##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630097.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	712042
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494281236219212	31.0	31.0	33.0	30.0	34.0
2	31.70118616598459	31.0	31.0	34.0	30.0	34.0
3	31.83086531412473	31.0	31.0	34.0	30.0	34.0
4	35.57581856126465	37.0	35.0	37.0	33.0	37.0
5	35.3201370143896	37.0	35.0	37.0	33.0	37.0
6	35.51985416590595	37.0	35.0	37.0	33.0	37.0
7	35.46316088095927	37.0	35.0	37.0	33.0	37.0
8	35.46096157249151	37.0	35.0	37.0	33.0	37.0
9	36.98977167077223	39.0	37.0	39.0	33.0	39.0
10	36.74128351979237	38.0	37.0	39.0	32.0	39.0
11	36.946213846936	38.0	37.0	39.0	33.0	39.0
12	36.721897022928424	38.0	37.0	39.0	32.0	39.0
13	36.86835467570733	38.0	37.0	39.0	33.0	39.0
14	37.857962030329674	39.0	37.0	40.0	33.0	41.0
15	37.83208855657391	39.0	37.0	40.0	33.0	41.0
16	37.69120079995281	39.0	37.0	40.0	33.0	41.0
17	37.605132843287336	39.0	37.0	40.0	33.0	41.0
18	37.4419023035158	39.0	36.0	40.0	32.0	41.0
19	37.51285879203755	39.0	37.0	40.0	32.0	41.0
20	37.6906165647532	39.0	37.0	40.0	33.0	41.0
21	37.64466702806857	39.0	37.0	40.0	33.0	41.0
22	37.65091665941054	39.0	37.0	40.0	33.0	41.0
23	37.54735254381062	39.0	37.0	40.0	33.0	41.0
24	37.47344679106008	39.0	36.0	40.0	32.0	41.0
25	37.4471533982546	39.0	36.0	40.0	32.0	41.0
26	37.18969246196151	39.0	36.0	40.0	32.0	41.0
27	37.003679558228306	39.0	36.0	40.0	31.0	41.0
28	36.93121192289219	39.0	36.0	40.0	31.0	41.0
29	36.750080753663404	38.0	36.0	40.0	31.0	41.0
30	36.627464391145466	38.0	35.0	40.0	31.0	41.0
31	36.405429735886365	38.0	35.0	40.0	30.0	41.0
32	36.27750610216813	38.0	35.0	40.0	30.0	41.0
33	36.14483134421846	38.0	35.0	40.0	30.0	41.0
34	36.03470300909216	38.0	35.0	40.0	30.0	41.0
35	35.858933883113636	38.0	35.0	40.0	29.0	41.0
36	35.764431030753805	38.0	35.0	40.0	29.0	41.0
37	35.66136267242663	38.0	35.0	40.0	29.0	41.0
38	35.54089646397263	38.0	34.0	40.0	28.0	41.0
39	35.417346167782235	38.0	34.0	40.0	27.0	41.0
40	35.29536038604464	38.0	34.0	40.0	27.0	41.0
41	35.09672041817758	38.0	34.0	40.0	26.0	41.0
42	34.94219161229253	38.0	34.0	40.0	26.0	41.0
43	34.59868518991857	38.0	33.0	40.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	5.0
16	14.0
17	28.0
18	50.0
19	134.0
20	258.0
21	452.0
22	854.0
23	1278.0
24	2010.0
25	2928.0
26	4291.0
27	6201.0
28	7942.0
29	10933.0
30	14478.0
31	18413.0
32	24289.0
33	33449.0
34	47038.0
35	68862.0
36	102711.0
37	141408.0
38	155114.0
39	68899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04386819878603	21.934239834167084	13.471255909061544	22.550636057985344
2	20.021150437755075	23.905331427078742	33.69983793090857	22.373680204257614
3	20.827423101446264	24.286769600669622	31.215574362186498	23.670232935697612
4	14.45504619109547	17.808921383850954	36.38015735026867	31.355875074784915
5	13.734021307731847	37.62966229520169	34.8847399451156	13.751576451950868
6	32.52701385592423	37.51759025450746	15.120737259880737	14.834658629687574
7	27.745413894124223	31.819049999859562	22.01738099718837	18.41815510882785
8	25.934284775336288	34.86718480089658	20.570275348926046	18.62825507484109
9	25.047820212852614	14.282163130826554	21.430477415658064	39.239539240662765
10	16.109442982295988	28.01604399740465	35.49959131624258	20.374921704056785
11	33.58804677252184	23.303400642096957	22.20416773167875	20.904384853702453
12	19.838436496723507	26.575819965676185	31.61878091460897	21.96696262299134
13	29.74332974740254	21.469800938708673	25.949592861095272	22.837276452793514
14	20.894834855247304	23.356487398215272	27.494024228907843	28.25465351762958
15	23.491030023509847	29.52719081177796	25.314237081520474	21.66754208319172
16	22.918310998508513	27.969277093205175	26.178652382865053	22.933759525421255
17	22.005724381426937	27.717887427988796	27.57014333424152	22.706244856342746
18	21.272200235379373	26.278506043182848	29.125809994354267	23.323483727083516
19	23.43401091508647	26.73241185210985	29.617073150179348	20.21650408262434
20	23.182902132177595	26.894902267001104	28.95166296370158	20.97053263711972
21	22.05558099100896	27.010766218846644	28.856162979150106	22.077489810994294
22	22.902722030442025	26.39605528887341	29.242376151968564	21.458846528716002
23	21.11771496625199	26.577645700674964	29.76903047853919	22.535608854533862
24	21.711219282008646	27.29923234865359	29.06120706362827	21.928341305709495
25	22.39924049424051	26.997564750393938	28.869083565295306	21.734111190070248
