##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630101.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335021
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.203348446813784	33.0	31.0	34.0	31.0	34.0
2	32.48387414520284	34.0	31.0	34.0	31.0	34.0
3	32.62854865814382	34.0	31.0	34.0	31.0	34.0
4	36.1735324054313	37.0	35.0	37.0	35.0	37.0
5	36.038224469510865	37.0	35.0	37.0	35.0	37.0
6	36.117547855209075	37.0	35.0	37.0	35.0	37.0
7	36.056035293309975	37.0	35.0	37.0	35.0	37.0
8	36.05277281125661	37.0	35.0	37.0	35.0	37.0
9	37.70733476408942	39.0	37.0	39.0	35.0	39.0
10	37.605362051931074	39.0	37.0	39.0	35.0	39.0
11	37.69741001310366	39.0	37.0	39.0	35.0	39.0
12	37.58121132705114	39.0	37.0	39.0	35.0	39.0
13	37.62381761143331	39.0	37.0	39.0	35.0	39.0
14	38.885243014617	40.0	38.0	41.0	36.0	41.0
15	38.85657018515257	40.0	38.0	41.0	36.0	41.0
16	38.75752564764597	40.0	38.0	41.0	35.0	41.0
17	38.69310879019524	40.0	38.0	41.0	35.0	41.0
18	38.52391939609756	40.0	38.0	41.0	34.0	41.0
19	38.56987770915853	40.0	38.0	41.0	34.0	41.0
20	38.769626381629806	40.0	38.0	41.0	35.0	41.0
21	38.76184477987947	40.0	38.0	41.0	35.0	41.0
22	38.76854883723707	40.0	38.0	41.0	35.0	41.0
23	38.68949110652765	40.0	38.0	41.0	35.0	41.0
24	38.61762695472821	40.0	38.0	41.0	35.0	41.0
25	38.59024061178255	40.0	38.0	41.0	34.0	41.0
26	38.38477587972097	40.0	38.0	41.0	34.0	41.0
27	38.261264219257896	40.0	38.0	41.0	34.0	41.0
28	38.198918873742244	40.0	38.0	41.0	34.0	41.0
29	38.029625008581554	40.0	38.0	41.0	34.0	41.0
30	37.921566110781114	40.0	38.0	41.0	33.0	41.0
31	37.782147984753195	40.0	37.0	41.0	33.0	41.0
32	37.68742257948009	40.0	37.0	41.0	33.0	41.0
33	37.56870763325284	40.0	37.0	41.0	33.0	41.0
34	37.471274935004075	40.0	37.0	41.0	33.0	41.0
35	37.2922772005337	40.0	37.0	41.0	32.0	41.0
36	37.26222833792509	40.0	37.0	41.0	32.0	41.0
37	37.19014330444957	40.0	36.0	41.0	32.0	41.0
38	37.07313869876814	40.0	36.0	41.0	31.0	41.0
39	36.99283328507765	39.0	36.0	41.0	31.0	41.0
40	36.89720644377517	39.0	36.0	40.0	31.0	41.0
41	36.75640929971554	39.0	36.0	40.0	31.0	41.0
42	36.64018673456291	39.0	36.0	40.0	30.0	41.0
43	36.31048202948472	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	4.0
17	16.0
18	21.0
19	40.0
20	78.0
21	154.0
22	248.0
23	381.0
24	621.0
25	880.0
26	1270.0
27	1775.0
28	2487.0
29	3204.0
30	4118.0
31	5213.0
32	6989.0
33	9029.0
34	12168.0
35	17301.0
36	27305.0
37	49361.0
38	99065.0
39	93290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24362054915961	19.985314353428592	12.89292313019184	24.878141967219968
2	19.099698227872285	21.575065443658755	35.26525202897729	24.059984299491674
3	19.378486721727892	23.843281465937956	31.315947358523793	25.46228445381036
4	14.594607502216277	16.367332197086153	36.00072831255354	33.037331988144025
5	13.920918390190465	37.34183827282469	34.785580605394884	13.951662731589959
6	33.32567212204608	36.359810280549574	15.498431441611125	14.816086155793219
7	27.610507998006096	31.085812531154765	22.563063210962895	18.740616259876248
8	25.962253112491457	34.67931860987819	20.37305124156396	18.985377036066396
9	25.388259243450413	14.782058438127759	20.842872536348466	38.986809782073365
10	16.76103886025055	27.275006641374723	34.279045194181855	21.68490930419287
11	34.0121962503843	22.822748424725614	22.44098131161927	20.72407401327081
12	20.742281827109345	25.929120861080353	30.89806310649183	22.430534205318473
13	29.28085105112814	21.02614462973963	25.708239185006317	23.984765134125922
14	21.220759295685944	22.292930890899378	27.04875216777456	29.437557645640123
15	24.08654979837085	28.811626733846534	23.74358622295319	23.35823724482943
16	22.812301318424815	27.89049044686751	25.36826049710317	23.92894773760451
