##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630098.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2107850
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23442180420808	33.0	31.0	34.0	31.0	34.0
2	32.508828427070235	34.0	31.0	34.0	31.0	34.0
3	32.675255829399624	34.0	31.0	34.0	31.0	34.0
4	36.20117892639419	37.0	35.0	37.0	35.0	37.0
5	36.07398534051284	37.0	35.0	37.0	35.0	37.0
6	36.16121735417605	37.0	35.0	37.0	35.0	37.0
7	36.09598548283796	37.0	35.0	37.0	35.0	37.0
8	36.098666888061295	37.0	35.0	37.0	35.0	37.0
9	37.76140190241241	39.0	37.0	39.0	35.0	39.0
10	37.6420252864293	39.0	37.0	39.0	35.0	39.0
11	37.747057428185116	39.0	37.0	39.0	35.0	39.0
12	37.6250715183718	39.0	37.0	39.0	35.0	39.0
13	37.68024005503238	39.0	37.0	39.0	35.0	39.0
14	38.9460236734113	40.0	38.0	41.0	36.0	41.0
15	38.919978176815235	40.0	38.0	41.0	36.0	41.0
16	38.83722987878644	40.0	38.0	41.0	36.0	41.0
17	38.76683255449866	40.0	38.0	41.0	35.0	41.0
18	38.6523618853334	40.0	38.0	41.0	35.0	41.0
19	38.680296985079586	40.0	38.0	41.0	35.0	41.0
20	38.87838745641293	40.0	38.0	41.0	35.0	41.0
21	38.8643152975781	40.0	38.0	41.0	35.0	41.0
22	38.874653319733376	40.0	38.0	41.0	35.0	41.0
23	38.8111877031098	40.0	38.0	41.0	35.0	41.0
24	38.75466565457694	40.0	38.0	41.0	35.0	41.0
25	38.725864743696185	40.0	38.0	41.0	35.0	41.0
26	38.56047109614062	40.0	38.0	41.0	35.0	41.0
27	38.43035178025002	40.0	38.0	41.0	34.0	41.0
28	38.38659060179804	40.0	38.0	41.0	34.0	41.0
29	38.24622577507887	40.0	38.0	41.0	34.0	41.0
30	38.14784258841948	40.0	38.0	41.0	34.0	41.0
31	38.00602794316484	40.0	38.0	41.0	34.0	41.0
32	37.90560760964964	40.0	38.0	41.0	33.0	41.0
33	37.81368740659914	40.0	37.0	41.0	33.0	41.0
34	37.738934933700214	40.0	37.0	41.0	33.0	41.0
35	37.59527433166497	40.0	37.0	41.0	33.0	41.0
36	37.56809687596366	40.0	37.0	41.0	33.0	41.0
37	37.51635695139597	40.0	37.0	41.0	33.0	41.0
38	37.396772540740564	40.0	37.0	41.0	33.0	41.0
39	37.34332329150556	40.0	37.0	41.0	32.0	41.0
40	37.2648376307612	40.0	37.0	41.0	32.0	41.0
41	37.136759257062884	40.0	37.0	41.0	31.0	41.0
42	37.022104039661265	39.0	36.0	40.0	31.0	41.0
43	36.70739426429775	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	3.0
15	11.0
16	14.0
17	45.0
18	80.0
19	143.0
20	285.0
21	568.0
22	1027.0
23	1840.0
24	2957.0
25	4576.0
26	6709.0
27	9395.0
28	13270.0
29	17689.0
30	23131.0
31	30130.0
32	38933.0
33	52121.0
34	71110.0
35	102546.0
36	164054.0
37	301490.0
38	639873.0
39	625846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66423132575847	20.335507744858504	12.268425172569206	24.731835756813815
2	18.465403135896764	22.32141755817539	35.551723319970584	23.661455985957254
3	19.039068244894086	24.08847878169699	31.674217804872264	25.19823516853666
4	13.824370804374125	16.198828189861707	37.43079441136704	32.546006594397134
5	13.491424911639823	37.588775292359514	35.599022700856324	13.320777095144344
6	32.72377066679318	37.62876865052067	15.472590554356335	14.174870128329815
7	27.450103185710557	31.970586142277675	22.66000901392414	17.919301658087626
8	25.802832269848423	35.27048888678037	20.634675142918137	18.29200370045307
9	25.65936855089309	14.457148279052115	21.050454254334987	38.83302891571981
10	15.374528548046587	27.797803449012026	35.58241810375501	21.245249899186376
11	34.00042697535403	22.82923357923951	23.142111630334227	20.02822781507223
