FastQCFastQC Report
Fri 10 Feb 2017
ERR1630099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699531
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74730.4397095433975608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61740.36327669221685277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49290.2900211881983912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.2069394438818709No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC23280.13697896655018355No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17430.10255770562584618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.031.7664471
GTATCAA22700.021.0264322
CGCGAAC902.1534488E-618.528
TATGTCG500.007036232418.510
ATACCGA609.239296E-418.56
GACAGGC9850.018.2182757
CGTGCGA1301.3944373E-817.07692310
GGACAGG11800.016.9322036
CCAGGAC12950.016.7142853
CGGTCGG803.3837574E-416.187537
CAGGACA11800.015.9915264
TGCATCA10600.015.88207514
GACCGAC700.002593177815.8571437
GCATCAG10550.015.7819915
ACAGGCT11300.015.7168148
GAACCGT957.0628535E-515.5789486
GCTGCAT11150.015.09865612
GTCTATA1853.0559022E-1015.01
ATCAAGC10900.014.9357830
CCGCGAA1405.999209E-714.53571527
Produced by FastQC (version 0.11.5)