17	21.972652460592023	27.846612600404153	27.473501661089898	22.70723327791392
18	22.275021565812292	26.486697848791568	27.988991734846476	23.249288850549668
19	23.09825354231526	26.182537811062588	28.561194671378807	22.158013975243342
20	23.130191838720556	26.58251273800747	28.218828073464053	22.06846734980792
21	23.381220878691185	26.509382993901877	27.66722086078186	22.442175266625078
22	23.154966405091024	26.545201644076045	27.950486685909244	22.34934526492369
23	23.117356822408148	26.685192868506753	27.444548252199112	22.752902056885986
24	22.829016688506094	26.548186531590556	27.768707036275337	22.854089743628013
25	23.054972673354804	26.419537879715005	27.916757456995235	22.60873198993496
26	22.954083475364232	26.682207980992235	28.08182173654786	22.281886807095674
27	23.018557045677735	26.849958659307926	27.459472689771687	22.672011605242655
28	22.705442345405213	26.490876691311886	28.15077263813313	22.652908325149767
29	22.45530877168894	26.457744439900782	28.525674509956094	22.561272278454187
30	22.5857483560732	26.70429614859964	28.152862059393293	22.55709343593387
31	22.647535527623642	26.629972449488243	28.172562316989087	22.549929705899032
32	22.238605938135223	26.477743186248027	28.468663158428875	22.81498771718788
33	22.65798263392444	26.644001420806458	28.017348166234356	22.680667779034746
34	22.906325275132005	26.232086943803523	28.19285955208778	22.668728228976693
35	22.727530513012617	26.300142379134446	28.337925085293158	22.63440202255978
36	22.86692475994042	26.288202829076386	27.883326716832673	22.961545694150516
37	22.181296097856553	25.370349918363328	29.013703618579136	23.434650365200987
38	22.39770044265882	25.623468379594115	29.430990893108195	22.547840284638873
39	21.491787082003817	25.733909217631133	29.614859964002253	23.159443736362796
40	21.621629688885175	24.86262055214449	30.60106679879769	22.914682960172648
41	21.070022476202986	25.06290650436838	30.274818593461305	23.592252425967327
42	20.731237743305645	25.49899857023888	29.996030099605697	23.77373358684978
43	20.72884983329403	24.750687270350216	30.640168825237822	23.88029407111793
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	519.0
1	375.5
2	232.0
3	363.0
4	494.0
5	494.0
6	562.5
7	631.0
8	595.0
9	559.0
10	744.0
11	929.0
12	929.0
13	1510.5
14	2092.0
15	2762.5
16	3433.0
17	3488.0
18	3543.0
19	3543.0
20	4485.5
21	5428.0
22	5364.0
23	5300.0
24	6070.0
25	6840.0
26	6840.0
27	7658.0
28	8476.0
29	9423.0
30	10370.0
31	11692.0
32	13014.0
33	13014.0
34	14306.5
35	15599.0
36	16856.5
37	18114.0
38	18647.0
39	19180.0
40	19180.0
41	19847.5
42	20515.0
43	20678.5
44	20842.0
45	20768.0
46	20694.0
47	20694.0
48	20140.0
49	19586.0
50	20193.0
51	20800.0
52	20604.0
53	20408.0
54	20408.0
55	20217.5
56	20027.0
57	18740.0
58	17453.0
59	15809.5
60	14166.0
61	14166.0
62	13282.0
63	12398.0
64	10751.0
65	9104.0
66	7795.0
67	6486.0
68	6486.0
69	5539.0
70	4592.0
71	4053.5
72	3515.0
73	3642.0
74	3769.0
75	3769.0
76	3442.5
77	3116.0
78	2391.0
79	1666.0
80	1159.0
81	652.0
82	652.0
83	440.5
84	229.0
85	181.5
86	134.0
87	102.5
88	71.0
89	71.0
90	50.5
91	30.0
92	21.0
93	12.0
94	7.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	335021.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.08190401055855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49216836865929	66.2975022678376
2	6.888909419929106	10.20687052769241
3	1.4709041741348075	3.2690214551095407
4	0.6367998060341471	1.8870136841825595
5	0.3457405306148152	1.2806558400783157
6	0.22139188979156305	0.9840679636952837
7	0.1596614798119115	0.827961849512683
8	0.11164773101781193	0.6616861193806561
9	0.08512427028360209	0.5675551218106785