12	19.97428659534597	26.5227601584553	31.94990155846004	21.55305168773869
13	29.222477880304577	21.449012026472474	26.109210807220627	23.21929928600232
14	21.136371183907773	21.941077401143342	27.60153711127452	29.32101430367436
15	23.48658585762744	29.477050074720683	24.14925160708779	22.887112460564083
16	22.828948929003488	28.051521692720073	25.85516047157056	23.264368906705883
17	21.603245012690657	28.086818321986858	27.6177147330218	22.692221932300686
18	22.317574779989087	26.795028109210804	28.309794340204476	22.577602770595632
19	22.93555044239391	26.581682757311953	28.700808881087365	21.781957919206775
20	22.91961002917665	26.94707877695282	28.56374979244254	21.569561401427993
21	22.602984083307636	26.834214958369902	28.547240078753234	22.01556087956923
22	22.33560262827051	26.814763858908364	28.672581066015134	22.177052446805988
23	22.50520672723391	26.832459615247767	28.3531560594919	22.309177598026427
24	22.262589842730744	26.940294612994283	28.191711933961145	22.605403610313825
25	22.814716417202362	27.015489717010222	28.242474559385155	21.927319306402257
26	22.538510804848542	26.88326968237778	28.058258414972602	22.51996109780108
27	22.171833859145575	27.0746495243969	28.209028156652515	22.544488459805017
28	22.361221149512538	26.810494105368026	28.43874089712266	22.389543847996773
29	22.132599568280476	27.02806176910122	28.42204141660934	22.417297246008967
30	22.166520388073156	27.28415209810945	28.374409943781583	22.174917570035817
31	22.62063239794103	27.028109210807223	28.375121569371636	21.976136821880115
32	22.214626277960956	26.963493607230117	28.68932798823446	22.132552126574474
33	22.047726356239767	26.845126550750763	28.819745238038756	22.287401854970703
34	22.37051972388927	26.273786085347627	28.694214483952845	22.661479706810255
35	21.941172284555353	26.761676589890172	28.953056431909292	22.344094693645182
36	22.33811703868871	26.3974191711934	28.785255117774035	22.479208672343855
37	22.236828996370708	25.903740778518397	28.964774533292214	22.894655691818677
38	21.87138553502384	26.125008895319873	29.4164195744479	22.587185995208387
39	21.670612235215977	26.173446877149704	29.70363166259459	22.45230922503973
40	21.5746851056764	25.94003368361126	29.89017245060132	22.595108760111014
41	21.491045377991792	25.42699907488673	30.089285290699053	22.99267025642242
42	21.020233887610598	25.849894442204143	29.851554901914273	23.278316768270987
43	20.706074910453783	25.563346537941506	30.407571696278197	23.32300685532652
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2147.0
1	1800.0
2	1453.0
3	2461.0
4	3469.0
5	3469.0
6	3994.5
7	4520.0
8	3852.5
9	3185.0
10	4389.5
11	5594.0
12	5594.0
13	8858.0
14	12122.0
15	15401.0
16	18680.0
17	19823.5
18	20967.0
19	20967.0
20	26987.5
21	33008.0
22	33859.0
23	34710.0
24	40619.0
25	46528.0
26	46528.0
27	52086.5
28	57645.0
29	64092.5
30	70540.0
31	76904.0
32	83268.0
33	83268.0
34	91468.5
35	99669.0
36	107486.5
37	115304.0
38	118036.0
39	120768.0
40	120768.0
41	126265.5
42	131763.0
43	134328.0
44	136893.0
45	139034.0
46	141175.0
47	141175.0
48	139484.5
49	137794.0
50	138122.5
51	138451.0
52	134538.5
53	130626.0
54	130626.0
55	125594.0
56	120562.0
57	112797.0
58	105032.0
59	99606.0
60	94180.0
61	94180.0
62	85319.5
63	76459.0
64	66945.5
65	57432.0
66	49199.5
