FastQCFastQC Report
Fri 10 Feb 2017
ERR1630100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692250
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57670.8330805344889851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44490.6426868905742145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36250.5236547490068617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27710.40028891296496927No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.25799927771758757No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.22564102564102562No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.20902853015529071No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA12800.18490429758035393No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA11870.17146984470928134No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT11060.15976886962802456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10770.15557963163596966No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC10100.14590104730949802No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.13391115926327193No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.12495485734922356No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA8030.11599855543517515No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG7590.10964247020585048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11000.034.309091
GCGCGAG250.00549485329.5999987
CCCCGAA405.9367692E-527.7512
CGAATTA358.8658166E-426.42857215
GCGAGAA855.173206E-821.76470613
CGTGCGA806.9518865E-720.812510
TATGACT450.003824293420.5555574
GTATCAA18400.020.510872
CGACCAA656.8970636E-519.92307712
AACCACG759.257041E-619.7333347
CCGTGCG951.6737067E-719.4736849
TCCGTGC951.6737067E-719.4736848
TGCGAGA902.1496999E-618.512
GCCGGCT500.00703212618.49999828
CCGAATT500.00703212618.49999814
CGAGAAT1054.790454E-717.61904714
GTATAGA953.6021938E-617.5263161
GTGCGAG953.6021938E-617.52631611
GCTTTAT4800.016.9583341
TCTACAC1107.7965706E-716.8181823
Produced by FastQC (version 0.11.5)