>10	0.514766599484827	6.987798343741536
>50	0.04494620031359035	2.2920935534428017
>100	0.025914926306803497	3.139817199815854
>500	8.098414470876093E-4	0.38366544363852845
>1k	0.001214762170631414	1.2142906300615819
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1637	0.4886260861259443	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1402	0.41848122953486494	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1009	0.30117515021446417	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	726	0.21670283355371753	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	553	0.16506427955262507	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	364	0.10864990552831015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.9848875145140156E-4	0.0	0.0	0.0
6	0.0	2.9848875145140156E-4	0.0	0.0	0.0
7	0.0	2.9848875145140156E-4	0.0	0.0	0.0
8	0.0	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
9	0.0	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
10	0.0	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
11	0.0	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
12	0.0	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
13	2.9848875145140156E-4	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
14	2.9848875145140156E-4	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
15	2.9848875145140156E-4	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
16	2.9848875145140156E-4	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
17	2.9848875145140156E-4	2.9848875145140156E-4	0.0	2.9848875145140156E-4	0.0
18	2.9848875145140156E-4	2.9848875145140156E-4	0.0	2.9848875145140156E-4	2.9848875145140156E-4
19	2.9848875145140156E-4	2.9848875145140156E-4	0.0	2.9848875145140156E-4	2.9848875145140156E-4
20	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.0011939550058056062	2.9848875145140156E-4
21	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.0014924437572570077	2.9848875145140156E-4
22	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.004178842520319622	2.9848875145140156E-4
23	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.0074622187862850384	2.9848875145140156E-4
24	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.01343199381531307	2.9848875145140156E-4
25	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.02149119010450091	2.9848875145140156E-4
26	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.03432620641691118	2.9848875145140156E-4
27	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.10447106300799054	2.9848875145140156E-4
28	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.2325227373806418	2.9848875145140156E-4
29	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.37370791681715476	2.9848875145140156E-4
30	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.5271311350631751	2.9848875145140156E-4
31	2.9848875145140156E-4	2.9848875145140156E-4	0.0	0.8068150951731384	2.9848875145140156E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	375	0.0	27.133331	1
ACGACCA	45	1.32048E-4	24.666666	11
CGACCAA	55	1.8974459E-5	23.545454	12
GCACCGA	55	1.8974459E-5	23.545454	6
TGCACCG	65	2.6734342E-6	22.76923	5
ATCCTAC	50	2.6965686E-4	22.199999	25
TACAACC	85	5.153197E-8	21.764706	4
CCACGAC	55	5.1326444E-4	20.181818	9
GTATCAA	510	0.0	19.950981	2
CTCCGTC	60	9.218204E-4	18.5	9
AACCACG	60	9.218204E-4	18.5	7
ACCGAGA	80	1.6111051E-5	18.5	8
CCGAGAG	80	1.6111051E-5	18.5	9
ACCACGA	60	9.218204E-4	18.5	8
CACCGAG	80	1.6111051E-5	18.5	7
ATAGGAT	50	0.0070247366	18.499998	3
TATGGAC	50	0.0070247366	18.499998	3
CTAAGTC	50	0.0070247366	18.499998	4
TATAGGA	50	0.0070247366	18.499998	2
ATTACTC	75	2.0617622E-4	17.266666	3
>>END_MODULE