FastQCFastQC Report
Fri 10 Feb 2017
ERR1630097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712042
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53950.7576800244929371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.6425182784161608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32340.45418669123450583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.3633212647568542No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG22580.3171161251723915No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC13860.19465143910050248No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC11510.16164776796874342No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.15659188643366542No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG10660.1497102698998093No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG10610.14900806413104845No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC10340.14521615297973994No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.1421264475971923No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA10090.1417051241359358No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT9660.13566615452459263No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG9040.12695880299195833No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.126677920684454No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG8830.12400953876316285No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA8710.12232424491813686No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.1175492456905632No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA8120.11403821684675905No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC8020.11263380530923738No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.11136983492546787No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT7850.11024630569545055No Hit
CTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGA7800.10954409992668972No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA7700.10813968838916807No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT7700.10813968838916807No Hit
TTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATCAG7680.10785880608166372No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA7420.1042073360841074No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC7370.10350513031534656No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC7370.10350513031534656No Hit
TATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTCTCTTC7360.10336468916159441No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC7270.10210071877782491No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG7240.1016793953165684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7190.10097718954780759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.031.3201771
CGTTTAG604.317917E-827.75000226
GCGTTTA701.92078E-723.78571325
ATTAGAC551.9013412E-523.5454543
CGTGTAA400.001930469323.1251
AATGCGT652.6804664E-622.7692335
ATGCGTT705.097423E-621.14285736
TGCGTTA705.097423E-621.14285737
TTCGTCT806.9524685E-720.812518
ACGTCCG555.1404536E-420.1818188
GTATCAA18200.019.719782
TGCGTTT851.2439468E-619.58823424
TTTAGAC1254.110916E-1019.243
GACCGTA500.00703231718.535
ACCTAGG500.00703231718.52
CGAATTA500.00703231718.515
CGTCCGG609.23211E-418.59
ATTTTCG902.1498763E-618.49999815
GTGTAAG1156.394657E-817.6956521
GATTAAT1156.394657E-817.6956521
Produced by FastQC (version 0.11.5)