FastQCFastQC Report
Fri 10 Feb 2017
ERR1630101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335021
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16370.4886260861259443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14020.41848122953486494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10090.30117515021446417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.21670283355371753No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5530.16506427955262507No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT3640.10864990552831015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3750.027.1333311
ACGACCA451.32048E-424.66666611
CGACCAA551.8974459E-523.54545412
GCACCGA551.8974459E-523.5454546
TGCACCG652.6734342E-622.769235
ATCCTAC502.6965686E-422.19999925
TACAACC855.153197E-821.7647064
CCACGAC555.1326444E-420.1818189
GTATCAA5100.019.9509812
CTCCGTC609.218204E-418.59
AACCACG609.218204E-418.57
ACCGAGA801.6111051E-518.58
CCGAGAG801.6111051E-518.59
ACCACGA609.218204E-418.58
CACCGAG801.6111051E-518.57
ATAGGAT500.007024736618.4999983
TATGGAC500.007024736618.4999983
CTAAGTC500.007024736618.4999984
TATAGGA500.007024736618.4999982
ATTACTC752.0617622E-417.2666663
Produced by FastQC (version 0.11.5)