##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12993
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74309243438775	34.0	31.0	34.0	31.0	34.0
2	32.93527283922112	34.0	31.0	34.0	31.0	34.0
3	33.08027399368891	34.0	33.0	34.0	31.0	34.0
4	36.3475717694143	37.0	37.0	37.0	35.0	37.0
5	36.373123989840686	37.0	37.0	37.0	35.0	37.0
6	36.419379665973985	37.0	37.0	37.0	35.0	37.0
7	36.356037866543524	37.0	37.0	37.0	35.0	37.0
8	36.3651196798276	37.0	37.0	37.0	35.0	37.0
9	38.11267605633803	39.0	39.0	39.0	37.0	39.0
10	38.109982298160546	39.0	39.0	39.0	37.0	39.0
11	38.13676595089664	39.0	39.0	39.0	37.0	39.0
12	38.08466097129224	39.0	39.0	39.0	37.0	39.0
13	38.107365504502425	39.0	38.0	39.0	37.0	39.0
14	39.493650427153085	41.0	39.0	41.0	37.0	41.0
15	39.47833448780112	41.0	39.0	41.0	37.0	41.0
16	39.37497113830524	41.0	39.0	41.0	36.0	41.0
17	39.36481182175017	41.0	39.0	41.0	36.0	41.0
18	39.27237743400293	41.0	39.0	41.0	36.0	41.0
19	39.301546986839064	41.0	39.0	41.0	36.0	41.0
20	39.515123528053564	41.0	39.0	41.0	37.0	41.0
21	39.499884553220966	41.0	39.0	41.0	37.0	41.0
22	39.48556915262064	41.0	39.0	41.0	36.0	41.0
23	39.399599784499344	41.0	39.0	41.0	36.0	41.0
24	39.324790271684755	41.0	39.0	41.0	36.0	41.0
25	39.293773570384054	41.0	39.0	41.0	36.0	41.0
26	39.044023705071965	41.0	39.0	41.0	35.0	41.0
27	38.92873085507581	41.0	39.0	41.0	35.0	41.0
28	38.8817824982683	41.0	39.0	41.0	35.0	41.0
29	38.72508273685831	40.0	38.0	41.0	35.0	41.0
30	38.49026398830139	40.0	38.0	41.0	35.0	41.0
31	38.209343492649886	40.0	38.0	41.0	34.0	41.0
32	38.10952051104441	40.0	37.0	41.0	34.0	41.0
33	37.94897252366659	40.0	37.0	41.0	34.0	41.0
34	37.79873778188255	40.0	37.0	41.0	34.0	41.0
35	37.59963057030709	40.0	36.0	41.0	33.0	41.0
36	37.511352266605094	40.0	36.0	41.0	33.0	41.0
37	37.368121296082506	40.0	36.0	41.0	33.0	41.0
38	37.1708612329716	40.0	36.0	41.0	32.0	41.0
39	37.067651812514434	40.0	35.0	41.0	31.0	41.0
40	36.83367967367044	40.0	35.0	41.0	31.0	41.0
41	36.64696374971138	40.0	35.0	41.0	31.0	41.0
42	36.450242438235975	40.0	35.0	41.0	30.0	41.0
43	36.06187947356269	40.0	35.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	5.0
22	4.0
23	13.0
24	17.0
25	26.0
26	62.0
27	73.0
28	97.0
29	117.0
30	137.0
31	187.0
32	220.0
33	313.0
34	370.0
35	494.0
36	818.0
37	1538.0
38	2333.0
39	6167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53936735165089	20.157007619487416	13.768952512891557	21.534672515970136
2	18.640806588162857	23.612714538597707	35.48833987531748	22.258138997921957
3	19.918417609482027	25.87547140768106	32.278919418148234	21.927191564688677
4	14.007542522896946	17.178480720387903	36.72746863695836	32.08650811975679
5	13.53036250288617	37.98968675440622	33.856692064958054	14.623258677749556
6	31.647810359424305	36.373431847918106	15.662279689063341	16.316478103594243
7	26.152543677364736	31.801739398137457	22.635265142769185	19.410451781728625
8	25.860078503809742	34.241514661740936	20.834295389825293	19.06411144462403
9	24.559378126683598	14.992688370661126	22.81228353728931	37.635649965365964
10	17.70953590394828	27.35319017932733	33.140922034941894	21.796351881782495
11	33.17170784268452	23.474178403755868	22.435157392442086	20.918956361117523
12	20.1416147156161	28.592318940968216	30.07773416455014	21.188332178865544
13	30.193180943585006	21.63472639113369	26.57584853382591	21.5962441314554
14	20.241668590779653	23.99753713538059	28.092049565150468	27.668744708689296
15	24.84414684830293	29.169552836142536	24.266912953128607	21.71938736242592
16	21.434618640806587	27.922727622565997	27.360886631262986	23.281767105364427
17	20.649580543369506	29.177249288078194	28.13822827676441	22.034941891787884
18	21.1421534672516	26.799045639959978	29.284999615177405	22.77380127761102
19	22.81228353728931	26.39883013930578	30.44716385746171	20.3417224659432
20	22.21965673824367	26.806742091895636	30.624182251981836	20.349418917878857
21	21.511583160163163	26.945278226737475	30.331717078426845	21.211421534672517
22	20.1416147156161	27.61486954513969	31.478488416839838	20.76502732240437
23	20.988224428538444	26.62202724543985	31.062880012314327	21.32686831370738
24	21.203725082736856	26.945278226737475	30.20857384745632	21.642422843069344
25	20.63418763949819	27.168475332871544	30.831986454244593	21.36535057338567
26	21.03440314015239	27.791887939659816	30.631878703917494	20.5418302162703
27	20.349418917878857	26.945278226737475	31.001308396829064	21.703994458554607
28	20.603401831755562	27.55329792965443	30.21627029939198	21.62702993919803
