FastQCFastQC Report
Fri 10 Feb 2017
ERR1630102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394532
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41491.0516257236421886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.860766680522746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27390.6942402644145468No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20250.5132663510184219No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.365242870033356No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.2988350754818367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10870.27551630792939485No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.26639157280017844No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.16956799448460455No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.16399176746119454No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTAC4510.11431265397990531No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCT4140.10493445398598847No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAA4060.10290673506838481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8050.032.1739121
ACGTTAC303.5952317E-430.83333426
ACTCGGA303.5952317E-430.83333431
ACGGATG250.005492022329.68
CGGAATG358.85903E-426.4285714
TACGGAT358.85903E-426.428577
CGTTACT358.85903E-426.4285727
TCGGACT358.85903E-426.4285733
CCGACGT358.85903E-426.4285723
CCGGGGA451.3210613E-424.6666669
CTATCCG400.001928951923.1259
GACGTTA400.001928951923.12525
TTACTCG400.001928951923.12529
TATCCGG400.001928951923.12510
CCCGACG400.001928951923.12522
CCCCGAC502.697745E-422.221
GCCTAAG603.718306E-521.5833341
TAGACGT450.003821422320.5555554
CTCGGAC450.003821422320.55555532
CCTAAGC555.13487E-420.1818182
Produced by FastQC (version 0.11.5)