624.0
14	2190.0
15	2822.5
16	3455.0
17	3504.0
18	3553.0
19	3553.0
20	4335.0
21	5117.0
22	4988.0
23	4859.0
24	5623.5
25	6388.0
26	6388.0
27	7293.0
28	8198.0
29	8936.5
30	9675.0
31	10689.0
32	11703.0
33	11703.0
34	12738.5
35	13774.0
36	14674.0
37	15574.0
38	16411.0
39	17248.0
40	17248.0
41	18159.5
42	19071.0
43	19287.0
44	19503.0
45	19817.0
46	20131.0
47	20131.0
48	20345.0
49	20559.0
50	20596.0
51	20633.0
52	20488.0
53	20343.0
54	20343.0
55	20008.5
56	19674.0
57	18863.0
58	18052.0
59	17057.5
60	16063.0
61	16063.0
62	14725.5
63	13388.0
64	11875.0
65	10362.0
66	9115.5
67	7869.0
68	7869.0
69	6634.0
70	5399.0
71	4457.0
72	3515.0
73	2965.5
74	2416.0
75	2416.0
76	1856.5
77	1297.0
78	974.0
79	651.0
80	519.5
81	388.0
82	388.0
83	289.5
84	191.0
85	140.0
86	89.0
87	68.5
88	48.0
89	48.0
90	40.0
91	32.0
92	24.0
93	16.0
94	10.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	325580.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.25059066182308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77162986178148	65.401374389881
2	9.522973662052598	14.522647331769898
3	2.333688888628236	5.338354685365093
4	0.8947958256758074	2.7291484091805596
5	0.44915369112661596	1.7124117123171256
6	0.2452490617210046	1.1220231489290702
7	0.17804127805856534	0.9503026829906043
8	0.12024089856249584	0.733475162967893
9	0.0938903266074824	0.6443273575126814
>10	0.37779807828833095	4.426788580144939
>50	0.008089308062859017	0.4015462511120684
>100	0.0028312578219719187	0.5603759894505421
>500	4.0446540313884556E-4	0.19522179489388725
>1k	0.0012133962094165367	1.2620025034846551
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1708	0.5246022482953498	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1364	0.4189446526199398	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1020	0.31328705694452974	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	633	0.19442226180969346	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	353	0.1084218932366853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.071441734750292E-4	0.0	0.0	0.0
3	0.0	3.071441734750292E-4	0.0	0.0	0.0
4	0.0	3.071441734750292E-4	0.0	0.0	0.0
5	0.0	3.071441734750292E-4	0.0	0.0	0.0
6	0.0	3.071441734750292E-4	0.0	0.0	0.0
7	0.0	3.071441734750292E-4	0.0	0.0	0.0
8	0.0	3.071441734750292E-4	0.0	0.0	0.0
9	0.0	3.071441734750292E-4	0.0	3.071441734750292E-4	0.0
10	0.0	3.071441734750292E-4	0.0	6.142883469500584E-4	0.0
11	0.0	3.071441734750292E-4	0.0	6.142883469500584E-4	0.0
12	0.0	3.071441734750292E-4	0.0	9.214325204250875E-4	0.0
13	0.0	3.071441734750292E-4	0.0	9.214325204250875E-4	0.0
14	0.0	3.071441734750292E-4	0.0	9.214325204250875E-4	0.0
15	0.0	3.071441734750292E-4	0.0	9.214325204250875E-4	0.0
16	0.0	3.071441734750292E-4	0.0	9.214325204250875E-4	0.0
17	0.0	3.071441734750292E-4	0.0	0.0012285766939001167	0.0
18	0.0	3.071441734750292E-4	0.0	0.0012285766939001167	0.0
19	0.0	3.071441734750292E-4	0.0	0.0012285766939001167	0.0
20	0.0	3.071441734750292E-4	0.0	0.0012285766939001167	0.0
21	0.0	3.071441734750292E-4	0.0	0.0012285766939001167	0.0
22	0.0	3.071441734750292E-4	0.0	0.001842865040850175	0.0
23	0.0	3.071441734750292E-4	0.0	0.005528595122550525	0.0
24	0.0	3.071441734750292E-4	0.0	0.01105719024510105	0.0
25	0.0	3.071441734750292E-4	0.0	0.01873579458197678	0.0
26	0.0	3.071441734750292E-4	0.0	0.03593586829657842	0.0
27	0.0	3.071441734750292E-4	0.0	0.11333620001228577	0.0
28	0.0	9.214325204250875E-4	0.0	0.23220099514712206	0.0
29	0.0	9.214325204250875E-4	0.0	0.37010872903741016	0.0
30	0.0	9.214325204250875E-4	0.0	0.5230665274279747	0.0
31	0.0	9.214325204250875E-4	0.0	0.7896676700043	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	460	0.0	27.75	1
CATCGTT	40	0.0019282309	23.125002	32
CGTTGTA	45	0.0038200084	20.555555	35
TAGGACT	45	0.0038200084	20.555555	4
CCACGAC	45	0.0038200084	20.555555	9
GTATAGA	65	6.882726E-5	19.923077	1
GTATCAA	680	0.0	18.772058	2
TCGTTGT	60	9.217441E-4	18.5	34
CTATACA	50	0.0070243184	18.5	4
AACCACG	60	9.217441E-4	18.5	7
TATGCTA	50	0.0070243184	18.5	5
GCACAAT	65	0.0015766476	17.076923	1
GGATTCT	65	0.0015766476	17.076923	6
GTGCAGG	125	1.6468948E-7	16.279999	1
TTATGCT	80	3.3732576E-4	16.1875	4
GTATAAT	70	0.002587146	15.857142	1
TTACCCT	70	0.002587146	15.857142	4
TTTACTC	95	7.0348586E-5	15.578948	3
CTTGCAC	85	5.3495E-4	15.235293	3
ATCAACG	870	0.0	14.885058	4
>>END_MODULE