29	20.056953744323867	28.51535442161164	30.39328869391211	21.03440314015239
30	20.249365042715308	27.961209882244287	31.00900484876472	20.780420226275687
31	20.841991841760947	28.04587085353652	30.7396290310167	20.37250827368583
32	20.08004310013084	28.42299699838374	30.993611944893406	20.50334795659201
33	20.21088278303702	27.607173093204036	30.354806434233815	21.827137689525127
34	20.233972138843992	27.861156007080734	30.2316632032633	21.673208650811976
35	19.57977372431309	27.653351804817977	30.470253213268684	22.296621257600247
36	20.1416147156161	28.099746017086126	30.293234818748555	21.46540444854922
37	20.072346648195182	27.607173093204036	31.1937196952205	21.12676056338028
38	20.126221811744784	26.983760486415765	31.355345185869314	21.534672515970136
39	19.625952435927037	26.8683137073809	31.978757792657586	21.526976064034482
40	19.325790810436388	26.968367582544445	31.470791964904176	22.235049642114983
41	19.179558223658894	27.08381436157931	31.40152389748326	22.335103517278533
42	18.07896559685985	27.522512121911795	32.15577618717771	22.24274609405064
43	18.99484337720311	26.483491110598013	32.0480258600785	22.473639652120372
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	20.5
2	14.0
3	29.5
4	45.0
5	45.0
6	48.5
7	52.0
8	48.0
9	44.0
10	65.5
11	87.0
12	87.0
13	144.0
14	201.0
15	277.0
16	353.0
17	334.5
18	316.0
19	316.0
20	358.5
21	401.0
22	305.0
23	209.0
24	239.5
25	270.0
26	270.0
27	290.0
28	310.0
29	354.5
30	399.0
31	453.5
32	508.0
33	508.0
34	550.5
35	593.0
36	632.0
37	671.0
38	686.0
39	701.0
40	701.0
41	705.5
42	710.0
43	693.0
44	676.0
45	706.0
46	736.0
47	736.0
48	753.5
49	771.0
50	781.5
51	792.0
52	763.0
53	734.0
54	734.0
55	713.5
56	693.0
57	654.0
58	615.0
59	597.5
60	580.0
61	580.0
62	509.0
63	438.0
64	386.5
65	335.0
66	292.0
67	249.0
68	249.0
69	221.5
70	194.0
71	158.0
72	122.0
73	102.5
74	83.0
75	83.0
76	54.5
77	26.0
78	22.0
79	18.0
80	12.5
81	7.0
82	7.0
83	7.5
84	8.0
85	5.5
86	3.0
87	2.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12993.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.31786346494266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.58585428206221	86.331101362272
2	3.5875585854282064	6.4804125298237505
3	0.4942479761397529	1.3391826368044333
4	0.06817213463996591	0.24628646194104517
5	0.08521516829995739	0.38482259678288305
6	0.025564550489987216	0.1385361348418379
7	0.025564550489987216	0.16162549064881088
8	0.034086067319982954	0.24628646194104517
9	0.0	0.0
>10	0.05965061780997018	0.9081813284076041
>50	0.008521516829995739	0.4233048564611714
>100	0.025564550489987216	3.3402601400754253
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	175	1.3468790887400908	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	1.1236819826060185	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	113	0.8696990687293158	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.4233048564611714	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25	0.19241129839144153	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21	0.16162549064881088	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.1462325867774956	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.11544677903486493	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.1000538751635496	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.1000538751635496	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.007696451935657662	0.0	0.0	0.0	0.0
19	0.007696451935657662	0.0	0.0	0.0	0.0
20	0.007696451935657662	0.0	0.0	0.0	0.0
21	0.007696451935657662	0.0	0.0	0.0	0.0
22	0.007696451935657662	0.0	0.0	0.0	0.0
23	0.007696451935657662	0.0	0.0	0.007696451935657662	0.0
24	0.007696451935657662	0.0	0.0	0.023089355806972984	0.0
25	0.007696451935657662	0.0	0.0	0.04617871161394597	0.0
26	0.007696451935657662	0.0	0.0	0.06157161548526129	0.0
27	0.007696451935657662	0.0	0.0	0.14623258677749557	0.0
28	0.007696451935657662	0.0	0.0	0.31555452936196415	0.0
29	0.007696451935657662	0.0	0.0	0.4848764719464327	0.0
30	0.007696451935657662	0.0	0.0	0.7465558377587932	0.0
31	0.007696451935657662	0.0	0.0	1.0851997229277304	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35	2.2124308E-5	31.714285	1
GTATCAA	75	1.8757196E-4	17.266666	1
AACGCAG	90	7.522945E-4	14.388888	6
CAACGCA	90	7.522945E-4	14.388888	5
TCAACGC	95	0.0011328296	13.631579	4
TATCAAC	95	0.0011328296	13.631579	2
ATCAACG	95	0.0011328296	13.631579	3
ACGCAGA	120	0.006538804	10.791667	7
CGCAGAG	120	0.006538804	10.791667	8
>>END_MODULE