FastQCFastQC Report
Fri 10 Feb 2017
ERR1630096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12993
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1751.3468790887400908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1461.1236819826060185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1130.8696990687293158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.4233048564611714No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.19241129839144153No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.16162549064881088No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1462325867774956No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11544677903486493No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1000538751635496No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1000538751635496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA352.2124308E-531.7142851
GTATCAA751.8757196E-417.2666661
AACGCAG907.522945E-414.3888886
CAACGCA907.522945E-414.3888885
TCAACGC950.001132829613.6315794
TATCAAC950.001132829613.6315792
ATCAACG950.001132829613.6315793
ACGCAGA1200.00653880410.7916677
CGCAGAG1200.00653880410.7916678
Produced by FastQC (version 0.11.5)