FastQCFastQC Report
Fri 10 Feb 2017
ERR1630098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107850
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81800.38807315511065776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73090.3467514291813934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61630.2923832341010983No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46030.2183741727352516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.030.0748671
CGTGCGA502.7031385E-422.210
GTACGCA500.007036781418.527
GTATCAA30400.018.3174342
TAATACG752.0682975E-417.2666664
GTCTAGA5300.016.7547171
CTCTAGT4250.016.10588327
CTAGAAC5700.015.9035083
TCTAGAC5900.015.6779673
TAGAACT6350.015.4409444
CGAATTA2900.015.31034515
CTAGACA6550.015.2519084
GTTCTAG6000.014.81
ATGCGTT2251.8189894E-1214.836
GCAATTA5050.014.65346417
TAGACAT5500.014.4636365
TGCGTTA2353.6379788E-1214.17021437
ACTGATC5250.014.0952388
TGGACCG800.006300918813.8750015
AATGCGT2551.8189894E-1213.78431235
Produced by FastQC (version 0.11.5)