67	40967.0
68	40967.0
69	33980.5
70	26994.0
71	21864.0
72	16734.0
73	13095.0
74	9456.0
75	9456.0
76	7160.5
77	4865.0
78	3637.5
79	2410.0
80	1823.0
81	1236.0
82	1236.0
83	922.0
84	608.0
85	455.0
86	302.0
87	245.5
88	189.0
89	189.0
90	139.0
91	89.0
92	59.5
93	30.0
94	22.0
95	14.0
96	14.0
97	11.5
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2107850.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.813071212793176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.68450737890484	50.054273992619514
2	9.299136872368287	11.124198719289504
3	2.654317384222367	4.762886241715421
4	1.1865515698299114	2.838851741755521
5	0.7207512801723897	2.15551738238315
6	0.45158510215520886	1.6206415124307594
7	0.3293439433872889	1.3789350917528227
8	0.24167542229431566	1.1564279395257413
9	0.17557670050195684	0.9451603521387724
>10	1.1085423827120935	12.48762996453915
>50	0.09741529689580494	4.018403930884238
>100	0.047729737613508405	4.8947834796516
>500	0.0019909228763782855	0.8318200509535492
>1k	6.370953204410463E-4	0.6991137197414631
>5k	2.3891074516539236E-4	1.0313558806188021
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8180	0.38807315511065776	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7309	0.3467514291813934	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6163	0.2923832341010983	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4603	0.2183741727352516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	4.74417060037479E-5	0.0	4.74417060037479E-5	0.0
12	0.0	4.74417060037479E-5	0.0	1.4232511801124368E-4	0.0
13	0.0	4.74417060037479E-5	0.0	1.4232511801124368E-4	0.0
14	0.0	4.74417060037479E-5	0.0	1.4232511801124368E-4	0.0
15	0.0	4.74417060037479E-5	0.0	1.897668240149916E-4	0.0
16	0.0	4.74417060037479E-5	0.0	3.3209194202623525E-4	0.0
17	0.0	4.74417060037479E-5	0.0	3.3209194202623525E-4	0.0
18	0.0	4.74417060037479E-5	0.0	4.2697535403373105E-4	0.0
19	0.0	4.74417060037479E-5	0.0	6.641838840524705E-4	0.0
20	0.0	4.74417060037479E-5	0.0	0.0010437175320824536	0.0
21	0.0	4.74417060037479E-5	0.0	0.0015655762981236804	0.0
22	0.0	4.74417060037479E-5	0.0	0.0026092938302061343	0.0
23	0.0	4.74417060037479E-5	0.0	0.00360556965628484	0.0
24	0.0	4.74417060037479E-5	0.0	0.005598121308442252	0.0
25	0.0	4.74417060037479E-5	0.0	0.008065090020637142	0.0
26	0.0	4.74417060037479E-5	0.0	0.013663211329079394	0.0
27	0.0	4.74417060037479E-5	0.0	0.055079820670351304	0.0
28	0.0	1.4232511801124368E-4	0.0	0.14004791612306378	0.0
29	0.0	1.4232511801124368E-4	0.0	0.24275920962117797	0.0
30	0.0	1.4232511801124368E-4	0.0	0.3533458263159143	0.0
31	0.0	1.4232511801124368E-4	0.0	0.56303816685248	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1870	0.0	30.074867	1
CGTGCGA	50	2.7031385E-4	22.2	10
GTACGCA	50	0.0070367814	18.5	27
GTATCAA	3040	0.0	18.317434	2
TAATACG	75	2.0682975E-4	17.266666	4
GTCTAGA	530	0.0	16.754717	1
CTCTAGT	425	0.0	16.105883	27
CTAGAAC	570	0.0	15.903508	3
TCTAGAC	590	0.0	15.677967	3
TAGAACT	635	0.0	15.440944	4
CGAATTA	290	0.0	15.310345	15
CTAGACA	655	0.0	15.251908	4
GTTCTAG	600	0.0	14.8	1
ATGCGTT	225	1.8189894E-12	14.8	36
GCAATTA	505	0.0	14.653464	17
TAGACAT	550	0.0	14.463636	5
TGCGTTA	235	3.6379788E-12	14.170214	37
ACTGATC	525	0.0	14.095238	8
TGGACCG	80	0.0063009188	13.875001	5
AATGCGT	255	1.8189894E-12	13.784312	35
>>END_MODULE