FastQCFastQC Report
Fri 10 Feb 2017
ERR1630095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24326
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3551.4593439118638494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3361.3812381813697279No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2430.9989311847406068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.5837375647455397No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.2384280194031078No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT570.23431719148236455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.1767656005919592No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT410.16854394475047274RNA PCR Primer, Index 23 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1644331168297295No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.1521006330674998No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.14798980514675655No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA330.13565732138452682No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.12743566554304037No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11510318178081065No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.11099235386006741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1400.027.751
GTATCAA2050.018.9512182
TACGGGG500.00684339718.4999984
ATCAACG2700.014.3888884
AACGCAG2750.014.1272727
TATCAAC2750.014.1272723
TCAACGC2850.013.6315785
CAACGCA2900.013.3965516
CGCAGAG2950.013.1694929
ACGCAGA3100.012.5322578
CAGAGTA3100.012.53225711
AGAGTAC3150.012.33333312
GCAGAGT3200.012.14062510
AGTACGG1706.353612E-510.8823532
GAGTACG1706.353612E-510.8823531
GTACGGG1706.353612E-510.8823533
AGTACTT2104.92393E-59.69047514
GAGTACT2104.92393E-59.69047513
CTTTTTT2605.2577954E-58.53846218
GTACTTT2452.642739E-48.30612215
Produced by FastQC (version 0.11.5)