26	22.673241185210983	27.036888273444543	28.127975596945127	22.161894944399346
27	22.463141219197745	27.05093238881976	27.944699891298548	22.541226500683948
28	21.91556116071805	27.05008974189725	29.041404860949214	21.99294423643549
29	22.154451563250483	27.685726403779555	29.458936411054403	20.70088562191556
30	22.426626519222182	26.956134610037047	28.347906443721016	22.269332427019755
31	21.669648700498005	26.552647175307076	29.62606138401948	22.15164274017544
32	21.355903163015665	27.285609556739633	29.417365829543762	21.941121450700944
33	21.781299417730978	26.60222290258159	29.326219520758606	22.29025815892883
34	22.5898191398822	26.66710671561509	29.39770406801846	21.34537007648425
35	22.130717008266366	26.98380151732622	28.573454936646996	22.312026537760413
36	21.691557520483343	26.782268461691867	28.228812345339175	23.29736167248561
37	20.966319402507157	26.129778861359306	30.524603885725842	22.3792978504077
38	21.03541645015322	25.557902483280483	30.1588951213552	23.247785945211096
39	20.883459121793376	26.29760604009314	30.983144252726664	21.835790585386817
40	21.461795792944798	25.671519376665984	30.58401049376301	22.28267433662621
41	20.934579701759166	26.06054137255948	30.307622303178743	22.697256622502604
42	20.0906688088624	26.792380224762024	30.198218644405806	22.91873232196977
43	20.614233430050476	25.06762241553167	30.14583409405625	24.172310060361607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1986.0
1	1507.0
2	1028.0
3	1356.0
4	1684.0
5	1684.0
6	2285.0
7	2886.0
8	2610.5
9	2335.0
10	2754.5
11	3174.0
12	3174.0
13	5149.0
14	7124.0
15	8882.5
16	10641.0
17	10284.0
18	9927.0
19	9927.0
20	12230.5
21	14534.0
22	13447.0
23	12360.0
24	14031.0
25	15702.0
26	15702.0
27	16559.0
28	17416.0
29	19221.5
30	21027.0
31	23380.5
32	25734.0
33	25734.0
34	31493.0
35	37252.0
36	38395.0
37	39538.0
38	38233.5
39	36929.0
40	36929.0
41	37344.5
42	37760.0
43	40255.5
44	42751.0
45	40762.0
46	38773.0
47	38773.0
48	38418.5
49	38064.0
50	40331.5
51	42599.0
52	44689.0
53	46779.0
54	46779.0
55	47177.0
56	47575.0
57	42790.5
58	38006.0
59	36182.0
60	34358.0
61	34358.0
62	30565.5
63	26773.0
64	21550.5
65	16328.0
66	14264.0
67	12200.0
68	12200.0
69	10965.5
70	9731.0
71	9579.5
72	9428.0
73	8047.5
74	6667.0
75	6667.0
76	4079.5
77	1492.0
78	1111.0
79	730.0
80	557.5
81	385.0
82	385.0
83	287.0
84	189.0
85	139.0
86	89.0
87	67.0
88	45.0
89	45.0
90	35.5
91	26.0
92	19.5
93	13.0
94	8.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	712042.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.149056429282446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.89800569297631	52.525819972826326
2	9.35729752503239	11.443798287675811
3	2.1489022147752412	3.942100283769037
4	0.8071260654770201	1.974199892935961
5	0.4032068494425246	1.2327859194617066
6	0.2565746963052713	0.9413580349618225
7	0.18811837670672382	0.8052282862837127
8	0.1305148490826204	0.6384687897129949
9	0.09761497193826542	0.5372157084656233
>10	0.5806873287569356	6.808511509696827
>50	0.052919304895334245	2.2422827159678604
>100	0.06539792767565766	8.622060074254765
>500	0.010630051848227911	4.487382649085888
>1k	0.0027730570038855423	3.036430229360044
>5k	2.3108808365712854E-4	0.7623576455415785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5395	0.7576800244929371	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4575	0.6425182784161608	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3234	0.45418669123450583	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2587	0.3633212647568542	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	2258	0.3171161251723915	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1386	0.19465143910050248	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1151	0.16164776796874342	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1115	0.15659188643366542	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	1066	0.1497102698998093	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	1061	0.14900806413104845	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1034	0.14521615297973994	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1012	0.1421264475971923	No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA	1009	0.1417051241359358	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	966	0.13566615452459263	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	904	0.12695880299195833	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	902	0.126677920684454	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	883	0.12400953876316285	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	871	0.12232424491813686	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	837	0.1175492456905632	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	812	0.11403821684675905	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	802	0.11263380530923738	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	793	0.11136983492546787	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	785	0.11024630569545055	No Hit
CTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGA	780	0.10954409992668972	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	770	0.10813968838916807	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	770	0.10813968838916807	No Hit
TTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATCAG	768	0.10785880608166372	No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA	742	0.1042073360841074	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	737	0.10350513031534656	No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC	737	0.10350513031534656	No Hit
TATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTCTCTTC	736	0.10336468916159441	No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC	727	0.10210071877782491	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	724	0.1016793953165684	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	719	0.10097718954780759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.404411537521663E-4	0.0	0.0	0.0
2	0.0	1.404411537521663E-4	0.0	0.0	0.0
3	0.0	1.404411537521663E-4	0.0	0.0	0.0
4	0.0	1.404411537521663E-4	0.0	1.404411537521663E-4	0.0
5	0.0	1.404411537521663E-4	0.0	1.404411537521663E-4	0.0
6	1.404411537521663E-4	1.404411537521663E-4	0.0	1.404411537521663E-4	0.0
7	1.404411537521663E-4	1.404411537521663E-4	0.0	1.404411537521663E-4	0.0
8	1.404411537521663E-4	1.404411537521663E-4	0.0	1.404411537521663E-4	0.0
9	1.404411537521663E-4	1.404411537521663E-4	0.0	1.404411537521663E-4	0.0
10	2.808823075043326E-4	1.404411537521663E-4	0.0	2.808823075043326E-4	0.0
11	2.808823075043326E-4	1.404411537521663E-4	0.0	2.808823075043326E-4	0.0
12	2.808823075043326E-4	1.404411537521663E-4	0.0	4.213234612564989E-4	0.0
13	2.808823075043326E-4	1.404411537521663E-4	0.0	4.213234612564989E-4	0.0
14	2.808823075043326E-4	1.404411537521663E-4	0.0	4.213234612564989E-4	0.0
15	2.808823075043326E-4	1.404411537521663E-4	0.0	4.213234612564989E-4	0.0
16	2.808823075043326E-4	1.404411537521663E-4	0.0	4.213234612564989E-4	0.0
17	2.808823075043326E-4	1.404411537521663E-4	0.0	4.213234612564989E-4	0.0
18	2.808823075043326E-4	1.404411537521663E-4	0.0	4.213234612564989E-4	0.0
19	4.213234612564989E-4	1.404411537521663E-4	0.0	5.617646150086652E-4	0.0
20	5.617646150086652E-4	1.404411537521663E-4	0.0	0.001404411537521663	0.0
21	7.022057687608315E-4	1.404411537521663E-4	0.0	0.001404411537521663	0.0
22	8.426469225129978E-4	1.404411537521663E-4	0.0	0.0023874996137868273	0.0
23	9.830880762651641E-4	2.808823075043326E-4	0.0	0.0039323523050606565	0.0
24	9.830880762651641E-4	2.808823075043326E-4	0.0	0.006600734226351816	0.0
25	9.830880762651641E-4	2.808823075043326E-4	0.0	0.009830880762651642	0.0
26	9.830880762651641E-4	2.808823075043326E-4	0.0	0.016993379604012124	0.0
27	9.830880762651641E-4	2.808823075043326E-4	0.0	0.0629176368809705	0.0
28	9.830880762651641E-4	4.213234612564989E-4	0.0	0.15771541566368277	0.0
29	9.830880762651641E-4	4.213234612564989E-4	0.0	0.27400069097047647	0.0
30	9.830880762651641E-4	4.213234612564989E-4	0.0	0.382561702820901	0.0
31	9.830880762651641E-4	4.213234612564989E-4	0.0	0.619064605739549	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1140	0.0	31.320177	1
CGTTTAG	60	4.317917E-8	27.750002	26
GCGTTTA	70	1.92078E-7	23.785713	25
ATTAGAC	55	1.9013412E-5	23.545454	3
CGTGTAA	40	0.0019304693	23.125	1
AATGCGT	65	2.6804664E-6	22.76923	35
ATGCGTT	70	5.097423E-6	21.142857	36
TGCGTTA	70	5.097423E-6	21.142857	37
TTCGTCT	80	6.9524685E-7	20.8125	18
ACGTCCG	55	5.1404536E-4	20.181818	8
GTATCAA	1820	0.0	19.71978	2
TGCGTTT	85	1.2439468E-6	19.588234	24
TTTAGAC	125	4.110916E-10	19.24	3
GACCGTA	50	0.007032317	18.5	35
ACCTAGG	50	0.007032317	18.5	2
CGAATTA	50	0.007032317	18.5	15
CGTCCGG	60	9.23211E-4	18.5	9
ATTTTCG	90	2.1498763E-6	18.499998	15
GTGTAAG	115	6.394657E-8	17.695652	1
GATTAAT	115	6.394657E-8	17.695652	1
>>END_MODULE